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Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa
Dissertation (MSc (Microbiology))--University of Pretoria, 2024.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613526043918336 |
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| access_status_str | Open Access |
| author2 | Coetzee, Martin Petrus Albertus |
| author_browse | Coetzee, Martin Petrus Albertus |
| author_facet | Coetzee, Martin Petrus Albertus |
| collection | Thesis |
| dc_rights_str_mv | © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc (Microbiology))--University of Pretoria, 2024. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/99405 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:37:32.711Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/99405 Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa Coetzee, Martin Petrus Albertus mztapitasi@gmail.com Venter, S.N. (Stephanus Nicolaas) Steenkamp, Emma Theodora Tasiya, Taponeswa UCTD Sustainable Development Goals (SDGs) Rhizobia Acacia Genealogical concordance Bradyrhizobium Phylogeography Phylogeny Natural and agricultural sciences theses SDG-01 SDG-01: No poverty Natural and agricultural sciences theses SDG-02 SDG-02: Zero hunger Dissertation (MSc (Microbiology))--University of Pretoria, 2024. Chapter one of this dissertation provides a review of the literature pertaining to the bionts (i.e., rhizobia) and their role in both crop legumes and invasive Acacia. In the review, an emphasis was placed on Bradyrhizobium associating with invasive Acacia species in South Africa. The review also explored the current prospects for the taxonomy of rhizobia. The research presented in Chapter Two aimed to determine the taxonomic position of Bradyrhizobium species isolated from the root nodules of Acacia dealbata, Acacia decurrens and Acacia mearnsii in a previous study. This was achieved using various analyses typically used in studies focusing on bacterial systematics and delimiting species. These included delimited species based on the genealogical concordance of five housekeeping genes (i.e., 16S rRNA, dnaK, glnII, rpoB and recA), comparison with a multi-locus phylogenetic tree that was generated using the concatenated sequences of the individual genes, as well as calculating average nucleotide identities (ANIs) from whole genomes obtained in this study and those available from public databases. Conventional phenotypic tests were also done to associate phenotypic characteristics with the recognized species. In Chapter three, the research presented aimed to increase the knowledge of Bradyrhizobium diversity occurring in the root nodules of A. mearnsii, A. dealbata and A. decurrens growing in invasive settings. For this purpose, samples were obtained from sites in the KwaZulu Natal and Mpumalanga provinces of South Africa. Bacterial strains were isolated from the root nodules of the plants. The strains were identified through 16S rRNA sequencing analysis, and only strains identified as Bradyrhizobium using this approach were further used. The Bradyrhizobium strains were delineated into putative species using genealogical concordance analysis with sequences for the housekeeping genes gyrB, glnII, and rpoB. Multilocus sequence analysis was also conducted with the concatenated sequences for the rpoB and glnII genes to gain further support for the species identified. Lastly, phylogeographic inferences regarding the symbiotic properties were made based on phylogenetic analysis of sequences of the common nodulation gene nodA. Centre of Excellence in Tree Health Biotechnology (CTHB) Forestry and Agricultural Biotechnology Institute (FABI) Biochemistry, Genetics and Microbiology MSc (Microbiology) Unrestricted Faculty of Natural and Agricultural Sciences SDG-01: No poverty SDG-02: Zero Hunger 2024-11-26T11:18:30Z 2024-11-26T11:18:30Z 2025-04 2024-08 Dissertation * A2025 http://hdl.handle.net/2263/99405 DOI: https://doi.org/10.25403/UPresearchdata.27908076.v1 https://doi.org/10.25403/UPresearchdata.27908076 en © 2023 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | UCTD Sustainable Development Goals (SDGs) Rhizobia Acacia Genealogical concordance Bradyrhizobium Phylogeography Phylogeny Natural and agricultural sciences theses SDG-01 SDG-01: No poverty Natural and agricultural sciences theses SDG-02 SDG-02: Zero hunger Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title | Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title_full | Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title_fullStr | Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title_full_unstemmed | Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title_short | Diversity and description of Bradyrhizobium species associated with invasive Acacia species in South Africa |
| title_sort | diversity and description of bradyrhizobium species associated with invasive acacia species in south africa |
| topic | UCTD Sustainable Development Goals (SDGs) Rhizobia Acacia Genealogical concordance Bradyrhizobium Phylogeography Phylogeny Natural and agricultural sciences theses SDG-01 SDG-01: No poverty Natural and agricultural sciences theses SDG-02 SDG-02: Zero hunger |
| url | http://hdl.handle.net/2263/99405 https://doi.org/10.25403/UPresearchdata.27908076 |