Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Characterisation of secondary metabolite pathways in the Ceratocystidaceae
Thesis (PhD (Microbiology))--University of Pretoria, 2019.
Saved in:
| Other Authors: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
University of Pretoria
2024
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613576656584704 |
|---|---|
| access_status_str | Open Access |
| author2 | Wingfield, Brenda D. |
| author_browse | Wingfield, Brenda D. |
| author_facet | Wingfield, Brenda D. |
| collection | Thesis |
| dc_rights_str_mv | © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD (Microbiology))--University of Pretoria, 2019. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/98338 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:21.029Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/98338 Characterisation of secondary metabolite pathways in the Ceratocystidaceae Wingfield, Brenda D. Steenkamp, Emma Theodora Van der Nest, Magrieta Aletta Sayari, Mohammad UCTD Ceratocystidaceae Secondary metabolite pathways Thesis (PhD (Microbiology))--University of Pretoria, 2019. Members of Ceratocystistidaceae (phylum Ascomycota, class Sordariomycetes) include fungal pathogens that cause diseases on a broad spectrum of hosts, leading to substantial economic losses globally. The objective of this thesis was to provide insights into the secondary metabolite pathways of this family. For this purpose, we used whole genome sequences of 23 different members of Ceratocystistidaceae. Our results showed that all of the genomes contained putative clusters containing a reducing and non-reducing type I PKS as well as a type III PKS. Phylogenetic analyses of non-reducing-PKS-I and also PKS-III suggested that these genes were already present in the ancestor of the Ceratocystidaceae. By contrast, the various reducing type I PKS-containing clusters identified in these genomes, appeared to have distinct origins during the evolution of this family. Although one of the identified clusters potentially allows for the production of melanin, their functional characterization will undoubtedly reveal many novel and important compounds implicated in the biology of the Ceratocystidaceae. We have also found two highly conserved nonribosomal peptide synthetase genes in all genomes of Ceratocystidaceae and their potential products were predicted. These findings help to better understanding of the diversity and evolution of NRPS biosynthesis pathways in this family. We further, optimized an Agrobacterium mediated transformation system for Ceratocystis. This will allow for the functional characterization of the genes and genetic elements underlying the biological properties of this important fungus and its relatives. The average ergosterol content of different genera of Ceratocystidaceae was different from each other. We also identified all possible terpenoid related genes and biosynthetic clusters in all genomes used in this study. We found a highly conserved terpenoid gene cluster containing some of the ergosterol biosynthetic genes in all genomes. An additional terpenoid gene cluster was also identified in all the Ceratocystidaceae with geranylgeranyl pyrophosphate as a core gene, which could be involve in diterpenoid production. The outcomes of this thesis shed light on our knowledge of secondary metabolite biosynthesis pathways in Ceratocystidaceae. Biochemistry, Genetics and Microbiology (BGM) PhD (Microbiology) Unrestricted Faculty of Natural and Agricultural Sciences 2024-09-19T12:36:33Z 2024-09-19T12:36:33Z 2020-04 2019-12 Thesis * A2020 http://hdl.handle.net/2263/98338 en © 2021 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Ceratocystidaceae Secondary metabolite pathways Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title | Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title_full | Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title_fullStr | Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title_full_unstemmed | Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title_short | Characterisation of secondary metabolite pathways in the Ceratocystidaceae |
| title_sort | characterisation of secondary metabolite pathways in the ceratocystidaceae |
| topic | UCTD Ceratocystidaceae Secondary metabolite pathways |
| url | http://hdl.handle.net/2263/98338 |