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Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa
Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2019.
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| Format: | Thesis |
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University of Pretoria
2024
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| _version_ | 1867613465122701312 |
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| access_status_str | Open Access |
| author2 | Ehlers, M.M. (Marthie Magdaleen) |
| author_browse | Ehlers, M.M. (Marthie Magdaleen) |
| author_facet | Ehlers, M.M. (Marthie Magdaleen) |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/97506 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:34.553Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/97506 Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa Ehlers, M.M. (Marthie Magdaleen) keitumetseg8@gmail.com Kock, Marleen Magdalena Strydom, Kathy-Anne Gama, Keitumetse B. UCTD Burn wound patients Antibiotic resistance Virulence Neonates Methicillin-resistant Staphylococcus aureus (MRSA) Health sciences theses SDG-03 Dissertation (MSc (Medical Microbiology))--University of Pretoria, 2019. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the leading causes of nosocomial infections worldwide. It is an ESKAPE pathogen and is known for causing difficult-to-treat infections due to its antibiotic resistance. In addition to its antibiotic resistance, this bacterium has a large arsenal of virulence factors that allow this pathogen to cause disease and to evade the host immune system. An increase in the number of reports of MRSA isolates from the burn unit and neonatal wards from various hospitals across the Gauteng province prompted this study. The aim of this study was to molecularly characterise the MRSA isolates associated with outbreaks in the burn and neonatal wards at four hospitals in Gauteng, South Africa using multiplex polymerase chain reaction (M-PCR) assays, pulsedfield gel electrophoresis (PFGE) and whole genome sequencing (WGS). The study also aimed to determine the antibiotic and virulence gene profiles associated with these MRSA strains. viii To identify MRSA, a M-PCR assay was performed to confirm the presence of the Staphylococcus 16S rRNA gene, the S. aureus-specific nuclease (nuc) gene and the methicillin A (mecA) gene that confers resistance to beta-lactam antibiotics. The isolates were also screened for the Panton-Valentine Leukocidin (pvl) gene, which encodes a pore forming toxin associated with severe disease. All 85 isolates were confirmed to be MRSA and none of the isolates were pvl-positive. Susceptibility testing of the MRSA isolates revealed that the isolates were resistant to antibiotics such as penicillin (100%), cloxacillin (100%), gentamicin (98%), clindamycin (97%), erythromycin (97%), ciprofloxacin (91%) and tetracycline (84%). Susceptibility to vancomycin, teicoplanin, linezolid and fusidic acid was observed. The dendrogram constructed based on the PFGE banding profiles revealed that the MRSA isolates clustered into three major pulsotypes. The largest pulsotype, Pulsotype A, consisted of 32 MRSA isolates that were recovered from burn and neonatal wards. Pulsotypes B and C were made up of five isolates each and only consisted of isolates from the neonatal wards. All three pulsotypes were composed of MRSA isolates from different hospitals, recovered between 2015 and 2019. Five representative isolates were selected based on their clustering and antibiotic resistance and sent for WGS. Three clones, ST239-MRSA-III, ST5-MRSA-I and ST612-MRSA-IV were identified using WGS data. These clones were associated with spa types t037, t045 and t1257 respectively. The clone ST239-MRSA-III-t037 was detected in three different hospitals. The virulence factors detected in the five isolates included staphylococcal enterotoxins A (SEA), SEB, SEG, SEK, SEN, SEO, and SEQ and the bicomponent pore-forming leukocidins, gamma-hemolysin and leucocidin ED. The immune evasion complex (IEC) genes identified were the staphylococcal complement inhibitor, staphylokinase and SEA. The movement of patients and healthcare workers between wards and hospitals may have resulted in intra- and inter-hospital spread of MRSA. The study emphasises the importance of having infection control programs in place and adhering to them. The importance of continuous surveillance is also emphasised. em2026 Medical Microbiology MSc (Medical Microbiology) Unrestricted Faculty of Health Sciences SDG-03: Good health and well-being 2024-08-07T13:52:50Z 2024-08-07T13:52:50Z 2020-05 2019-12 Dissertation * A2020 http://hdl.handle.net/2263/97506 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Burn wound patients Antibiotic resistance Virulence Neonates Methicillin-resistant Staphylococcus aureus (MRSA) Health sciences theses SDG-03 Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title | Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title_full | Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title_fullStr | Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title_full_unstemmed | Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title_short | Molecular characterisation of methicillin-resistant Staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in Gauteng, South Africa |
| title_sort | molecular characterisation of methicillin resistant staphylococcus aureus isolates associated with outbreaks in burn wound and neonatal ward patients at healthcare centres in gauteng south africa |
| topic | UCTD Burn wound patients Antibiotic resistance Virulence Neonates Methicillin-resistant Staphylococcus aureus (MRSA) Health sciences theses SDG-03 |
| url | http://hdl.handle.net/2263/97506 |