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Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth
Thesis (MSc)--Stellenbosch University, 2022.
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| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2022
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| _version_ | 1867613783293165568 |
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| access_status_str | Open Access |
| author | Visagie, Aiden Weidner |
| author2 | Jacobs, Karin |
| author_browse | Jacobs, Karin Visagie, Aiden Weidner |
| author_facet | Jacobs, Karin Visagie, Aiden Weidner |
| author_sort | Visagie, Aiden Weidner |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2022. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/126286 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:37.777Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/126286 Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth Visagie, Aiden Weidner Jacobs, Karin Visagie, Cobus Meyer Stellenbosch University. Faculty of Science. Dept. of Microbiology. Penicillium Wheat -- Breeding Soil chemistry Growth (Plants) -- Effect of stress on Wheat -- Growth -- Effect of chemicals on Plant-soil relationships Penicillium -- Genetic aspects Fungi UCTD Thesis (MSc)--Stellenbosch University, 2022. ENGLISH ABSTRACT: The role of Penicilium in agricultural soils is undeniable, with fungi of this genus having a large role in nutrient cycling, as well as potentially forming symbiotic associations or causing post-harvest decay. However, the effect which agriculutrual practices may have on these fungi is not well understood, with several conflicting results reported in literature. The penchant of the genus Penicillium to promote plant growth, particularly of wheat, has been established, but few of these environments have been explored for potentially useful plant growth-promoting Penicillium. The fynbos biome is also rich in Penicillium, as has been seen in recent years, with many new species being described from these environments. The diversity of Penicillium in ex-fynbos soils, or fynbos converted to farmLand, has not been investigated in depth. In this thesis, we explored Penicillium diversity in ex-fynbos wheat-growing agricultural soil samples. These soil samples differed in terms of location, crop rotation applied, and time of collection. Soil chemical parameters were also determined, and the relationships between the sampling time, crop rotation, location and various soil chemical characteristics were investigated. A total of 632 fungal strains were isolated belonging to Penicillium (n = 574), Aspergillus (n = 43) and Talaromyces (n = 15). They represent 28 Penicillium, 8 Aspergillus and 2 Talaromyces species. This study identified and described two new species as Aspergillus tygerhoekense prov. nom. and Penicillium shaneanum prov. nom. based on multigene phylogenies and morphological characterisation. The ability of isolated species to solubilize phosphate was also investigated. Several Penicillium species had strong solubilization activity and in a potting trial setting, the ability of five strains to promote plant growth was investigated. Three of the five strains showed the ability to increase root formation in the wheat seedlings tested. The effect of crop rotations have on community composition were found to be minimal, whilst location and sampling time both had significant impacts. Many phosphate-solubilizing fungi were isolated, showing these environments may hold potentially useful organisms. AFRIKAANSE OPSOMMING: Die neiging van die genus Penicillium om plantegroei te bevorder, veral in koring, is bevestig, maar voordelige Penicillium spesies in koring-landbouomgewings moet nog bestudeer word. Verder die fynbosbioom is ook ryk aan Penicillium, soos die afgelope jare gesien is, met baie nuwe spesies wat uit hierdie omgewings beskryf word. Die diversiteit van Penicillium in historiese-fynbosgronde, of fynbos wat na landbougrond omgeskakel is, is nog nie in diepte ondersoek nie. In hierdie proefskrif is die Penicillium diversiteit ondersoek binne histories fynbos areas wat huidiglik vir koringverbouing gebruik word. Hierdie grondmonsters het van mekaar verskil in terme van ligging, wisselbou toepassings en tyd van versameling. Die verskille tussen die monsternemingstyd, wisselbou, ligging en verskeie grondchemiese eienskappe is ondersoek. 'n Totaal van 632 swamstamme is geïsoleer wat aan Penicillium (n = 574), Aspergillus (n = 43) en Talaromyces (n = 15) behoort, waarvan 28 Penicillium, 8 Aspergillus en 2 Talaromyces spesies geindentifiseer kon word. Tydens hierdie studie was twee van die geïsoleerde spesies, Aspergillus tygerhoekense prov. nom. en Penicillium shaneanum prov. nom. beskryf as nuwe spesies deur gebruik te maak van beide multigeen filogenetiese analieses en morfologiese karakterisering. Die vermoë van geselekteerde fungi om fosfate te miniraliseer is ook ondersoek. Verskeie van die geïsoleerde Penicillium het hoë vlakke van hierdie aktiwiteit getoon. Verder in 'n potproefeksperiment is die vermoë van vyf spesies om plantgroei te bevorder ondersoek. Drie van die vyf stamme het die vermoë getoon om wortelvorming te verhoog in die koringsaailinge. Die effek van wisselboupraktyke wat waargeneem is, was minimaal, terwyl ligging en steekproeftyd beide sleutel was tot die faktore wat ondersoek is. Baie swamme met fosfaatoplosssing aktiwiteit is geïsoleer, wat aandui dat hierdie omgewings moontlik voordelige organismes kan bevat vir landbou aktiwiteite. Masters 2022-10-18T10:29:57Z 2023-01-23T06:50:28Z 2023-05-03T03:00:08Z 2022-12 Thesis http://hdl.handle.net/10019.1/126286 en_ZA Stellenbosch University 157 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Penicillium Wheat -- Breeding Soil chemistry Growth (Plants) -- Effect of stress on Wheat -- Growth -- Effect of chemicals on Plant-soil relationships Penicillium -- Genetic aspects Fungi UCTD Visagie, Aiden Weidner Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title | Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title_full | Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title_fullStr | Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title_full_unstemmed | Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title_short | Isolation and identification of Penicillium spp. from wheat-growing agricultural soils and their potential to promote plant growth |
| title_sort | isolation and identification of penicillium spp from wheat growing agricultural soils and their potential to promote plant growth |
| topic | Penicillium Wheat -- Breeding Soil chemistry Growth (Plants) -- Effect of stress on Wheat -- Growth -- Effect of chemicals on Plant-soil relationships Penicillium -- Genetic aspects Fungi UCTD |
| url | http://hdl.handle.net/10019.1/126286 |
| work_keys_str_mv | AT visagieaidenweidner isolationandidentificationofpenicilliumsppfromwheatgrowingagriculturalsoilsandtheirpotentialtopromoteplantgrowth |