Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum
Dissertation (MSc (Biotechnology))--University of Pretoria, 2015.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2024
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613616388177920 |
|---|---|
| access_status_str | Open Access |
| author2 | Berger, David Kenneth |
| author_browse | Berger, David Kenneth |
| author_facet | Berger, David Kenneth |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Biotechnology))--University of Pretoria, 2015. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/97795 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:58.622Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/97795 Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum Berger, David Kenneth dave.berger@fabi.up.ac.za Naidoo, Sanushka Kgoale, Degracious Moloko UCTD Arabidopsis thaliana-Ralstonia solanacearum pathosystem Gene expression profiling Arabidopsis defence genes Bacterial wilt pathogen Ralstonia solanacearum Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 Dissertation (MSc (Biotechnology))--University of Pretoria, 2015. Arabidopsis thaliana is a model plant widely used to gain insights in the so called plant-pathogen interactions. This is so because plants are sessile organisms living in an environment rich in disease causing microbes. In contrast, plants cannot relocate to avoid pathogen attacks like animals. As a result, plants rely on their immune responses to rapidly detect invading pathogens so as to fight and defend themselves against pathogens. Over the years, Ralstonia solanacearum has been recognised as an economically important causal agent of bacterial wilt in a vast distribution of plants ranging from important solanaceous crops, leguminous plants, a few monocotyledonous plants (Genin & Boucher, 2002) to major forest trees such as Eucalyptus (Coutinho et al., 2000). It has been documented that R. solanacearum affects over 200 plant species representing 50 botanical families (Hayward, 1991). Thus it is of utmost importance to gain as much insight into this devastating phytopathogen in order to accomplish better control methods. Chemical control is not an option for bacterial diseases, in contrast to fungal diseases. Integrated Pest Management (IPM) of bacterial wilt is considered to be a sustainable approach (Smith, 2000). Hence there is a need to generate disease resistant plants as part of IPM of bacterial wilt across the world. The first step in understanding resistance is to study compatible host plant responses to infection with R. solanacearum. A fascinating discovery was that A. thaliana is a compatible host to bacterial wilt. This has built a strong foundation, a step forward to gaining knowledge as to what happens in a host plant upon R. solanacearum infection. The study of the A. thaliana - R. solanacearum pathosystem has revealed that different ecotypes of Arabidopsis have different responses to the bacterium. Resistant and tolerant ecotypes of Arabidopsis are due to the possession of the recessive gene, AtRRS1-R on chromosome five. The RRS1-R protein physically interacts with an effector protein from the Type III Secretion System (T3SS) of R. solanacearum called PopP2 to elicit Effector Triggered Immunity (ETI) (Coutinho et al., 2000; Deslandes et al., 1998; Deslandes et al., 2002; Deslandes et al., 2003). The interaction thereof is strongly suggested to localise in the nucleus of the host plant involving at least two more A. thaliana proteins, RPS4 (Narusaka et al., 2009) and RD9 (Rivas, 2012). This further suggests that Arabidopsis responses to bacterial wilt involve an extensive cascade of distinct genes. National Research Foundation (NRF) bs2026 Plant Science MSc (Biotechnology) Unrestricted SDG-02: Zero hunger SDG-09: Industry, innovation and infrastructure SDG-15: Life on land 2024-08-21T13:57:39Z 2024-08-21T13:57:39Z 2015 2015 Dissertation * http://hdl.handle.net/2263/97795 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Arabidopsis thaliana-Ralstonia solanacearum pathosystem Gene expression profiling Arabidopsis defence genes Bacterial wilt pathogen Ralstonia solanacearum Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title | Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title_full | Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title_fullStr | Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title_full_unstemmed | Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title_short | Gene expression study of candidate Arabidopsis defence genes in response to the bacterial wilt pathogen, Ralstonia solanacearum |
| title_sort | gene expression study of candidate arabidopsis defence genes in response to the bacterial wilt pathogen ralstonia solanacearum |
| topic | UCTD Arabidopsis thaliana-Ralstonia solanacearum pathosystem Gene expression profiling Arabidopsis defence genes Bacterial wilt pathogen Ralstonia solanacearum Natural and agricultural sciences theses SDG-02 Natural and agricultural sciences theses SDG-09 Natural and agricultural sciences theses SDG-15 |
| url | http://hdl.handle.net/2263/97795 |