Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2
Dissertation (MSc (Entomology))--University of Pretoria, 2024.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2024
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613691865726976 |
|---|---|
| access_status_str | Open Access |
| author2 | Dittrich-Schröder, Gudrun |
| author_browse | Dittrich-Schröder, Gudrun |
| author_facet | Dittrich-Schröder, Gudrun |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Entomology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/96946 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:10.900Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/96946 Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 Dittrich-Schröder, Gudrun Jade.ashmore13@gmail.com Slippers, Bernard Doung, Tuan Ashmore, Jade Sabrina UCTD Sustainable Development Goals (SDGs) Insect sex determination Tyrosine 3-monooxygenase Embryo microinjection Genome assembly and annotation Cytochrome P450 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Dissertation (MSc (Entomology))--University of Pretoria, 2024. Literature review of insect sex determination pathway and CRISPR/Cas9 gene editing tool in application for genetic-based pest control methods. The de novo genome of Gonipterus sp. n. 2 was sequenced with long and short read sequencing, assembled and annotated using RNA sequencing data and Protein sequences from other Coleopteran species with published, assembled genomes. The expansion and contraction of gene families, with a focus on the cytochrome P450 detoxification gene, was assessed. A CRISPR/Cas9 transformation protocol for Gonipterus sp. n. 2 embryos with microinjection. The Tyrosine 3-monooxygenase (Pale) gene was targeted to assess the efficiency of the embryo transformations. This work was supported by Future Leaders—African Independent Research (FLAIR) grant (Grant number: FLR\R1\201229). The FLAIR grant is a partnership between the African Academy of Sciences (AAS) and The Royal Society, supported by the Global Challenges Research Fund (GCRF). Zoology and Entomology MSc (Entomology) Unrestricted Faculty of Natural and Agricultural Sciences SDG-15: Life on land 2024-07-12T08:42:30Z 2024-07-12T08:42:30Z 2024-10-01 2024-03-21 Dissertation * S2024 http://hdl.handle.net/2263/96946 DOI: https://doi.org/10.25403/UPresearchdata.26246402.v1 10.25403/UPresearchdata.26246402 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Insect sex determination Tyrosine 3-monooxygenase Embryo microinjection Genome assembly and annotation Cytochrome P450 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title | Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title_full | Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title_fullStr | Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title_full_unstemmed | Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title_short | Development of CRISPR/Cas9 gene editing tools for the insect pest Gonipterus sp. n. 2 |
| title_sort | development of crispr cas9 gene editing tools for the insect pest gonipterus sp n 2 |
| topic | UCTD Sustainable Development Goals (SDGs) Insect sex determination Tyrosine 3-monooxygenase Embryo microinjection Genome assembly and annotation Cytochrome P450 SDG-15: Life on land Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/96946 |