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Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio
Dissertation (MSc (Entomology))--University of Pretoria, 2024.
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| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613626350698496 |
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| access_status_str | Open Access |
| author2 | Dittrich-Schröder, Gudrun |
| author_browse | Dittrich-Schröder, Gudrun |
| author_facet | Dittrich-Schröder, Gudrun |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Entomology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/95031 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:39:08.341Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/95031 Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio Dittrich-Schröder, Gudrun elmarie.vandermerwe@up.ac.za Slippers, Bernard Van der Merwe, Elmarie UCTD CRISPR/Cas9 Sirex noctilio Pest management Rearing Gene editing Artificial diet Microinjection Hymenoptera Pine forestry Sustainable Development Goals (SDGs) SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Dissertation (MSc (Entomology))--University of Pretoria, 2024. Sirex noctilio (Hymenoptera: Siricidae) is an invasive pest in South African pine plantations. Current pest management strategies for S. noctilio, such as silvicultural practices or biological control, are not always efficient and gene editing could add to the toolbox of management options. Gene editing has emerged as a new and precision approach for genetic pest control. This approach is facilitated by the discovery of Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), with its associated Cas9 nuclease, as a more accessible approach to do gene editing in a wide range of insect pests. Currently, the only feasible option to deliver CRISPR/Cas9 into insects is through microinjection of embryos. However, access to S. noctilio embryos is challenging as they are oviposited into the tree, and if obtained via dissection, will not develop further. In this study, an egg activation protocol was developed for S. noctilio to enable microinjection of large numbers of embryos. In addition, various artificial diets were envaulted with the aim to rear first instar larvae emerging from activated eggs and microinjected embryos. Sirex noctilio eggs from two climatically different regions in South Africa were successfully activated for the first time. Microinjected embryos developed abnormally and had a very low survival rate, and DNA sequencing results revealed that no microinjected embryo was genetically modified. The optimal artificial diet to rear first instar S. noctilio larvae was a modified Anoplophora glabripennis (Coleoptera: Cerambycidae) diet. The results in this study, from rearing to gene editing, are extendable to other insect species with similar characteristics and may enable progress towards their gene editing. Most importantly, this study has unlocked new research opportunities in S. noctilio that may help to develop additional tools for pest management. FLR\R1\201229 TPCP/CPHB Zoology and Entomology MSc (Entomology) Restricted Faculty of Natural and Agricultural Sciences 2024-03-01T11:32:07Z 2024-03-01T11:32:07Z 2024-04 2024-01-15 Dissertation * A2024 http://hdl.handle.net/2263/95031 https://doi.org/10.25403/UPresearchdata.25310785 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD CRISPR/Cas9 Sirex noctilio Pest management Rearing Gene editing Artificial diet Microinjection Hymenoptera Pine forestry Sustainable Development Goals (SDGs) SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title | Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title_full | Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title_fullStr | Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title_full_unstemmed | Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title_short | Development of CRISPR/Cas9 gene editing tools for the insect pest sirex noctilio |
| title_sort | development of crispr cas9 gene editing tools for the insect pest sirex noctilio |
| topic | UCTD CRISPR/Cas9 Sirex noctilio Pest management Rearing Gene editing Artificial diet Microinjection Hymenoptera Pine forestry Sustainable Development Goals (SDGs) SDG-09: Industry, innovation and infrastructure Natural and agricultural sciences theses SDG-09 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/95031 https://doi.org/10.25403/UPresearchdata.25310785 |