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Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model
Thesis (MSc)--Stellenbosch University, 2024.
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867613917859020800 |
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| access_status_str | Open Access |
| author | Le Roux, Kyle |
| author2 | Abdul, Naeem Sheik |
| author_browse | Abdul, Naeem Sheik Le Roux, Kyle |
| author_facet | Abdul, Naeem Sheik Le Roux, Kyle |
| author_sort | Le Roux, Kyle |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2024. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/131795 |
| institution | Stellenbosch University (South Africa) |
| last_indexed | 2026-06-10T12:43:46.104Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/131795 Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model Le Roux, Kyle Abdul, Naeem Sheik Verhoog, Nicolette J. D. Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Type 2 diabetes -- South Africa Type 2 diabetes -- Treatment Mitochondrial DNA Honeybush (Cyclopia spp.) -- Therapeutic use Herbs -- Therapeutic use Antioxidants -- Health aspects Oxidative stress Phenols -- Physiological effect UCTD Thesis (MSc)--Stellenbosch University, 2024. ENGLISH ABSTRACT: Type 2 diabetes (T2DM) has become a major concern in South Africa due to a surge in disease prevalence over the past decade, making it one of the countries with the highest diabetes prevalence in Africa. Hyperglycaemia is a hallmark of T2DM and the resultant glucotoxic environment damages hepatic cells through mechanisms involving oxidative stress and mitochondrial dysfunction. Conventional treatment programmes have been ineffective in stopping the acceleration of the diabetic crisis in South Africa due a combination of socioeconomic factors, highlighting the need for a novel approach which can accommodate South African diabetics and their needs. Research has highlighted the potential of incorporating ethnopharmacology into treatment regimens to improve affordability and accessibility, this is supported by growing evidence for the potential of plantderived phenolic compounds (PPCs) and their effects against chronic disease pathophysiology. Ethnopharmacology could present as a novel approach to T2DM treatment in South Africa, as the use of indigenous herbal medicines has cultural and historical significance for many in the country. Honeybush (Cyclopia spp.) is an indigenous tea plant which over recent years has garnered more public attention, both commercially for its unique flavour profile and academically for its antioxidant-rich chemical profile. Over the last 15 years, studies have spotlighted Honeybush for its bioactive compound profile and potential as an intervention against chronic disease. While research has shown the applicability of Honeybush treatment to diseases associated with oxidative stress, this study is the first to explore the effects of Honeybush treatment on glucotoxicity in a liver cell model. This present study set out to ascertain the applicability of Honeybush as a treatment against T2DM-induced mitochondrial dysfunction and oxidative stress in a high-glucose in vitro model. A methanolic C. subternata extract previously studied for phytoestrogenic properties, SM6Met, was evaluated for its effects on oxidative stress and mitochondrial dysfunction as well as the protein expression of mitochondrial and antioxidant proteins in HepG2 liver cells under glucotoxic stress. SM6Met was shown to significantly improve metabolic activity, cellular ATP levels and total reduced glutathione (GSH) concentrations whilst reducing cytotoxicity. SM6Met was shown to increase the expression of cytoplasmic antioxidant enzymes (Trx1 and catalase), mitochondrial protective enzymes (SOD2, LonP1 and Sirt3). These effects were dependent on the increased expression of Nrf2 and PGC- these transcription factors are regarded as the master regulators of the cell antioxidant response. Taken together the data suggests that SM6Met enhances the cellular antioxidant defence capacity and may provide a relevant supportive therapeutic option for treating diabetic complications associated with oxidative stress and mitochondrial dysfunction. AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar. Masters 2025-03-25T10:22:11Z 2025-03-25T10:22:11Z 2024-12 Thesis https://scholar.sun.ac.za/handle/10019.1/131795 Stellenbosch University xii, 93 pages : illustrations, maps application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Type 2 diabetes -- South Africa Type 2 diabetes -- Treatment Mitochondrial DNA Honeybush (Cyclopia spp.) -- Therapeutic use Herbs -- Therapeutic use Antioxidants -- Health aspects Oxidative stress Phenols -- Physiological effect UCTD Le Roux, Kyle Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title | Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title_full | Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title_fullStr | Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title_full_unstemmed | Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title_short | Honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| title_sort | honeybush extract as a viable intervention against oxidative stress and mitochondrial dysfunction in a glucotoxic in vitro model |
| topic | Type 2 diabetes -- South Africa Type 2 diabetes -- Treatment Mitochondrial DNA Honeybush (Cyclopia spp.) -- Therapeutic use Herbs -- Therapeutic use Antioxidants -- Health aspects Oxidative stress Phenols -- Physiological effect UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/131795 |
| work_keys_str_mv | AT lerouxkyle honeybushextractasaviableinterventionagainstoxidativestressandmitochondrialdysfunctioninaglucotoxicinvitromodel |