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Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response
Thesis (MSc)--Stellenbosch University, 2025.
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867613923606265856 |
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| access_status_str | Open Access |
| author | Du Plessis, Le Roi Alexander |
| author2 | Neethling, Pieter H.
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| author_browse | Du Plessis, Le Roi Alexander Neethling, Pieter H. |
| author_facet | Neethling, Pieter H.
Du Plessis, Le Roi Alexander |
| author_sort | Du Plessis, Le Roi Alexander |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/132148 |
| institution | Stellenbosch University (South Africa) |
| last_indexed | 2026-06-10T12:43:51.865Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/132148 Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response Du Plessis, Le Roi Alexander Neethling, Pieter H. Bosman, Gurthwin W. Stellenbosch University. Faculty of Science. Dept. of Physics. Optical tweezers Microfluidic devices -- Mathematical models Biological systems -- Measurement Cells -- Morphology Saccharomyces cerevisiae -- Effect of stress on Fluorescence microscopy -- Technique NAD (Coenzyme) -- Mechanism of action UCTD Thesis (MSc)--Stellenbosch University, 2025. Du Plessis, J. 2025. Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response. Unpublished doctoral dissertation. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/76d398bf-f80c-497e-94d2-6481cd1be10c ENGLISH ABSTRACT: Optical Tweezers are a tool which uses the radiation pressure from light to exert forces onto single particles to ultimately trap and isolate said particles. Initial use of optical tweezers were for quantitively measuring the forces in biological systems. With the advent of affordable microfluidic devices, new systems which incorporate optical tweezers for single cell manipulation and sorting have emerged. One potential that such an integrated system offers is the ability to conduct single cell studies where the optical tweezers are used to trap a single cell, while the microfluidic device controls the extracellular environment. This thesis presents such a system which measures the responses of a single cell’s morphology and metabolism to changes in the extracellular environment. The system used brightfield microscopy to monitor morphological changes and a fluorescence module to monitor changes in metabolism using endogenous fluorescence from NAD(P)H and FAD. The system was characterised in terms of various parameters and important aspects needed for stable, long-term measurements are discussed. Saccharomyces cerevisiae, also known as baker’s yeast, are easy to trap and are an excellent option for initial single cell experiments. The response of single yeast cells to osmotic shock and ethanol-induced oxidative stress, two well known and understood phenomena, was used to validate the integrated system. The results revealed the ability of the integrated system to monitor real-time changes in cell behaviour and showcases the ability that the system has as a drug screening platform. AFRIKAANSE OPSOMMING: ’n Optiese pincet gebruik die stralingsdruk van lig om kragte op enkele deeltjies uit te oefen, en hulle te vang en te isoleer. Die aanvanklike gebruik was vir die kwantitatiewe meting van die kragte in biologiese stelsels. Met die onlangse be- skikbaarheid van bekostigbare mikrofluïdiese toestelle is nuwe stelsels ontwikkel wat ’n optiese pincet gebruik vir enkelselmanipulasie en sortering. Een opsie wat so ’n geïntegreerde stelsel bied, is die vermoë om ’n enkele sel vas te vang, terwyl die mi- krofluïdiese toestel die ekstrasellulêre omgewing beheer. Hierdie proefskrif bied so ’n stelsel aan wat die invloed van veranderinge in die ekstrasellulêre omgewing van ’n enkele sel op sy morfologie en metabolisme meet. Die stelsel het helderveldmikro- skopie gebruik om morfologiese veranderinge te monitor en ’n fluoressensiemodule om veranderinge in metabolisme te monitor met behulp van endogene fluoressen- sie van NAD(P)H en FAD. Die stelsel is gekarakteriseer in terme van parameters belangrik vir stabiele, langtermynmetings. Saccharomyces cerevisiae, (bakkersgis), is maklik om vas te vang en is ’n uitstekende opsie vir aanvanklike enkelselekspe- rimente. Die reaksie van enkele gisselle op osmotiese skok en etanol-geïnduseerde oksidatiewe stres is gebruik om die geïntegreerde stelsel te evalueer. Die resultate het die vermoë van die geïntegreerde stelsel om intydse veranderinge in selgedrag te monitor getoon en gewys dat die sisteem as dwelmsiftingsplatform kan dien. Masters 2025-05-27T14:12:03Z 2025-05-27T14:12:03Z 2025-03 Thesis https://scholar.sun.ac.za/handle/10019.1/132148 Stellenbosch University xii, 77 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Optical tweezers Microfluidic devices -- Mathematical models Biological systems -- Measurement Cells -- Morphology Saccharomyces cerevisiae -- Effect of stress on Fluorescence microscopy -- Technique NAD (Coenzyme) -- Mechanism of action UCTD Du Plessis, Le Roi Alexander Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title | Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title_full | Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title_fullStr | Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title_full_unstemmed | Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title_short | Integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| title_sort | integrated optical tweezers and microfluidic system for live cell fluorescence spectroscopy to monitor cellular response |
| topic | Optical tweezers Microfluidic devices -- Mathematical models Biological systems -- Measurement Cells -- Morphology Saccharomyces cerevisiae -- Effect of stress on Fluorescence microscopy -- Technique NAD (Coenzyme) -- Mechanism of action UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/132148 |
| work_keys_str_mv | AT duplessisleroialexander integratedopticaltweezersandmicrofluidicsystemforlivecellfluorescencespectroscopytomonitorcellularresponse |