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Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy
Thesis (MEng)--Stellenbosch University, 2023.
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| Format: | Thesis |
| Language: | en_ZA en_ZA |
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Stellenbosch : Stellenbosch University
2023
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| _version_ | 1867614072345722880 |
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| access_status_str | Open Access |
| author | Hugo, Devlin |
| author2 | Perold, Willem |
| author_browse | Hugo, Devlin Perold, Willem |
| author_facet | Perold, Willem Hugo, Devlin |
| author_sort | Hugo, Devlin |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MEng)--Stellenbosch University, 2023. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/128909 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA en_ZA |
| last_indexed | 2026-06-10T12:46:13.197Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/128909 Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy Hugo, Devlin Perold, Willem Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering. Menopause -- Hormone therapy Estradiol biosensor Electrochemical sensors Transducers, Biomedical Thesis (MEng)--Stellenbosch University, 2023. ENGLISH ABSTRACT: 17-_ Estradiol de_ciency, as a consequence of menopause, is the cause of signi_cant discomfort and health risks for women. Current medical practice addresses this issue by utilising hormone replacement therapy (HRT) in order to treat the symptoms of 17-_estradiol de_ciency and it's subsequent health complications. Consequently, there exists a signi_cant need for an inexpensive point of care (POC) device that may be used to quantitatively detect 17-_ estradiol. The intended purpose of such a device is to monitor hormone levels to facilitate the accurate administration of HRT to patients in need. This research attempted to develop a quantitative electrochemical 17-_ estradiol biosensor which was comprised of three main design components. The _rst component was the development of a 17-_ estradiol functionalised transducer which was realised by immobilising 17-_ estradiol antibodies to a gold electrode surface. The immobilisation con_rmation, although it was resource intensive, was tested thoroughly and con_rmed by uorescence microscopy. The second component was the development of a potentiostat device that could perform square wave voltammetry (SWV) amongst a host of electroanalytical experiments on the electrochemical transducer. The developed device was simulated, built and veri_ed with acceptable results for the application. The third design component was the antigen testing procedure that was developed to extract usable quantitative 17-_ estradiol detection results. The developed biosensor prototype delivered promising results, with the sensor being capable of quantitative detection when using triplicates of the 17-_ estradiol transducer for sample testing. It was recommended for future research that the variability in the electrical characteristics of the transducers from manufacturing be reduced. Furthermore, it is believed that upon further optimisation of the antigen testing procedure that a commercialisable POC 17-_ estradiol biosensor may become a reality. AFRIKAANSE OPSOMMING: 17-_ Estradiol-tekort, as gevolg van menopouse, is die oorsaak van wesenlike ongemak en gesondheidsrisiko's by vroue. Huidige mediese praktyk spreek hierdie probleem aan deur hormoonvervangingsterapie (HVT) te gebruik om die simptome van 17-_ estradiol-tekort, en die gevolglike gesondheidskomplikasies, te behandel. Daar bestaan dus 'n beduidende behoefte aan 'n goedkoop punt van sorg (PVS) toestel wat gebruik kan word om 17-_ estradiol kwantitatief op te spoor. Die beoogde doel van so 'n toestel is om hormoonvlakke te monitor om die akkurate toediening van HVT aan pasiente in nood te fasiliteer. Die navorsing het gepoog om 'n kwantitatiewe elektrochemiese 17-_ estradiol biosensor te ontwikkel wat uit drie hoofontwerpkomponente bestaan. Die eerste komponent was 'n 17-_ estradiol-gefunksionaliseerde omsetter wat gerealiseer is deur 17-_ estradiol teenliggaampies op 'n goue elektrode-oppervlak te immobiliseer . Die immobilisasiebevestiging, alhoewel hulpbronintensief, is deeglik getoets en deur uoressensiemikroskopie bevestig. Die tweede komponent was die ontwikkeling van 'n potensiostaat toestel wat SWV kon uitvoer op 'n magdom elektro-analitiese eksperimente op die elektrochemiese omsetter. Die ontwikkelde toestel is gesimuleer, gebou en geveri_eer met baie bevredigende resultate. Die derde ontwerpkomponent was die antigeentoetsprosedure wat gevolg en verbeter is om bruikbare kwantitatiewe 17-_ estradiol opsporingsresultate te onttrek. Die ontwikkelde biosensor-prototipe het groot belofte getoon, met die resultate wat in staat was tot kwantitatiewe opsporing wanneer triplikate van die 17-_ estradiol-omskakelaar vir die toets gebruik is. Dit is vir toekomstige navorsing aanbeveel dat die variasie in die elektriese eienskappe van die omskakelaars vanaf vervaardiging verminder behoort te word. Verder word geglo dat met verdere optimalisering van die antigeentoetsprosedure, 'n kommersialiseerbare PVT 17-_ estradiol biosensor 'n werklikheid kan word. Masters 2023-11-20T09:04:33Z 2024-01-08T15:27:11Z 2023-11-20T09:04:33Z 2024-01-08T15:27:11Z 2023-12 Thesis https://scholar.sun.ac.za/handle/10019.1/128909 en_ZA en_ZA Stellenbosch University xv, 147 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Menopause -- Hormone therapy Estradiol biosensor Electrochemical sensors Transducers, Biomedical Hugo, Devlin Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title | Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title_full | Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title_fullStr | Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title_full_unstemmed | Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title_short | Development of a 17-β estradiol biosensor towards the improvement of hormone replacement therapy |
| title_sort | development of a 17 β estradiol biosensor towards the improvement of hormone replacement therapy |
| topic | Menopause -- Hormone therapy Estradiol biosensor Electrochemical sensors Transducers, Biomedical |
| url | https://scholar.sun.ac.za/handle/10019.1/128909 |
| work_keys_str_mv | AT hugodevlin developmentofa17bestradiolbiosensortowardstheimprovementofhormonereplacementtherapy |