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Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi
Dissertation (MSc (Microbiology))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613708914524160 |
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| access_status_str | Open Access |
| author2 | Van den Berg, Noelani |
| author_browse | Van den Berg, Noelani |
| author_facet | Van den Berg, Noelani |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/98574 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:26.848Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/98574 Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi Van den Berg, Noelani katelyn.baird@fabi.up.ac.za Baird, Katelyn UCTD Sustainable Development Goals (SDGs) Necrosis and ethylene-inducing peptide 1-like protein (NLPs) Phytophthora cinnamomi Persea Americana Ethylene-induction Necrosis Dissertation (MSc (Microbiology))--University of Pretoria, 2024. This research began with identifying the total number of NLP-encoding genes from the P. cinnamomi transcriptome. Phylogenetic analysis was performed in conjunction with expression analysis from previously generated RNA-seq data of P. cinnamomi infection of two avocado rootstocks. Seven candidate genes were selected, based on the presence of the NLP motif, expression patterns of the genes and their phylogenetic similarities to known NLPs. The coding sequence for these candidates were confirmed with Sanger sequencing after cloning into E. coli. The resulting protein structures were visualised and analysed for similarity and an attempt was made to ascertain functionality, however, more research is needed to determine this. This study also investigated the genes responsible for ethylene production in plants to find a relationship between P. cinnamomi infection and ethylene induction. The same RNA-seq data was used to identify and assess the genes encoding the three enzymes for ethylene biosynthesis and their expression during infection. The findings from this study provide a solid framework for future research into the molecular mechanisms of action of the NLPs during infection. The individual functions of P. cinnamomi NLPs have yet to be discovered and the understanding of which NLPs act under which mechanisms is still a mystery. Biochemistry, Genetics and Microbiology (BGM) MSc (Microbiology) Restricted Faculty of Natural and Agricultural Sciences SDG-01: No poverty SDG-15: Life on land 2024-10-09T14:02:45Z 2024-10-09T14:02:45Z 2025-05 2024-07 Dissertation * A2025 http://hdl.handle.net/2263/98574 DOI: https://doi.org/10.25403/UPresearchdata.27193653.v1 https://doi.org/10.25403/UPresearchdata.27193653 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Necrosis and ethylene-inducing peptide 1-like protein (NLPs) Phytophthora cinnamomi Persea Americana Ethylene-induction Necrosis Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title | Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title_full | Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title_fullStr | Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title_full_unstemmed | Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title_short | Identification and molecular characterisation of the necrosis and ethylene-inducing peptide 1-like proteins (NLPs) of Phytophthora cinnamomi |
| title_sort | identification and molecular characterisation of the necrosis and ethylene inducing peptide 1 like proteins nlps of phytophthora cinnamomi |
| topic | UCTD Sustainable Development Goals (SDGs) Necrosis and ethylene-inducing peptide 1-like protein (NLPs) Phytophthora cinnamomi Persea Americana Ethylene-induction Necrosis |
| url | http://hdl.handle.net/2263/98574 https://doi.org/10.25403/UPresearchdata.27193653 |