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Studying single plant light-harvesting complexes in near-native environments
Thesis (PhD (Physics))--University of Pretoria, 2023.
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University of Pretoria
2024
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| _version_ | 1867613609233743872 |
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| access_status_str | Open Access |
| author2 | Kruger, T.P.J. (Tjaart |
| author_browse | Kruger, T.P.J. (Tjaart |
| author_facet | Kruger, T.P.J. (Tjaart |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Physics))--University of Pretoria, 2023. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/94990 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:52.072Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/94990 Studying single plant light-harvesting complexes in near-native environments Kruger, T.P.J. (Tjaart bertus.mooikloof@gmail.com Kirchhoff, Helmut van Heerden, Bertus UCTD Single-molecule spectroscopy Light-harvesting complexes Single-particle tracking Photosynthesis Proteoliposomes Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-12: Responsible consumption and production Natural and agricultural sciences theses SDG-12 SDG-13: Climate action Natural and agricultural sciences theses SDG-13 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Thesis (PhD (Physics))--University of Pretoria, 2023. Photosynthesis in plants begins with light absorption by light-harvesting complexes, the main one being light-harvesting complex II (LHCII). This protein is a key component of a critical photoprotective process called non-photochemical quenching (NPQ). Single-molecule spectroscopy (SMS) has revealed that isolated LHCII complexes have complex, individual behaviour, and this behaviour can be directy related to NPQ. The physiological applicability of these findings is, however, limited by the artificial environment typically used for SMS. Applying SMS in vivo is immensely challenging, however, in part because the complexes are crowded and in constant motion. New approaches are therefore needed which enable the study of LHCII (and similar proteins) in controllable environments that mimic the native one. This thesis develops two such approaches, namely real-time feedback-driven single-particle tracking (RT-FD-SPT) and proteoliposomes, and applies them to LHCII. Different RT-FD-SPT methods were investigated using theoretical modeling, illuminating fundamental aspects of performance and aiding in the selection of an appropriate method. New data analysis code was developed for fluorescence lifetime analysis and applied to measurements on LHCII. The first measurements of photon antibunching from LHCII are reported. An RT-FD-SPT setup with unique spectroscopic measurement capabilities was constructed and used, for the first time, on light-harvesting complexes. LHCII aggregation was investigated using fluorescence correlation spectroscopy (FCS) and RT-FD-SPT. Lastly, a proteoliposome protocol was optimised which allows protein-lipid interactions to be studied at the single-molecule level. NRF NITheCS CSIR Fulbright Physics PhD (Physics) Restricted Faculty of Natural and Agricultural Sciences 2024-02-29T08:31:42Z 2024-02-29T08:31:42Z 2024-04-17 2023-12-14 Thesis * A2024 http://hdl.handle.net/2263/94990 10.25403/UPresearchdata.25304074 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Single-molecule spectroscopy Light-harvesting complexes Single-particle tracking Photosynthesis Proteoliposomes Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-12: Responsible consumption and production Natural and agricultural sciences theses SDG-12 SDG-13: Climate action Natural and agricultural sciences theses SDG-13 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Studying single plant light-harvesting complexes in near-native environments |
| title | Studying single plant light-harvesting complexes in near-native environments |
| title_full | Studying single plant light-harvesting complexes in near-native environments |
| title_fullStr | Studying single plant light-harvesting complexes in near-native environments |
| title_full_unstemmed | Studying single plant light-harvesting complexes in near-native environments |
| title_short | Studying single plant light-harvesting complexes in near-native environments |
| title_sort | studying single plant light harvesting complexes in near native environments |
| topic | UCTD Single-molecule spectroscopy Light-harvesting complexes Single-particle tracking Photosynthesis Proteoliposomes Sustainable Development Goals (SDGs) SDG-02: Zero hunger Natural and agricultural sciences theses SDG-02 SDG-12: Responsible consumption and production Natural and agricultural sciences theses SDG-12 SDG-13: Climate action Natural and agricultural sciences theses SDG-13 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/94990 |