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The Design and development towards a biosensor for the detection of Bovine Tuberculosis
Thesis (MEng)--Stellenbosch University, 2021.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2021
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| _version_ | 1867614013838327808 |
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| access_status_str | Open Access |
| author | Hess, Ronja |
| author2 | Perold, Willem |
| author_browse | Hess, Ronja Perold, Willem |
| author_facet | Perold, Willem Hess, Ronja |
| author_sort | Hess, Ronja |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MEng)--Stellenbosch University, 2021. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/123704 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:45:17.761Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/123704 The Design and development towards a biosensor for the detection of Bovine Tuberculosis Hess, Ronja Perold, Willem Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering. UCTD Biosensors -- Design and Development Bovine Tuberculosis -- Detection Communicable diseases Surface plasmon resonance Thesis (MEng)--Stellenbosch University, 2021. ENGLISH ABSTRACT: Bovine Tuberculosis (bTB) is a contagious disease often found in buffaloes and cattle. The disease can affect various mammals and poses a huge socioeconomic risk. Current diagnostic methods require highly skilled personnel and cannot be done in the field. Hence, the development of a biosensor to detect bTB is proposed. This thesis details the design of a system towards the development of a biosensor to detect bTB in buffalo. A literature study to investigate biosensors was conducted and Surface Plasmon Resonance (SPR) was selected as the biosensor type. SPR is an optical phenomenon that can be implemented to monitor binding and unbinding of biological components. For this project various parts of an SPR system were designed, manufactured and tested. SPR chips with a 50 nm thick gold substrate were manufactured. Various optical setup configurations were tested and prism types were investigated. The Infiniter VLM-635/650-03 laser was selected and a power supply system was designed and produced. The SPR phenomenon was observed using these components. The microfluidic system that was developed allowed for the introduction of the sample to the gold layer at low velocity. The prototype was flexible, to allow for adjustments during development and testing phase. Two different immobilisation methods were attempted and various verification methods were implemented, but the results were inconclusive. Software to plot the intensity profiles of the SPR system was developed. The prototype was tested with bovine serum albumin and at a concentration of 10 mg·ml−1 a change in the intensity minimum of the signal could be observed with the naked eye. Even though the system needs refinement and further development, it is concluded that an SPR based biosensor is a viable solution for the in the field detection of bTB. AFRIKAANSE OPSOMMING: Beestuberkulose (bTB) is ’n aansteeklike siekte wat dikwels by buffels en beeste voorkom. Die siekte kan verskillende soogdiere aantas en hou ’n groot sosio-ekonomiese risiko in. Huidige diagnostiese metodes benodig hoogs bekwame personeel en kan nie in die veld gedoen word nie. Daarom word die ontwikkeling van ’n biosensor vir die opsporing van bTB voorgestel. Hierdie tesis beskryf die ontwerp van ’n stelsel vir die ontwikkeling van ’n biosensor om tuberkulose by beeste op te spoor. ’n Literatuurstudie om biosensors te ondersoek is uitgevoer en ‘n oppervlak plasmon resonansie biosensor is gekies vir die pro- jek. Oppervlak Plasmon Resonansie (OPR) is ’n optiese verskynsel wat ge ̈ımplementeer kan word om binding en ontbinding van biologiese komponente te monitor. Vir hierdie projek is verskillende dele van ’n OPR-stelsel ontwerp, vervaardig en getoets. OPR -skyfies met ’n 50 nm dik goue substraat is vervaardig. Verskeie optiese opstellings is getoets en prisma-soorte is ondersoek. Die Infiniter VLM-635/650-03 laser is gekies en ’n kragtoevoerstelsel is ontwerp en vervaardig. Die OPR-verskynsel is waargeneem deur die gebruik van hierdie komponente. Die mikroflu ̈ıdiese stelsel wat ontwikkel is, het toegelaat dat die monster teen ’n lae snelheid met die goue laag kontak maak. Die prototipe was verstelbaar om aanpassings tydens die ontwikkelings- en toetsfase moontlik te maak. Daar is twee verskillende immobilisasie-metodes getoets en verskillende veri- fikasiemetodes is ge ̈ımplementeer, maar die resultate was onduidelik. Sagteware om die intensiteitsprofiele van die OPR-stelsel grafies voor te stel, is ontwikkel. Die prototipe is met beesserumalbumien getoets en by ’n konsentrasie van 10 mg·ml−1 kon ’n verandering in die intensiteit minimum in die sein met die blote oog waargeneem word. Alhoewel die stelsel verfyning en verdere ontwikkeling nodig het word dit afgelei dat ’n OPR-gebaseerde biosensor vir die opsporing van bTB in die veld ’n uitvoerbare oplossing is. Masters 2021-10-12T07:57:10Z 2021-12-22T14:16:49Z 2021-10-12T07:57:10Z 2021-12-22T14:16:49Z 2021-12 Thesis http://hdl.handle.net/10019.1/123704 en_ZA Stellenbosch University 176 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | UCTD Biosensors -- Design and Development Bovine Tuberculosis -- Detection Communicable diseases Surface plasmon resonance Hess, Ronja The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title | The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title_full | The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title_fullStr | The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title_full_unstemmed | The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title_short | The Design and development towards a biosensor for the detection of Bovine Tuberculosis |
| title_sort | design and development towards a biosensor for the detection of bovine tuberculosis |
| topic | UCTD Biosensors -- Design and Development Bovine Tuberculosis -- Detection Communicable diseases Surface plasmon resonance |
| url | http://hdl.handle.net/10019.1/123704 |
| work_keys_str_mv | AT hessronja thedesignanddevelopmenttowardsabiosensorforthedetectionofbovinetuberculosis AT hessronja designanddevelopmenttowardsabiosensorforthedetectionofbovinetuberculosis |