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A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART)
Thesis (MSc)--Stellenbosch University, 2024.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867614068312899584 |
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| access_status_str | Open Access |
| author | Broom, Jessica Nicole |
| author2 | Erasmus, Evelyn |
| author_browse | Broom, Jessica Nicole Erasmus, Evelyn |
| author_facet | Erasmus, Evelyn Broom, Jessica Nicole |
| author_sort | Broom, Jessica Nicole |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description |
Thesis (MSc)--Stellenbosch University, 2024. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/131604 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:46:09.042Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/131604 A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) Broom, Jessica Nicole Erasmus, Evelyn Burger, Riana Oosthuizen, Micke Matsaseng, Thabo Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences. Reproductive technology Infertility -- Treatment Polycystic ovary syndrome Ovum -- Research UCTD Thesis (MSc)--Stellenbosch University, 2024. ENGLISH ABSTRACT: Background: In the past, fertility treatments relied on the female’s natural menstrual cycle, thereby resulting in the retrieval of a single oocyte per cycle. This often necessitated multiple treatment cycles for a successful outcome. However, with the development of Controlled Ovarian Hyperstimulation (COH), the focus shifted to the recruitment and stimulation of multiple follicles in an attempt to obtain a larger number of oocytes during a single aspiration. This technique, devised under the premise that a higher oocyte yield improves Assisted Reproductive Technology (ART) success, in terms of clinical pregnancy and live birth rates, is commonly implemented in ART cycles globally today. While this technique is typically well-established, patient outcomes – specifically with regards to the number of follicles and subsequent oocytes retrieved – vary significantly. The optimal number of oocytes required for successful laboratory and clinical outcomes thus remains a contentious topic within scientific communities. Although several studies have investigated this topic in the context of conventional stimulation, conflicting results are reported in the literature. Furthermore, few studies have investigated this topic within the framework of mild stimulation protocols. This study was therefore undertaken to further examine this topic under the laboratory conditions of Tygerberg Fertility Clinic, aiming to expand upon the limited research available in the literature. Aims and Objectives: The primary aim was to retrospectively evaluate the impact of oocyte number on the clinical pregnancy rate and live birth rate. The secondary aim was to retrospectively evaluate the impact of oocyte number on the miscarriage rate, implantation rate, fertilization rate, and blastulation rate. Materials and Methods: This study was conducted in a retrospective manner with data acquired from the routine laboratory records of Tygerberg Fertility Clinic for the period January 2019 – June 2023. The study population was divided into three distinct categories according to the MII oocyte number observed during oocyte aspiration. These groups were defined as follows: those with MII oocyte numbers ranging from 1 to 3 [110 patients] (Group A), those with 4 to 6 MII oocytes [72 patients] (Group B), and those with 7 or more MII oocytes retrieved [55 patients] (Group C). The data was also categorized according to the pre-determined inclusion and exclusion criteria and was adjusted for additional co-founding variables. Statistical analysis was conducted on the data, with a p-value of < 0.05 indicating statistical significance. Results: The FR (A: 86.21%; B: 85.63%; C: 85.93%), IR (A: 14.09%; B: 21.76%; C: 25.45%) and MR (A: 16.67%; B: 19.05%; C:15.79%) were not statistically significant across the oocyte groups A, B and C (p>0.05). The BR (A: 73.11%; B: 62.10%; C: 53.42%), CPR (A: 16.36%; B: 30.56%; C: 34.55%) and the LBR (A: 13.64%; B: 25.00%; C: 29.09%) were statistically significant across the various groups A, B and C (p<0.05), with improved ART outcomes in group B and C. Conclusion: The current study compared three groups of varying oocyte yields (A, B and C) in order to determine an optimal oocyte yield in a mild ovarian stimulation setting. Significant differences were observed with regards to the BR, CPR and LBR between the three groups. Conversely, comparable IRs, MRs and FRs were noted. Similar to findings reported in several studies, the results of the present study demonstrated a positive correlation between increased oocyte yields and favourable ART outcomes. Based on the findings of this retrospective study, an optimal oocyte number greater than 7 is recommended with the use of mild stimulation protocols; however, further research is required to validate the impact of this oocyte