Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Gluten quantification methods and their compliance with the food safety legislation of South Africa.
Thesis (MScFoodSc)--Stellenbosch University, 2023.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
| Published: |
Stellenbosch : Stellenbosch University
2023
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613798188187648 |
|---|---|
| access_status_str | Open Access |
| author | Engelbrecht, Marzanne |
| author2 | Krugel, Maricel |
| author_browse | Engelbrecht, Marzanne Krugel, Maricel |
| author_facet | Krugel, Maricel Engelbrecht, Marzanne |
| author_sort | Engelbrecht, Marzanne |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MScFoodSc)--Stellenbosch University, 2023. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/127095 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:51.674Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/127095 Gluten quantification methods and their compliance with the food safety legislation of South Africa. Engelbrecht, Marzanne Krugel, Maricel Tait, Timo Dhanani, Karim Stellenbosch University. Faculty of Agrisciences. Dept. of Food Science. Gluten Celiac disease -- Nutritional aspects Food -- Gluten content Food -- Safety measures -- Standards -- South Africa Food law and legislation -- South Africa Food -- Labeling -- South Africa UCTD Thesis (MScFoodSc)--Stellenbosch University, 2023. ENGLISH ABSTRACT: Celiac disease is an adverse food reaction elicited by the seed-storage protein called gluten. Consequently, regulatory bodies have implemented legislative requirements for labelling gluten-free products and the testing thereof. However, during the last decade various researchers have indicated that food processing creates analytical challenges, such as reduced solubility and extractability of gluten protein from the food matrix, for reliable and accurate quantitation. These challenges, therefore, may cause a food safety risk for individuals suffering from celiac disease. The management of celiac disease is thus highly dependent on accurately quantifying gluten and labelling the product according to the food safety legislation of South Africa. This study describes: 1. The quantification of gluten in processed food samples as stipulated by the South African regulations relating to labelling and advertising of foodstuffs, R.146 of 1 March 2010. 2. The complexity of gluten proteins and analytical challenges for the accurate quantitation of gluten-free products. 3. The preparation of incurred food samples in a test kitchen facility and the brewing of barley-beer in a pilot-scale plant. 4. A comparison and examination of the efficacy and efficiency of the R5 enzyme-linked immunosorbent assay (ELISA) kits, the RIDASCREEN® Gliadin sandwich and RIDASCREEN® Gliadin competitive assays for the quantification of gluten from wheat, rye, and barley in food samples. 5. An examination into the fitness of the label-free targeted liquid chromatography- tandem mass spectrometry (LC-MS/MS) method for the quantification of gluten from wheat, rye, and barley in food samples. AFRIKAANSE OPSOMMING: Coeliakie is 'n nadelige voedselreaksie wat veroorsaak word deur die saadbergingsproteïen genaamd gluteen. Gevolglik het regulerende liggame wetgewende vereistes geïmplementeer vir die etikettering van gluteenvrye produkte en die toetsing daarvan. Gedurende die afgelope dekade het verskeie navorsers egter aangedui dat voedselprosessering analitiese uitdagings, soos verlaagde proteïenoplosbaarheid en die ekstraheerbaarheid van gluteenproteïen vanuit die voedselmatriks, skep vir betroubare en akkurate kwantifisering. Hierdie uitdagings kan dus 'n voedselveiligheidsrisiko veroorsaak vir individue wat aan coeliakiely. Die bestuur van coeliakie is gevolglik afhanklik van die akkurate kwantifisering van gluteen en etikettering van die voedselprodukte volgens die voedselveiligheidswetgewing van Suid-Afrika. Hierdie studie beskryf spesifiek: 1. Die kwantifisering van gluteen in geprosesserde voedselmonsters, soos bepaal deur die Suid-Afrikaanse regulasies, met betrekking tot etikettering en advertering van voedsel, R.146 van 1 Maart 2010. 2. Die kompleksiteit van gluteenproteïen en die analitiese uitdagings vir die akkurate kwantifisering en etikettering van gluteenvrye produkte. 3. Die voorbereiding van voedselmonsters in 'n kombuisfasiliteit en die brou van garsbier in 'n proefskaal aanleg. 4. ’n Vergelyking en ondersoek na die doeltreffendheid van die R5 ensiem-gekoppelde immuno-absorpsie essaïs (ELISA), naamlik die RIDASCREEN® Gliadien immunometriese en RIDASCREEN® Gliadien mededingende toetse vir die kwantifisering van gluteen afkomstig vanaf koring, rog en gars in voedselmonsters. 5. ’n Ondersoek na die geskiktheid van die etiketvrye geteikende vloeistofchromotografie massaspektrometrie-gekoppelde (LC-MS/MS) metode vir die kwantifisering van gluteen afkomstig vanaf koring, rog en gars in voedselmonsters. Masters 2023-02-21T18:13:47Z 2023-05-18T07:04:03Z 2023-02-21T18:13:47Z 2023-05-18T07:04:03Z 2023-03 Thesis http://hdl.handle.net/10019.1/127095 en_ZA Stellenbosch University xviii, 138 pages : illustrations (some color) application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Gluten Celiac disease -- Nutritional aspects Food -- Gluten content Food -- Safety measures -- Standards -- South Africa Food law and legislation -- South Africa Food -- Labeling -- South Africa UCTD Engelbrecht, Marzanne Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title | Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title_full | Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title_fullStr | Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title_full_unstemmed | Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title_short | Gluten quantification methods and their compliance with the food safety legislation of South Africa. |
| title_sort | gluten quantification methods and their compliance with the food safety legislation of south africa |
| topic | Gluten Celiac disease -- Nutritional aspects Food -- Gluten content Food -- Safety measures -- Standards -- South Africa Food law and legislation -- South Africa Food -- Labeling -- South Africa UCTD |
| url | http://hdl.handle.net/10019.1/127095 |
| work_keys_str_mv | AT engelbrechtmarzanne glutenquantificationmethodsandtheircompliancewiththefoodsafetylegislationofsouthafrica |