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Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation
Thesis (MSc)--Stellenbosch University, 2021.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2021
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| _version_ | 1867613747408797697 |
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| access_status_str | Open Access |
| author | Sibariboyi, Sinothando |
| author2 | Klumperman, Bert |
| author_browse | Klumperman, Bert Sibariboyi, Sinothando |
| author_facet | Klumperman, Bert Sibariboyi, Sinothando |
| author_sort | Sibariboyi, Sinothando |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2021. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/110339 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:41:01.634Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/110339 Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation Sibariboyi, Sinothando Klumperman, Bert Pfukwa, Rueben Stellenbosch University. Faculty of Science. Dept. of Chemistry and Polymer Science. Polyzwitterions Copolymers Water-soluble polymers Nanostructured materials UCTD Thesis (MSc)--Stellenbosch University, 2021. ENGLISH ABSTRACT: In this study, low molecular weight poly(styrene-co-maleic anhydride) (SMAnh) copolymer variants were used to synthesize a novel class of zwitterionic copolymers with varying anionic functionalities (i.e., sulfonate, carboxylate, and phosphonate). Characterization of the zwitterionic copolymers was done via ATR-FTIR and NMR spectroscopic analyses. The solubility of the zwitterionic copolymers was initially evaluated in water to determine their feasibility for application in lipid solubilization study. At ambient temperature, only the carboxybetaine and phosphobetaine derivatives were water-soluble, while the sulfobetaine derivatives were insoluble in water. Consequently, the lipid solubilization efficiency of the water-soluble zwitterionic copolymer derivatives was evaluated via turbidimetric and DLS analyses. All zwitterionic copolymers successfully solubilized DMPC multilamellar vesicles into nanodiscs of 6 – 20 nm in diameter. Moreover, the resultant nanodiscs displayed much-improved pH stability (4 – 10) and tolerance toward increased salt concentration of MgCl2 (up to 50 mM) compared to well-known commercial SMA copolymer. Additionally, the zwitterionic copolymers were able to isolate ZipA protein in high purity but at lower concentrations compared to SMA. Overall, the derivative with propyl carboxylic acid substituent (PCZ2K) exhibited the most promising results and therefore requires further exploration. As a follow up to the water-insoluble sulfobetaine derivatives, a brief thermoresponsive study was conducted. Successful dissolution of sulfobetaines was observed upon application of heat and increased salt concentration, which aided in the dissociation of the dipole-dipole interactions. This demonstrated that the sulfobetaines exhibited upper critical solution temperature (UCST) behavior. AFRIKAANSE OPSOMMING: In hierdie studie is lae-molekulêre gewig poli (stireen-ko-maleïn-anhidried) (SMAnh) kopolymeer variante gebruik om 'n nuwe klas zwitterioniese kopolimere met verskillende anioniese funksies (d.w.s. sulfonaat, karboksilaat en fosfonaat) te sintetiseer. Karakterisering van zwitterioniese kopolimere is gedoen deur middel van ATR-FTIR- en NMR-spektroskopiese ontledings. Die oplosbaarheid van die zwitterioniese kopolimere is aanvanklik in water geëvalueer om die haalbaarheid daarvan vir toepassing in lipiedoplosbare studie te bepaal. By kamertemperatuur was slegs die karboksbetaïen- en fosfobetaïen-derivate wateroplosbaar, terwyl die sulfobetaïen-derivate onoplosbaar gebly het. Gevolglik is die lipied oplosbaarheidseffektiwiteit van die wateroplosbare zwitterioniese kopolimeer afgeleides geëvalueer deur middel van turbidimetriese en DLS-ontledings. Alle zwitterioniese kopolimeer het DMPC-lipiede suksesvol opgelos in nanoskyfies van 5-20 nm in deursnee. Boonop vertoon die nanoskyfies wat deur die zwitterioniese kopolimeer geproduseer word, baie beter pH-stabiliteit (5-10) en verdraagsaamheid teenoor verhoogde soutkonsentrasie van MgCl2 (tot 50 mM) in vergelyking met bekende kommersiële die SMA2000P-kopolimeer. Daarbenewens kon die zwitterioniese kopolimere 'n ZipA-model proteïen suiwer met 'n hoë suiwerheid, maar laer konsentrasies in vergelyking met SMA2000P. Oor die algemeen lyk die afgeleide met propielkarboksielsuur substituent (PCZ2K) die belowendste vir verdere ondersoek. As opvolg van die wateroplosbare sulfobetaïen-afgeleides is 'n kort termoresponsiewe studie gedoen. Suksesvolle oplossing van sulfobetaïenes is waargeneem met 'n toename in temperatuur en soutkonsentrasie wat die dissosiasie van die dipool-dipool-interaksies aangehelp het. Dit was 'n bewys dat die sulfobetaïenes UCST-gedrag vertoon. Masters 2021-04-22T10:51:55Z 2023-05-03T03:00:07Z 2021-03 Thesis http://hdl.handle.net/10019.1/110339 en Stellenbosch University xiii, 94 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Polyzwitterions Copolymers Water-soluble polymers Nanostructured materials UCTD Sibariboyi, Sinothando Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title | Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title_full | Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title_fullStr | Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title_full_unstemmed | Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title_short | Synthesis of poly (styrene-co-maleic anhydride)-based zwitterionic copolymers for nanodisc formation |
| title_sort | synthesis of poly styrene co maleic anhydride based zwitterionic copolymers for nanodisc formation |
| topic | Polyzwitterions Copolymers Water-soluble polymers Nanostructured materials UCTD |
| url | http://hdl.handle.net/10019.1/110339 |
| work_keys_str_mv | AT sibariboyisinothando synthesisofpolystyrenecomaleicanhydridebasedzwitterioniccopolymersfornanodiscformation |