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Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum
Dissertation (MSc (Microbiology))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613690529841152 |
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| access_status_str | Open Access |
| author2 | Hammerbacher, Almuth |
| author_browse | Hammerbacher, Almuth |
| author_facet | Hammerbacher, Almuth |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/95097 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:09.614Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/95097 Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum Hammerbacher, Almuth u18107339@tuks.co.za Bose, Tanay Wingfield, Brenda D. Nkomo, Tiphany UCTD Orchid mycorrhiza Fungal biodiversity High-throughput sequencing Fungal genome Epidendrum hybrids Mycorrhizae Sustainable Development Goals (SDGs) SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Dissertation (MSc (Microbiology))--University of Pretoria, 2024. Orchids are known for their exceptional floral morphologies, as well as their symbiotic relationships with specialized fungi known as orchid mycorrhizae. Existing literature indicates a significant gap in our understanding of the complex relationship between orchids and their mycorrhizal partners. Many common orchid mycorrhizae are recognized as pathogens of crops or are well-known saprophytes. It is not clearly understood how orchids form symbiotic relationships with these non-mycorrhizal fungi. Similarly, the diversity of fungi that form symbiotic associations with orchids is also sparse. Thus, this thesis contains four chapters that are aimed to partially reduce the aforementioned knowledge gaps. ‘Chapter One’ of this thesis focused on reviewing the existing knowledge of the biochemical and molecular interactions between plants and mycorrhizal fungi, with special reference to the significant knowledge gaps about the orchid-mycorrhizae relationship and suggestions on how these gaps can be filled. 'Chapter Two’ of the thesis aimed to isolate orchid mycorrhizal fungi associated with various Epidendrum hybrids popularly available as horticultural plants in South Africa using a culture-dependent process. In this process, various fungi were isolated from the roots of sampled Epidendrum plants as well as a previously undescribed orchid mycorrhiza. ‘Chapter Three’ aimed to understand if orchids from the same genus associate with overlapping fungi across their native and introduced regions. To answer this question, root samples of Epidendrum hybrids were procured from Brazil, Columbia, Germany, Spain and six provinces of South Africa. Then, high-throughput sequencing platform was used in conjunction with fungal-specific primers to catalogue the biodiversity of fungi associated with DNA extracted from these root samples. Following that, bioinformatics and statistical pipeline were employed to explore the data to answer the research objective of this chapter. ‘Chapter Four’ aimed to improve the progress of future genomic studies associated with Rhizoctonia-like fungi. To address this, the whole genome of an orchid mycorrhizal fungus, Ceratobasidium papillatum CBS 570.83 was sequenced, assembled, and annotated. NRF Sponsored Microbiology and Plant Pathology MSc (Microbiology) Restricted Faculty of Natural and Agricultural Sciences 2024-03-07T12:42:24Z 2024-03-07T12:42:24Z 2024-03-06 2024-03-06 Dissertation * A2024 http://hdl.handle.net/2263/95097 DOI: https://doi.org/10.25403/UPresearchdata.25347727.v1 10.25403/UPresearchdata.25347727 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Orchid mycorrhiza Fungal biodiversity High-throughput sequencing Fungal genome Epidendrum hybrids Mycorrhizae Sustainable Development Goals (SDGs) SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title | Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title_full | Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title_fullStr | Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title_full_unstemmed | Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title_short | Exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus Epidendrum |
| title_sort | exploring the mycorrhizal interactions and deciphering the fungal diversity associated with orchid hybrids from the genus epidendrum |
| topic | UCTD Orchid mycorrhiza Fungal biodiversity High-throughput sequencing Fungal genome Epidendrum hybrids Mycorrhizae Sustainable Development Goals (SDGs) SDG-15: Life on land Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/95097 |