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Microbial ecology of sulphide-producing bacteria in water cooling systems
Thesis (PhD)--University of Pretoria, 1992.
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| Format: | Thesis |
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University of Pretoria
2024
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| _version_ | 1867613487051571200 |
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| access_status_str | Open Access |
| author2 | Cloete, T.E. (Thomas Eugene), 1958- |
| author_browse | Cloete, T.E. (Thomas Eugene), 1958- |
| author_facet | Cloete, T.E. (Thomas Eugene), 1958- |
| collection | Thesis |
| dc_rights_str_mv | © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Thesis (PhD)--University of Pretoria, 1992. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/99532 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:36:55.449Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/99532 Microbial ecology of sulphide-producing bacteria in water cooling systems Cloete, T.E. (Thomas Eugene), 1958- Bruyn, Engela E. Microbial ecology Sulphide-producing bacteria Water cooling systems UCTD Thesis (PhD)--University of Pretoria, 1992. Microbiological induced corrosion (MIC) can result in damage and economic losses in various industries. Although the importance of sulphate-reducing bacteria (SRB) in MIC has been widely recognized for many years, there has been increasing evidence that other organisms in addition to SRB have been involved in the corrosion process. There is no information available regarding the ecology of SRB in industrial cooling water systems. The problem has been detecting and quantifying these organisms. The potential of using species specific fluorescent antibodies (FA) prepared against authentic SRB-strains for studying the ecology of SRB in industrial cooling water systems was investigated. Culture conditions influenced the expression of surface antigens, causing the antisera to be extremely specific and unsuitable for the identification of SRB enriched from industrial cooling water systems. Caution should be exercised when using FA for ecological studies. Different isolation media and carbon sources for the isolation and detection of SRB were evaluated. Iron sulphite (IS)-medium yielded the highest numbers when used to enumerate SRB from pure culture and industrial water samples. H2S-producing bacteria that utilized lactate, acetate, formate or palmitic acid as different carbon sources were also isolated from industrial water samples. Using IS-medium Shewanella putrefaciens was the dominant sulphide producing bacteria isolated from industrial cooling water systems. Corrosion studies indicated that S. putrefaciens could play an important role in microbially induced corrosion Since enumerating SRB by conventional methods is difficult, laborious and time consuming, the Malthus system, using conductance measurements, can be used for counting SRB cells in pure culture and thus for laboratory biocide evaluations. Microbiology and Plant Pathology PhD 2024-11-27T09:16:07Z 2024-11-27T09:16:07Z 21/11/04 1992 Thesis http://hdl.handle.net/2263/99532 en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Microbial ecology Sulphide-producing bacteria Water cooling systems UCTD Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title | Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title_full | Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title_fullStr | Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title_full_unstemmed | Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title_short | Microbial ecology of sulphide-producing bacteria in water cooling systems |
| title_sort | microbial ecology of sulphide producing bacteria in water cooling systems |
| topic | Microbial ecology Sulphide-producing bacteria Water cooling systems UCTD |
| url | http://hdl.handle.net/2263/99532 |