Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses
Thesis (MMed)--Stellenbosch University, 2021.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
| Published: |
Stellenbosch : Stellenbosch University
2021
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613882917322752 |
|---|---|
| access_status_str | Open Access |
| author | Mahlakwane, Kamela L. |
| author2 | Van Zyl, Gert |
| author_browse | Mahlakwane, Kamela L. Van Zyl, Gert |
| author_facet | Van Zyl, Gert Mahlakwane, Kamela L. |
| author_sort | Mahlakwane, Kamela L. |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MMed)--Stellenbosch University, 2021. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/123660 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:43:12.690Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/123660 Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses Mahlakwane, Kamela L. Van Zyl, Gert Preiser, Wolfgang Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Pathology: Medical Virology. HIV -- Infant -- Diagnosis UCTD HIV-positive children Antiretroviral drugs Diagnosis, Laboratory Polymerase chain reaction -- Diagnostic use Thesis (MMed)--Stellenbosch University, 2021. Background The early diagnosis and confirmation of HIV infection in newborns is crucial for expedited antiretroviral therapy initiation. Confirmatory testing must be done for all children with a reactive HIV PCR result. There is no comprehensive data on confirmatory testing and rejection of HIV PCR test requests at National Health Laboratory Service laboratories. Aim and objectives To assess relevant measures for routine infant HIV PCR testing: rate of rejected test requests, turnaround time, and rate of confirmatory testing. Method A retrospective review was performed on the laboratory-based data of all HIV PCR tests that were performed on children ≤24 months old (n=43,346), and data of rejected HIV PCR requests (n=1,479) over a two-year period (2017-2019). These data were extracted from the laboratory information system. Data were analyzed from sample collection to release of results, assessing the TAT and follow-up patterns. Results The proportion of HIV PCR requests that were rejected was 3.3%, of which 83.9% were rejected for various pre-analytical reasons. The majority of test results (89.2%) met the required 96-hour TAT. Of the reactive initial test results, 53.5% had a follow-up sample sent, of which 93.1% were positive on follow-up. Of the initial indeterminate results, 74.7% were negative on follow-up. Conclusion A significant proportion of HIV PCR requests were rejected for various pre-analytical reasons. The high number of initial reactive tests, without evidence of follow-up, may suggest that a shorter TAT would be required to allow confirmatory testing, before children are discharged. AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar Masters 2021-07-13T09:25:08Z 2021-12-22T14:14:33Z 2021-07-13T09:25:08Z 2021-12-22T14:14:33Z 2021-12 Thesis http://hdl.handle.net/10019.1/123660 en_ZA Stellenbosch University 34 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | HIV -- Infant -- Diagnosis UCTD HIV-positive children Antiretroviral drugs Diagnosis, Laboratory Polymerase chain reaction -- Diagnostic use Mahlakwane, Kamela L. Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title | Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title_full | Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title_fullStr | Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title_full_unstemmed | Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title_short | Delays in HIV-1 infant PCR testing may leave children without confirmed diagnoses |
| title_sort | delays in hiv 1 infant pcr testing may leave children without confirmed diagnoses |
| topic | HIV -- Infant -- Diagnosis UCTD HIV-positive children Antiretroviral drugs Diagnosis, Laboratory Polymerase chain reaction -- Diagnostic use |
| url | http://hdl.handle.net/10019.1/123660 |
| work_keys_str_mv | AT mahlakwanekamelal delaysinhiv1infantpcrtestingmayleavechildrenwithoutconfirmeddiagnoses |