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Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function
Thesis (MSc)--Stellenbosch University, 2024.
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867613885481091072 |
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| access_status_str | Open Access |
| author | Shaboodien, Sara |
| author2 | Maarman, Gerald |
| author_browse | Maarman, Gerald Shaboodien, Sara |
| author_facet | Maarman, Gerald Shaboodien, Sara |
| author_sort | Shaboodien, Sara |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description |
Thesis (MSc)--Stellenbosch University, 2024. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/131904 |
| institution | Stellenbosch University (South Africa) |
| last_indexed | 2026-06-10T12:43:14.822Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/131904 Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function Shaboodien, Sara Maarman, Gerald Johnson, Rabia Marais, Erna Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Medical Physiology. Rooibos tea -- Therapeutic use Cardiovascular system -- Diseases Isoproterenol Mitochondria UCTD Thesis (MSc)--Stellenbosch University, 2024. ENGLISH ABSTRACT: Background: Cardiovascular disease (CVD) is a global health threat associated with risk factors such as systemic hypertension. The increased blood pressure places severe stress on the left ventricle, and if this persists, it results in left ventricular hypertrophy and subsequently, left ventricular failure. Most pharmacological treatments have long-term side effects and are costly, especially for patients who reside in low-resource settings. Therefore, there is a growing need to identify more natural and affordable adjuvant therapies, to assist in managing CVD. This project aimed to determine whether Aspalathus linearis (Rooibos, RB) protects against isoproterenol-induced hypertrophy of H9c2 cardiomyoblasts. Methods: Cardiomyoblasts were treated with either isoproterenol (50 μM, 24 hours), RB (100 μg/mL, 24 hours), co-treatment with RB (100 μg/mL, 24 hours) and isoproterenol (50 μM, 24 hours), or control (2% fetal bovine serum (FBS), 24 hours). The following experiments were performed: cell viability assay (MTT), cell size (light microscopy), antioxidant assays (superoxide dismutase (SOD) and catalase (CAT)), lipid peroxidation assay (thiobarbituric acid reactive substances (TBARS)), Western blot analysis, high-resolution respirometry (Oroboros) and an adenosine triphosphate (ATP) assay. A one-way ANOVA analysis followed by a Bonferroni multiple comparison as a post hoc test or an unpaired Student’s t-test where appropriate was performed for all data. Results: Isoproterenol treatment increased cell size, reduced viability, reduced the activities of SOD and CAT, increased lipid peroxidation, reduced mitochondrial routine respiration, reduced complex-1 linked oxidative phosphorylation (OXPHOS), reduced complex-II linked OXPHOS via the S-pathway, and elevated complex-4 activity without negatively impacting ATP levels. Co-treatment with RB and isoproterenol reduced cell size, improved antioxidant enzyme activity, increased routine respiration complex-I linked OXPHOS, and reduced the cytochrome-c response, while increasing the expression of total Akt and reducing nuclear factor of activated T-cells (NFAT) expression. Conclusion: The data suggests that RB may be a potential adjuvant therapy in treating left ventricular hypertrophy. The protective effects of RB in this context are ascribed to its potent antioxidant action and rescuing effect on mitochondrial dysfunction in left ventricular hypertrophy. AFRIKAANSE OPSOMMING: Agtergrond: Kardiovaskulêre siekte (KVS) is 'n wêreldwye gesondheidsbedreiging wat verband hou met risikofaktore soos sistemiese hipertensie. Die verhoogde bloeddruk plaas erge spanning op die linkerventrikel, en as dit voortduur, lei dit tot linkerventrikulêre hipertrofie en gevolglik linkerventrikulêre mislukking. Die meeste farmakologiese behandelings het langtermyn newe-effekte en is duur, veral vir pasiënte wat in lae-hulpbron omgewings woon. Daarom is daar 'n strewe om bykomende terapieë te identifiseer wat meer natuurlik en bekostigbaar is, om te help met die bestuur van CVD. Hierdie projek het ten doel gehad om te bepaal of Aspalathus linearis (Rooibos, RB) beskerm teen isoproterenol-geïnduseerde hipertrofie van H9c2 kardiomioblaste. Metodes: Kardiomioblaste is blootgestel aan óf isoproterenol (50 µM, 24 uur), RB (100 µg/ml, 24 uur), gesamentlike behandeling met RB (100 µg/ml, 24 uur) en isoproterenol (50 µM, 24 uur), of beheerselle (2% fetale bees serum (FBS), 24 uur). Ons het die volgende toetse uitgevoer: lewensvatbaarheid (MTT), selgrootte (ligmikroskopie), antioksidant toets (superoksieddismutase (SOD) en katalase (CAT)), lipiedperoksidasie (tiobarbituursuurreaktiewe stowwe (TBARS)), Western blots, hoë-resolusie respirometrie (Oroboros) en 'n adenosientrifosfaat (ATP) toets. 'n Eenrigting ANOVA-analise gevolg deur 'n Bonferronimeervoudige vergelyking as 'n post hoc-toets of 'n ongepaarde Student se t-toets waar toepaslik is vir alle data uitgevoer. Resultate: Isoproterenol verhoog selgrootte, verminder lewensvatbaarheid, verminder die aktiwiteite van SOD en CAT, verhoogde oksidatiewe stres, verminder mitochondriale roetinerespirasie, verminder kompleks-I gekoppelde oksidatiewe fosforilering (OXPHOS), verminder kompleks-II gekoppelde OXPHOS via die S-weg, en verhoogde kompleks-IV aktiwiteit sonder om ATP-vlakke negatief te beïnvloed. Gesamentlike behandeling met isoproterenol en RB het selgrootte verminder, antioksidant-ensiemaktiwiteit verbeter, roetine-respirasie kompleks-I gekoppelde OXPHOS verhoog, en die sitochroom-c reaksie verminder, terwyl die uitdrukking van totaal Akt verhoog word en NFAT uitdrukking verminder. Masters 2025-04-08T09:37:19Z 2025-04-08T09:37:19Z 2024-12 Thesis https://scholar.sun.ac.za/handle/10019.1/131904 Stellenbosch University 154 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Rooibos tea -- Therapeutic use Cardiovascular system -- Diseases Isoproterenol Mitochondria UCTD Shaboodien, Sara Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title | Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title_full | Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title_fullStr | Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title_full_unstemmed | Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title_short | Rooibos (Aspalathus linearis) exerts a protective effect against isoproterenol-induced hypertrophy in H9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| title_sort | rooibos aspalathus linearis exerts a protective effect against isoproterenol induced hypertrophy in h9c2 cardiomyoblasts by enhancing antioxidative ability and mitochondrial function |
| topic | Rooibos tea -- Therapeutic use Cardiovascular system -- Diseases Isoproterenol Mitochondria UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/131904 |
| work_keys_str_mv | AT shaboodiensara rooibosaspalathuslinearisexertsaprotectiveeffectagainstisoproterenolinducedhypertrophyinh9c2cardiomyoblastsbyenhancingantioxidativeabilityandmitochondrialfunction |