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The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat
Thesis (PhD (Biochemistry))--Stellenbosch University, 2008.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2008
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| _version_ | 1867613936024551424 |
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| access_status_str | Open Access |
| author | Storbeck, Karl-Heinz |
| author2 | Swart, P. |
| author_browse | Storbeck, Karl-Heinz Swart, P. |
| author_facet | Swart, P. Storbeck, Karl-Heinz |
| author_sort | Storbeck, Karl-Heinz |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (PhD (Biochemistry))--Stellenbosch University, 2008. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/1101 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:03.396Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/1101 The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat Storbeck, Karl-Heinz Swart, P. Swart, Amanda C. Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Cytochrome P450 CYP17 expression Hydrocortisone Angora goats -- Physiology Adrenocortical hormones Dissertations -- Biochemistry Theses -- Biochemistry Thesis (PhD (Biochemistry))--Stellenbosch University, 2008. This study describes: • the cloning and sequencing of cytochrome P450 17 -hydroxylase/17,20 lyase (CYP17), 3 -hydroxysteroid dehydrogenase (3 HSD) and cytochrome b5 from the South African Angora goat; • the identification of two CYP17 genes encoding two unique CYP17 isoforms in the Angora goat; • the development of a UPLC-APCI-LC method for the separation and quantification of seven adrenal steroids; • the characterisation of the enzymatic activity of the two Angora CYP17 isoforms expressed in non-steroidogenic COS-1 cells. The Km and Vvalues for the metabolism of pregnenolone and progesterone were determined; • the development of a rapid and accurate real-time PCR genotyping test for CYP17 in Angora goats. Three unique genotypes were identified; • the determination of blood cortisol levels upon the stimulation of the HPAaxis by intravenous insulin injection in the three Angora goat genotypes. Doctoral 2008-11-17T12:08:09Z 2010-06-01T08:12:27Z 2008-11-17T12:08:09Z 2010-06-01T08:12:27Z 2008-12 Thesis http://hdl.handle.net/10019.1/1101 en Stellenbosch University application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Cytochrome P450 CYP17 expression Hydrocortisone Angora goats -- Physiology Adrenocortical hormones Dissertations -- Biochemistry Theses -- Biochemistry Storbeck, Karl-Heinz The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title | The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title_full | The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title_fullStr | The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title_full_unstemmed | The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title_short | The influence of dual CYP17 expression on adrenal steroidogenesis in the South African Angora Goat |
| title_sort | influence of dual cyp17 expression on adrenal steroidogenesis in the south african angora goat |
| topic | Cytochrome P450 CYP17 expression Hydrocortisone Angora goats -- Physiology Adrenocortical hormones Dissertations -- Biochemistry Theses -- Biochemistry |
| url | http://hdl.handle.net/10019.1/1101 |
| work_keys_str_mv | AT storbeckkarlheinz theinfluenceofdualcyp17expressiononadrenalsteroidogenesisinthesouthafricanangoragoat AT storbeckkarlheinz influenceofdualcyp17expressiononadrenalsteroidogenesisinthesouthafricanangoragoat |