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Development of a layered electrochemical biosensor
Thesis (PhD)--Stellenbosch University, 2024.
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| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA en_ZA |
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Stellenbosch : Stellenbosch University
2024
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| _version_ | 1867614032716890112 |
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| access_status_str | Open Access |
| author | Schoeman, Stephan |
| author2 | Perold, Willem |
| author_browse | Perold, Willem Schoeman, Stephan |
| author_facet | Perold, Willem Schoeman, Stephan |
| author_sort | Schoeman, Stephan |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (PhD)--Stellenbosch University, 2024. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/130295 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA en_ZA |
| last_indexed | 2026-06-10T12:45:35.384Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/130295 Development of a layered electrochemical biosensor Schoeman, Stephan Perold, Willem Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering. Development of a layered electrochemical biosensor Electrochemical sensors Biosensors Tuberculosis -- Pathogenesis Prototypes, Engineering UCTD Thesis (PhD)--Stellenbosch University, 2024. ENGLISH OPSOMMING: Tuberculosis continues to be a major global health challenge, with 10.6 million cases reported in 2021. South Africa faces a considerable tuberculosis problem, one of which is a high rate of undiagnosed cases. The prevalent tuberculosis detection methods in South Africa are plagued by inefficiencies, which contribute to the under-detection of the disease amongst those infected, in turn highlighting the need for more effective screening tools. In response to this, the World Health Organisation has prioritised the development of new diagnostic methods. This thesis contributes to these efforts by developing a biosensor for TB diagnosis, demonstrating the functionality of the developed device by quantifying a specific protein associated with the disease, thereby aligning with the targets for tuberculosis prevention, care, and control. The study includes an analysis of a commercial electrochemical sensor to establish a baseline for sensor attributes and functionalities. A series of prototypes were developed, each contributing to the iterative refinement of the sensor design. The prototypes explored various aspects of the device, progressively addressing manufacturing challenges noted throughout the development of the different prototypes. The project reached its zenith with Sensor M, which successfully combined the innovations developed throughout the process to quantify C-reactive protein. The device demonstrated a detection range from 10 ng/ml to 1 pg/ml and featured a control electrode for experimental validation. Within the electrochemical biosensing field the project made substantial contributions towards TB biomarker detection, and paves the way for future development in electrochemical lateral flow biosensor technology. AFRIKAANSE OPSOMMING: Tuberkulose is steeds ’n wˆereldwye gesondheidskwessie, met 10.6 miljoen gevalle wat in 2021 aangemeld is. Suid-Afrika staar ’n tuberkuloseprobleem in die gesig, waarvan een ’n ho¨e koers van ongediagnoseerde gevalle is. Die mees algemene tuberkulose-opsporingsmetodes wat in Suid-Afrika voorkom word gekenmerk deur ondoeltreffendheid, wat die behoefte aan meer effektiewe opsporingsmetode onderstreep. Die Wˆereldgesondheidsorganisasie het klem gelˆe op die ontwikkeling van nuwe diagnostiese metodes om die siekte op te spoor en dra hierdie tesis by tot die bogenoemde pogings, deur ’n biosensor vir TB-diagnose te ontwikkel. Die funksionaliteit van die ontwikkelde toestel word demonstreer deur die kwantifisering van ’n spesifieke prote¨ıen wat met die siekte geassosieer word. Die studie sluit ’n ontleding van ’n kommersi¨ele elektrochemiese sensor in om ’n basislyn vir sensorkenmerke en -funksionaliteite vas te stel. ’n Reeks prototipes is ontwikkel, wat elk bygedra het tot die iteratiewe verfyning van die sensorontwerp. Die prototipes het verskeie aspekte van die toestel ondersoek en vervaardigingsuitdagings wat regdeur die ontwikkeling van die verskillende prototipes opgemerk is, geleidelik aangespreek. Die projek het sy hoogtepunt bereik met Sensor M, wat die innovasies wat deur die proses ontwikkel is, suksesvol gekombineer het om C-reaktiewe prote¨ıen te kwantifiseer. Die toestel het ’n opsporingskonsentrasie reeks van 10 ng/ml tot 1 pg/ml getoon en het ’n kontrole-elektrode vir eksperimentele stawing. Binne die elektrochemiese biosensor-veld het die projek aansienlike bydraes gelewer tot TB- biomerkeropsporing, waar die ontwikkeling van die toeste die weg baan vir toekomstige ontwikkeling in elektrochemiese laterale vloei-biosensortegnologie. Doctorate 2024-03-04T07:11:33Z 2024-04-26T12:24:34Z 2024-03-04T07:11:33Z 2024-04-26T12:24:34Z 2024-03 Thesis https://scholar.sun.ac.za/handle/10019.1/130295 en_ZA en_ZA Stellenbosch University xix, 251 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Development of a layered electrochemical biosensor Electrochemical sensors Biosensors Tuberculosis -- Pathogenesis Prototypes, Engineering UCTD Schoeman, Stephan Development of a layered electrochemical biosensor |
| title | Development of a layered electrochemical biosensor |
| title_full | Development of a layered electrochemical biosensor |
| title_fullStr | Development of a layered electrochemical biosensor |
| title_full_unstemmed | Development of a layered electrochemical biosensor |
| title_short | Development of a layered electrochemical biosensor |
| title_sort | development of a layered electrochemical biosensor |
| topic | Development of a layered electrochemical biosensor Electrochemical sensors Biosensors Tuberculosis -- Pathogenesis Prototypes, Engineering UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/130295 |
| work_keys_str_mv | AT schoemanstephan developmentofalayeredelectrochemicalbiosensor |