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Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia
Thesis (PhD)--University of Pretoria, 2021.
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| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613581648855040 |
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| access_status_str | Open Access |
| author2 | Van Heerden, Henriette |
| author_browse | Van Heerden, Henriette |
| author_facet | Van Heerden, Henriette |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 2021. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/95625 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:25.653Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/95625 Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia Van Heerden, Henriette omuzembe@gmail.com Fasina, Folorunso Oludayo Madzingira, Oscar UCTD Epidemiology Brucellosis Namibia Brucella abortus Amos-PCR Thesis (PhD)--University of Pretoria, 2021. This study assessed knowledge, attitudes, and practices regarding brucellosis among cattle farmers (n=264), meat handlers (n=143) and medical professionals (n=124); estimated seroprevalence of Brucella infection in cattle (n=49718) (2004-2018) and humans (n=971) (2012-2017) retrospectively, and prospectively (n=304) at a major abattoir in Namibia. Molecular characterisation of Brucella species was performed on DNA extracted from spleen and lymph nodes from seropositive cattle. Overall awareness of brucellosis was 43.50% (231/531), with highest awareness among medical professionals (73.40%, 91/124) and the lowest in meat handlers (14.00%, 20/143). Medical professionals (98.40%, 122/124) did not consider brucellosis in the differential diagnosis of persistent fever in humans. Seroprevalence of human brucellosis was 11.64% (113/971, 95% CI: 9.77-13.81), with positive cases clustered in the 30-40-year age group and in females (64.00%) (z=-5.24, p<0.01). Individual cattle and herd prevalence of brucellosis was 0.49% (244/49718, 95% CI: 0.43%-0.56%) and 9.26% (78/842, 95% CI: 7.49%-11.41%) respectively, with more seropositive communal herds (33.09%) and cattle (10.27%) than commercial herds (4.67%) and cattle (0.24%) (p<0.05). Seroprevalence of brucellosis in the abattoir was 2.30% (7/304; 95% CI: 1.10-4.70%) based on RBT, and 1.64% (5/304; 95% CI: 0.70-3.8%) after confirmation with CFT, while herd prevalence was 9.62% (5/52). Brucella DNA was detected in lymph nodes (6/7, 85.71%) and spleens (6/7, 85.71%) from seropositive cattle using ITS-PCR. From cultures, Brucella abortus isolates were confirmed from lymph nodes (4/7, 57.14%) and spleen (6/7, 85.71%) by AMOS-PCR. Targeted public health education, better enforcement of current control measures and the use of protective gear are recommended to prevent human and animal infection. Veterinary Tropical Diseases PhD Unrestricted 2024-04-17T14:09:48Z 2024-04-17T14:09:48Z 2021 2021-03 Thesis * S2021 http://hdl.handle.net/2263/95625 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Epidemiology Brucellosis Namibia Brucella abortus Amos-PCR Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title | Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title_full | Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title_fullStr | Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title_full_unstemmed | Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title_short | Epidemiology and molecular confirmation of Brucella spp. in cattle in Namibia |
| title_sort | epidemiology and molecular confirmation of brucella spp in cattle in namibia |
| topic | UCTD Epidemiology Brucellosis Namibia Brucella abortus Amos-PCR |
| url | http://hdl.handle.net/2263/95625 |