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The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids
Thesis (MSc) -- Stellenbosch University, 1995.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2011
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| _version_ | 1867613805232521216 |
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| access_status_str | Open Access |
| author | Grobler, Jandre |
| author2 | Van Vuuren, H. J. J. |
| author_browse | Grobler, Jandre Van Vuuren, H. J. J. |
| author_facet | Van Vuuren, H. J. J. Grobler, Jandre |
| author_sort | Grobler, Jandre |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc) -- Stellenbosch University, 1995. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/13199 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:41:58.332Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/13199 The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids Grobler, Jandre Van Vuuren, H. J. J. Stellenbosch University. Faculty of Science. Dept. of Microbiology. Schizosaccharomyces pombe -- Genetics Fermentation Stellenbosch University Dissertations Microbiology Thesis (MSc) -- Stellenbosch University, 1995. During the primary fermentation of wine, grape must is fermented to ethanol, carbon dioxide and flavour compounds by strains of Saccharomyces cerevisiae. In the secondary malolactic fermentation, L-malic acid is decarboxylated to L(+)-lactic acid by lactic acid bacteria, in particular selected strains of Leuconostoc oenos. This deacidification of wine is of great technological interest to wineries. The malolactic fermentation, however, is a difficult fermentation to manage as malolactic bacteria are sensitive to high ethanol and sulphur dioxide concentrations. Moreover, at the end of the alcoholic fermentation limited nutrients are available for growth of the malolactic bacteria. Strains of S. cerevisiae cannot degrade malate effectively at wine pH. By contrast, strains of the fission yeast Schizosaccharomyces pombe can effectively degrade malate in the presence of an assimilable carbon source. Kinetic studies have revealed that L-malate is transported by an active protein symport system in S. pombe. Succinic, D-malic, fumaric, oxaloacetic, α-ketoglutaric, maleic and malonic acids all act as competitive inhibitors of L-malate transport in S. pombe. The malate permease gene (mae1) of S. pombe was cloned by complementation of a transport mutant using a S. pombe Sau3A genomic library. Sequencing revealed two open reading frames (ORFs). The first open reading frame codes for a protein (mae1p) with a predicted molecular weight of approximately 49 kDa. The hydropathy profile of the predicted amino acid sequence revealed a protein with hydrophilic N and C termini and 10 membrane-spanning or associated domains. These features are typical of transport proteins. Several conserved motifs were recognized in the primary sequence of mae1p by computer analysis. A well conserved PEST region and leucine-zipper consisting of four leucine residues spaced by six amino acids were identified. Furthermore, the protein contains several N-glycosylation sites. Complementation of the malate permease mutant with the mae1 fragment fully restored transport of malate and succinate. A prominent TATAT sequence (repeated four times) was located at ~66 to ~87 bp upstream of the ATG codon. A direct 10-bp repeat, TCATTTTTA, separated by 9 bp was found at positions -194 to -203 and -213 to -222. Chromoblotting revealed that the mae1 gene was located on chromosome 1. Northern analysis indicated that the mae1 gene was expressed constitutively and was not subject to catabolite repression, as was previously reported for the malate system genes of Candida utilis and Hansenula anomala. Masters 2011-05-15T16:05:35Z 2011-05-15T16:05:35Z 1995-12 Thesis http://hdl.handle.net/10019.1/13199 en Stellenbosch University 89 pages application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Schizosaccharomyces pombe -- Genetics Fermentation Stellenbosch University Dissertations Microbiology Grobler, Jandre The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title | The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title_full | The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title_fullStr | The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title_full_unstemmed | The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title_short | The mae1 gene of Schizosaccharomyces pombe encodes a permease for malate and other C4 dicarboxylic acids |
| title_sort | mae1 gene of schizosaccharomyces pombe encodes a permease for malate and other c4 dicarboxylic acids |
| topic | Schizosaccharomyces pombe -- Genetics Fermentation Stellenbosch University Dissertations Microbiology |
| url | http://hdl.handle.net/10019.1/13199 |
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