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Towards automated detection of dicentric chromosomes in metaphase images
Thesis (MSc)--Stellenbosch University, 2021.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2021
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| _version_ | 1867613969283284992 |
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| access_status_str | Open Access |
| author | Galloway, Sarah |
| author2 | Coetzer, Johannes |
| author_browse | Coetzer, Johannes Galloway, Sarah |
| author_facet | Coetzer, Johannes Galloway, Sarah |
| author_sort | Galloway, Sarah |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2021. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/110495 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:44:35.400Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/110495 Towards automated detection of dicentric chromosomes in metaphase images Galloway, Sarah Coetzer, Johannes Muller, N. Stellenbosch University. Faculty of Science. Dept. of Mathematical Sciences. Division Applied Mathematics. Chromosomes in metaphase images Chromosome abnormalities -- Detection Digital image processing Dicentric chromosomes Metaphase images UCTD Thesis (MSc)--Stellenbosch University, 2021. ENGLISH ABSTRACT: The aim of the proposed research is to investigate methods to identify objects of interest and classify dicentric and normal chromosomes in metaphase images using suitable digital image processing techniques. Dicentric chromosomes are abnormal chromosomes with two centromeres (instead of one) created by a variety of processes, including irradiation. When a chromosome is exposed to radiation, two chromosome segments, each with a centromere may join together resulting in a dicentric chromosome. An acentric fragment, i.e. a partial chromosome with no centromere, is also formed. The first stage of the proposed system is geared towards the detection of objects of interest, i.e. isolated normal and isolated dicentric chromosomes, as well as acentric fragments and clusters of overlapping chromosomes. The last stage of the proposed system is geared towards the classification of isolated chromosomes as either normal or dicentric. The proposed system automatically detects objects of interest not associated with dirt. The classification of the aforementioned objects into isolated and clustered chromosomes, as well as acentric fragments, is conducted manually, while the automation of this stage is reserved for future work. The proposed system subsequently automatically categorises isolated chromosomes as either normal or dicentric. It is demonstrated that the system correctly detects and classifies a significant number of the aforementioned chromosomes within metaphase images provided by iThemba LABS. AFRIKAANSE OPSOMMING: Die doel van die voorgestelde navorsing is om metodes te ondersoek wat voorwerpe van belang in metafase-beelde identifiseer en disentriese en normale chromosome met behulp van geskikte beeldverwerkingstegnieke klassifiseer. Disentriese chromosome is abnormale chromosome met twee sentromere (in plaas van een) wat deur verskeie prosesse, insluitende bestraling, geskep word. Wanneer ’n chromosoom aan bestraling blootgestel word, kan twee chromosoomsegmente, elk met ’n sentromeer, saamgevoeg word wat ’n disentriese chromosoom tot gevolg het. ’n Asentriese fragment, dit wil sˆe ’n gedeeltelike chromosoom sonder ’n sentromeer, word ook gevorm. Die eerste fase van die voorgestelde stelsel is op die opsporing van voorwerpe van belang gerig, dit wil sˆe ge¨ısoleerde normale en ge¨ısoleerde disentriese chromosome, sowel as asentriese fragmente en groepe van oorvleulende chromosome. Die laaste fase van die voorgestelde stelsel is op die klassifikasie van ge¨ısoleerde chromosome as normaal of disentries gerig. Die voorgestelde stelsel bespeur outomaties voorwerpe van belang wat nie met vuilheid verband hou nie. Die klassifikasie van bogenoemde voorwerpe as ge¨ısoleerde en gegroepeerde chromosome, asook asentriese fragmente, word met die hand gedoen, terwyl die outomatisering van hierdie fase vir toekomstige werk gereserveer is. Die voorgestelde stelsel kategoriseer vervolgens ge¨ısoleerde chromosome outomaties as normaal of disentries. Daar word aangetoon dat die stelsel ’n beduidende aantal van bogenoemde chromosome korrek opspoor en klassifiseer binne die metafase-beelde wat deur iThemba LABS verskaf is. Masters 2021-05-24T12:54:41Z 2021-05-24T12:54:41Z 2021-03 Thesis http://hdl.handle.net/10019.1/110495 en_ZA Stellenbosch University 96 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Chromosomes in metaphase images Chromosome abnormalities -- Detection Digital image processing Dicentric chromosomes Metaphase images UCTD Galloway, Sarah Towards automated detection of dicentric chromosomes in metaphase images |
| title | Towards automated detection of dicentric chromosomes in metaphase images |
| title_full | Towards automated detection of dicentric chromosomes in metaphase images |
| title_fullStr | Towards automated detection of dicentric chromosomes in metaphase images |
| title_full_unstemmed | Towards automated detection of dicentric chromosomes in metaphase images |
| title_short | Towards automated detection of dicentric chromosomes in metaphase images |
| title_sort | towards automated detection of dicentric chromosomes in metaphase images |
| topic | Chromosomes in metaphase images Chromosome abnormalities -- Detection Digital image processing Dicentric chromosomes Metaphase images UCTD |
| url | http://hdl.handle.net/10019.1/110495 |
| work_keys_str_mv | AT gallowaysarah towardsautomateddetectionofdicentricchromosomesinmetaphaseimages |