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The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).
Thesis (MSc)--Stellenbosch University, 2017.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2017
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| _version_ | 1867613867290394624 |
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| access_status_str | Open Access |
| author | Swart, Liezel Maria |
| author2 | Swart, Pieter |
| author_browse | Swart, Liezel Maria Swart, Pieter |
| author_facet | Swart, Pieter Swart, Liezel Maria |
| author_sort | Swart, Liezel Maria |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2017. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/101101 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:42:57.574Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/101101 The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). Swart, Liezel Maria Swart, Pieter Swart, Amanda C. Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Salsola tuberculata -- Namibia Salsola tuberculata -- Contraceptive properties Adrenal steriodogenesis and cytochrome enzymes Mitrochondrial electron transport chain isolation Karakul ewes -- Gestation Karakul sheep -- Abnormalities UCTD Thesis (MSc)--Stellenbosch University, 2017. ENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African shrub, Salsola tuberculataformis Botschantzev. . The development of a strategy to separate the R- and S-enantiomers of Compound A. . The isolation and purification of a highly purified substrate-free cytochrome P450 11β-hydroxylase (CYP11B1) from ovine adrenals. . The isolation of a partially purified mixture of the mitochondrial electron transport chain, adrenodoxin reductase and adrenodoxin from ovine adrenals. . The investigation into the mechanism of action of the R- and S-enantiomers of Compound A on the spectroscopic properties of substrate-free CYP11B1. . The investigation into the influence of the R- and S-enantiomers of Compound A on the mitochondrial electron transport chain by using cytochrome c as an electron acceptor. AFRIKAANSE OPSOMMNG: Hierdie studie beskryf: . Die sintese van ‘n rasemiese mengsel van Verbinding A (2-(4-asetoksifeniel)-2-chloro-N-metielammoniumchloried), ‘n sintetiese analoog van die aktiewe molecule wat in die Suider-Afrikaanse plant, Salsola tuberculataformis Botschantzev. . Die ontwikkeling van 'n strategie om van die R- en S-enantiomere van Verbinding A te skei. . Die isolering en suiwering van ‘n hoogs gesuiwerde, substraat-vrye sitochroom P450 11β-hidroksilase (CYP11B1) uit skaap-byniere. . Die isolering van ‘n gedeeltelike gesuiwerde mengsel van die mitokondriale elektrontransportketting, adrenodoksienreduktase en adrenodoksien uit skaapbyniere. . ‘n Ondersoek na die meganisme van werking van die R- en S-enantiomere van Verbinding A op die spektroskopiese eienskappe van ‘n gesuiwerde substraat-vrye CYP11B1. . ‘n Ondersoek na die invloed van die R- en S-enantiomere van Verbinding A op die mitokondriale elektrontransportketting met behulp van sitochroom c as 'n elektronakseptor. 2017-02-20T14:51:23Z 2017-03-29T12:08:15Z 2017-02-20T14:51:23Z 2017-03-29T12:08:15Z 2017-03 Thesis http://hdl.handle.net/10019.1/101101 en_ZA Stellenbosch University 134 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Salsola tuberculata -- Namibia Salsola tuberculata -- Contraceptive properties Adrenal steriodogenesis and cytochrome enzymes Mitrochondrial electron transport chain isolation Karakul ewes -- Gestation Karakul sheep -- Abnormalities UCTD Swart, Liezel Maria The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title | The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title_full | The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title_fullStr | The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title_full_unstemmed | The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title_short | The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A). |
| title_sort | biochemical study of the r and s enantiomers of 2 4 acetoxyphenyl 2 chloro nmethylethylammonium chloride compound a |
| topic | Salsola tuberculata -- Namibia Salsola tuberculata -- Contraceptive properties Adrenal steriodogenesis and cytochrome enzymes Mitrochondrial electron transport chain isolation Karakul ewes -- Gestation Karakul sheep -- Abnormalities UCTD |
| url | http://hdl.handle.net/10019.1/101101 |
| work_keys_str_mv | AT swartliezelmaria thebiochemicalstudyoftherandsenantiomersof24acetoxyphenyl2chloronmethylethylammoniumchloridecompounda AT swartliezelmaria biochemicalstudyoftherandsenantiomersof24acetoxyphenyl2chloronmethylethylammoniumchloridecompounda |