Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Towards a paper-based electrochemical glucose biosensor
Thesis (MEng)--Stellenbosch University, 2023.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA en_ZA |
| Published: |
Stellenbosch : Stellenbosch University,
2023
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613891030155264 |
|---|---|
| access_status_str | Open Access |
| author | Francois, Snyders |
| author2 | Booysen, Thinus |
| author_browse | Booysen, Thinus Francois, Snyders |
| author_facet | Booysen, Thinus Francois, Snyders |
| author_sort | Francois, Snyders |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University, |
| description | Thesis (MEng)--Stellenbosch University, 2023. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/127082 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA en_ZA |
| last_indexed | 2026-06-10T12:43:20.403Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Stellenbosch : Stellenbosch University, |
| publisherStr | Stellenbosch : Stellenbosch University, |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/127082 Towards a paper-based electrochemical glucose biosensor Francois, Snyders Booysen, Thinus Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering. Blood glucose monitoring Neonatology Biosensors Electrochemical sensors Thesis (MEng)--Stellenbosch University, 2023. ENDGLISH ABSTRACT: Glucose homeostasis forms a critical part of the development of a healthy neonate. The complex adaptation from fetal to neonatal life requires well-coordinated hormonal and metabolic adaptive changes to maintain glucose homeostasis. At-risk infants face high morbidity- and mortality risks and are also at larger risk of developing glucose metabolism disorders during their first week of e xtra-uterine l ife. Hyperglycemia i s a common glucose metabolism disorder developed in at-risk infants and is frequently accompanied by a urinary loss of glucose. The concentration of glucose excreted through neonatal urine can give an indication of the hyperglycemic state of the neonate. Glycosuria monitoring can therefore be used as an additional screening method to detect hyperglycemia - an alternative to frequently measuring blood glucose. This project aimed to develop a paper-based glucose sensor that uses electrochemistry and glucose-specific e nzyme r eactions t o d etect g lucose c oncentrations relevant to neonatal hyperglycemic glycosuria thresholds in sample solutions. The sensor was manufactured on photo paper in the form of a printed three-electrode sensor using a desktop ink-jet printer as the manufacturing tool. A compatible PEDOT:PSS ink dispersion was formulated for this printer by optimising the concentration ratios of its constituents. Thin polymer films were p rinted i n s uccessive l ayers a nd were treated with organic solvents and annealing procedures to achieve optimal levels of film conductivity. An enzyme solution containing glucose oxidase enzymes and artificial mediators was prepared and validated for its ability to oxidise glucose. By combining the printed electrodes with the prepared enzyme solution, cyclical voltammetric methods were applied with the addition of glucose to realise an electrochemical glucose biosensor. Glucose concentrations ranging from 0 32 mg=ml were tested using the fabricated sensors. The sensor proved its capability by consistently detecting all glucose concentrations relevant to hyperglycemic glycosuria thresholds in neonatal urine. A shortcoming of the proposed sensor solution included the incapability to differentiate between glucose concentrations of 4 16 mg=ml. AFRIKAANS OPSOMMING: Glukose homeostase speel ’n kritieke rol in die ontwikkeling van ’n gesonde neonaat. Die komplekse verandering vanaf fetale- na neonatale lewe verg goed gekoördineerde hormonale- en metabolise aanpassings om glukose homeostase te handhaaf. Gesondheids riskante babas staar hoë morbiditeits en sterflikheids risiko’s in die gesig en is ook in groter gevaar om metaboliese glukose versteurings in hul eerste week na geboorte te ontwikkel. Hiperglukemie is ’n algemene metaboliese glukose versteuring wat in gesondheids riskante babas ontwikkel. Hiperglukemie gaan dikwels gepaard met ’n glukose verlies in urine. Die konsentrasie van glukose wat in neonatale urine afgeskei word kan aanduiding gee van die hiperglukemiese staat van die neonaat. Urine glukose monitering kan dus gebruik word as ’n addisionele iftingsinstrument vir die bespeuring van hiperglukemie, alternatief tot gereelde bloed glukose metings. Hierdie projek het beoog om ’n papier-gebaseerde glukose sensor te vervaardig wat gebruik maak van elektrochemiese tegnieke en glukose-spesifieke e n sieme o m glukose konsentrasies toepaslik tot neonatale hiperglukemiese urine glukose vlakke in oplossings te meet. Die sensor was in die vorm van ’n drie-elektrode sensor geskep op foto papier deur gebruik te maak van ’n inkjet drukker as die vervaardigings werktuig. ’n Aansluitbare PEDOT:PSS ink was vir hierdie inkjet drukker ontwikkel deur middel van die konsentrasie samestelling van die ink middels te optimaliseer. Dun polimeer films w a s g e druk i n o p eenvolgende l a e e n b e handel m e t o r ganiese o p losmiddels en bak prosedures om optimale geleidingsvermoë in die films t e v e rkry. ’ n Ensiem oplossing wat glukose oksidase ensieme en kunsmatige bemiddelaars bevat, was voorberei en gevalideer vir sy vermoë om glukose te oksideer. Deur middel van die gedrukte elektrodes met die vervaardigde ensiemoplossing te kombineer, was elektrochemiese tegnieke toegepas met die toevoeging van glukose om ’n elektrochemiese biosenser te realiseer. Glukose konsentrasies vanaf 0 32 mg=ml was getoets op die vervaardigde. Masters 2023-03-03T09:49:53Z 2023-05-18T07:03:23Z 2023-03-03T09:49:53Z 2023-05-18T07:03:23Z 2023-03 Thesis http://hdl.handle.net/10019.1/127082 en_ZA en_ZA Stellenbosch University, xii, 103 pages : illustrations application/pdf Stellenbosch : Stellenbosch University, |
| spellingShingle | Blood glucose monitoring Neonatology Biosensors Electrochemical sensors Francois, Snyders Towards a paper-based electrochemical glucose biosensor |
| title | Towards a paper-based electrochemical glucose biosensor |
| title_full | Towards a paper-based electrochemical glucose biosensor |
| title_fullStr | Towards a paper-based electrochemical glucose biosensor |
| title_full_unstemmed | Towards a paper-based electrochemical glucose biosensor |
| title_short | Towards a paper-based electrochemical glucose biosensor |
| title_sort | towards a paper based electrochemical glucose biosensor |
| topic | Blood glucose monitoring Neonatology Biosensors Electrochemical sensors |
| url | http://hdl.handle.net/10019.1/127082 |
| work_keys_str_mv | AT francoissnyders towardsapaperbasedelectrochemicalglucosebiosensor |