number. This study hence provides valuable insight into the relationship between oocyte yields and mild stimulation protocols. AFRIKAANSE OPSOMMING: Agtergrond: In die verlede het fertiliteitbehandeling op die natuurlike menstruele siklus van die vrou staatgemaak, wat daartoe gelei het dat slegs een oösiet per siklus verkry was. Dit het dikwels vereis dat veelvuldige behandelingsiklusse noodsaaklik was vir 'n suksesvolle uitkoms. Met die ontwikkeling van Gekontroleerde Ovariële Stimulasie het die fokus egter na die stimulasie van veelvuldige follikels verskuif, om ’n groter hoeveelheid oösiete te verkry gedurende ’n enkele aspirasie. Hierdie tegniek word nou gereeld gebruik om ’n hoër oösietopbrengs te lewer, en dra by tot suksesvolle ART siklusse. Alhoewel hierdie gevestigdetegniek nou algemeen in gebruik is, verskil pasiëntuitkomste aansienlik, veral met betrekking tot die aantal follikels en die verkryging van oösiete. Die bepaling van die optimalehoeveelheid oösiete vir suksesvolle kliniese en laboratorium uitkomste bly ‘n kontroversiële onderwerp. Alhoewel verskeie studies reeds die onderwerp van konvensionele stimulasie ondersoek het, is teenstrydige resultate gepubliseer. ‘n Beperkte aantal studies het ligte stimulasieprotokolle ondersoek. Die huidige studie is dus onderneem om die kwessie verder te ondersoek en om die beperkte navorsing aan te vul. Doelwitte: Die primêre doelwit was om die impak van oösietgetal op die kliniese swangerskapkoers en lewende geboortekoers retrospektief te evalueer. Die sekondêre doelwit was om die impak van oösietgetal op die miskraamkoers, implantasie, bevrugtingskoers en blastulasiekoers retrospektief te evalueer. Materiale en Metodes: Hierdie studie is retrospektief uitgevoer op data wat verkry was uit laboratoriumrekords van die Tygerberg Fertiliteitskliniek, vir die periode Januarie 2019 tot Junie 2023. Die studiepopulasie is in drie onderskeie groepe ingedeel volgens die aantal MIIoösiete wat tydens aspirasie verkry was. Hierdie groepe is as volg geklasifiseer: dié met MIIoösietgetalle van 1 tot 3 [110 pasiënte] (Groep A), dié met 4 tot 6 MII-oösiete [72 pasiënte] (Groep B), en dié met 7 of meer MII-oösiete [55 pasiënte] (Groep C). Die data is ook gekategoriseer volgens die voorafbepaalde insluitings- en uitsluitingskriteria. Statistiese analise is op die data uitgevoer, met 'n p-waarde van < 0.05 wat statistiese betekenisvolheid aandui. Resultate: Die FR (A: 86.21%; B: 85.63%; C: 85.93%), IR (A: 14.09%; B: 21.76%; C: 25.45%) en MR (A: 16.67%; B: 19.05%; C:15.79%) was nie statisties betekenisvol tussen die oösiet groepe A, B en C (p>0.05) nie. Die BR (A: 73.11%; B: 62.10%; C: 53.42%), CPR (A: 16.36%; B: 30.56%; C: 34.55%) en die LBR (A: 13.64%; B: 25.00%; C: 29.09%) was statisties betekenisvol verskillend tussen die groepe A,B en C (p<0.05), met beter uitkomste in groepe B en C. Gevolgtrekking: Hierdie studie het drie groepe van verskillende oösietopbrengste (A, B en C) vergelyk om 'n optimale oösietopbrengs in 'n ligte stimulasie omgewing te bepaal. Beduidende verskille is waargeneem met betrekking tot die blastulasiekoers, kliniese swangerskapkoers en lewende geboortekoers tussen die drie groepe. Die implantasie, miskraamkoerse en bevrugtingskoerse was egter vergelykbaar. In ooreenstemming met vorige studies is 'n positiewe korrelasie tussen verhoogde oösietopbrengste en gunstige ARTuitkomste gevind. Op grond van die bevindinge van hierdie studie, word 'n optimale oösietgetal van meer as 7 aanbeveel tydens ligte stimulasie protokolle. Verdere navorsing is egter nodig om die impak van hierdie oösietgetal te bevestig. Hierdie studie verskaf dus waardevolle inligting met betreking tot die verhouding tussen oösietopbrengste en ligte stimulasie protokolle. Masters 2025-01-28T14:10:04Z 2025-01-28T14:10:04Z 2024-12 Thesis https://scholar.sun.ac.za/handle/10019.1/131604 en Stellenbosch University 128 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Reproductive technology Infertility -- Treatment Polycystic ovary syndrome Ovum -- Research UCTD Broom, Jessica Nicole A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title | A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title_full | A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title_fullStr | A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title_full_unstemmed | A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title_short | A retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology (ART) |
| title_sort | retrospective study of oocyte yield on reproductive outcomes in assisted reproductive technology art |
| topic | Reproductive technology Infertility -- Treatment Polycystic ovary syndrome Ovum -- Research UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/131604 |
| work_keys_str_mv | AT broomjessicanicole aretrospectivestudyofoocyteyieldonreproductiveoutcomesinassistedreproductivetechnologyart AT broomjessicanicole retrospectivestudyofoocyteyieldonreproductiveoutcomesinassistedreproductivetechnologyart |