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Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity
Dissertation (MSc)--University of Pretoria, 1997.
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| Format: | Thesis |
| Language: | English |
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University of Pretoria
2024
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| _version_ | 1867613589557215232 |
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| access_status_str | Open Access |
| author2 | Verschoor, Jan Adrianus |
| author_browse | Verschoor, Jan Adrianus |
| author_facet | Verschoor, Jan Adrianus |
| collection | Thesis |
| dc_rights_str_mv | © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. |
| description | Dissertation (MSc)--University of Pretoria, 1997. |
| format | Thesis |
| id | oai:repository.up.ac.za:2263/99580 |
| institution | University of Pretoria (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:38:33.011Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UPSpace — University of Pretoria Institutional Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | University of Pretoria |
| publisherStr | University of Pretoria |
| record_format | dspace |
| source_str | UPSpace — University of Pretoria Institutional Repository |
| spelling | oai:repository.up.ac.za:2263/99580 Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity Verschoor, Jan Adrianus Gunter, Nicolaas Johannes Hybridoma formation Bacterial inducer entity UCTD Dissertation (MSc)--University of Pretoria, 1997. Spontaneous hybridoma formation was evaluated in this study. This phenomenon entails the treatment of mice with specific Haemophilus paragallinarum strains to induce the ability of murine splenocytes to fuse with tumour cells both in vitro and in vivo. It was descovered in the process of developing monoclonal antibodies against H. paragallinarum. The antigens on the relevant bacteria were further characterized ·to determine their chemical nature. Progress was also made in determining the optimal culture conditions for the production of bacteria that induce fusion. Two possible applications of the Haemophilus paragallinarum induced fusion were evaluated. The use of fusion inducing Haemophilus paragallinarum as an adjuvant for enhancing the local immune response and simultaneously acting as a fusing agent that simplifies the production of monoclonal antibodies against complex antigens. An animal model is described which enables the correlation of in vivo hybridoma formation with in vitro hybridoma forming ability. This provides evidence that reduced tumour growth rates observed in Haemophilus treated animals can be attributed to in vivo spontaneous hybridoma formation and that Haemophilus treatment therefore can have possible anticancer therapeutic use. Biochemistry MSc 2024-11-27T09:16:20Z 2024-11-27T09:16:20Z 21/11/16 1997 Dissertation http://hdl.handle.net/2263/99580 en © 2024 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. application/pdf University of Pretoria |
| spellingShingle | Hybridoma formation Bacterial inducer entity UCTD Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title | Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title_full | Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title_fullStr | Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title_full_unstemmed | Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title_short | Applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| title_sort | applications of spontaneous hybridoma formation and characterization of a bacterial inducer entity |
| topic | Hybridoma formation Bacterial inducer entity UCTD |
| url | http://hdl.handle.net/2263/99580 |