Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Development of biosensor for the early detection of tuberculous meningitis in infants
Kim, D. 2025. Development of biosensor for the early detection of tuberculous meningitis in infants. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/1a4ddd6e-330f-49c3-8f53-8d8591e4ee09
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Stellenbosch : Stellenbosch University
2025
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613771770363904 |
|---|---|
| access_status_str | Open Access |
| author | Kim, Dabin |
| author2 | Perold, W. J. |
| author_browse | Kim, Dabin Perold, W. J. |
| author_facet | Perold, W. J. Kim, Dabin |
| author_sort | Kim, Dabin |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Kim, D. 2025. Development of biosensor for the early detection of tuberculous meningitis in infants. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/1a4ddd6e-330f-49c3-8f53-8d8591e4ee09 |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/132225 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:41:26.849Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/132225 Development of biosensor for the early detection of tuberculous meningitis in infants Kim, Dabin Perold, W. J. Chegou, Novel N. Stellenbosch University. Faculty of Engineering. Dept. of Electrical and Electronic Engineering. Meninges -- Tuberculosis-- Early detection Biosensors Medical care -- Technological innovations Medical instruments and apparatus UCTD Kim, D. 2025. Development of biosensor for the early detection of tuberculous meningitis in infants. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/1a4ddd6e-330f-49c3-8f53-8d8591e4ee09 Thesis (MEng)--Stellenbosch University, 2025. ENGLISH ABSTRACT: Tuberculous meningitis (TBM is a severe illness that is predominantly observed in countries with a high burden of tuberculosis. It is primarily found in infants and HIV‑infected adults and if left untreated, causes irreversible damage to the host’s nerve and brain tissue, often leading to mor‑tality. Current methods of TBM detection rely on cerebrospinal fluid (CSF culture, which may only yield results in up to 6 weeks, is not very sensitive, and requires a biological safety level III laboratory to conduct. Other detection methods are equally not very sensitive and laborious. This research investigates the detection of IFN‑γ protein biomarker through the use of fluoro‑immunoassay with an optical biosensor and a custom‑manufactured chip. The glass‑surface of the chip was treated with 3‑Aminopropyltriethoxysilane and incubated with glutaraldehyde to prepare for immobilization, after which a Sandwich ELISA format was used to perform a dilution series by immobilizing the capture antibody, IFN‑γ protein and FITC stained detection antibody on to the chip. The optical biosensor excited the FITC stained antibodies to capture the emission light at multiple exposures, which were then merged to create a HDR image for image processing. The results from the optical biosensor were verified with a Zeiss LSM780 confocal microscope. The system demonstrated the capability to rapidly identify the biomarker, detect the binding sites, and quantify IFN‑γ in blood serum. This fluorescent optical sensor proposes a possible approach for the development of a point‑of‑care system for TBM, providing a quicker and simpler method for the early detection of TBM. AFRIKAANSE OPSOMMING: Tuberkulose meningitis (TBM is ’n ernstige siekte wat oorwegend waargeneem word in lande met ’n hoë voorkoms van tuberkulose. Dit word hoofsaaklik gevind in babas en IV‑geı̈nfekteerde volwassenes en, indien onbehandel, veroorsaak dit onomkeerbare skade aan die gasheer se senuwee‑ en breinweefsel, wat dikwels tot mortaliteit lei. Huidige metodes van TBM‑opsporing maak staat op serebrospinale vloeistof (SSF kultuur, waar resultate soms eers na 6 weke beskikbaar is, is nie baie sensitief nie, en vereis ’n biologiese veiligheidsvlak III‑laboratorium. Ander opsporingsmetodes is eweneens tydrowend en ook nie baie sensitief nie. Hierdie navorsing ondersoek die opsporing van IFN‑𝛾‑proteı̈en as biomerker, deur die gebruik van ’n luoro‑immunotoets met ’n optiese biosensor en ’n pasgemaakte skyie. Die glasoppervlak van die skyie is behandel met (3‑Aminopropyltriethoxysilaan en geı̈nkubeer met gluteraldehied, ter voorbereiding vir immobilisasie, waarna ’n Toebroodjie ELISA‑formaat gebruik is om ’n verdunningsreeks uit te voer, deur die immobilisasie van opspoor‑teenliggaampies, IFN‑𝛾‑proteı̈en en FITC‑gekleurde opspoor‑teenliggaampies op die skyie. Die optiese biosensor het die emissie van die FITC‑gekleurde teenliggaampies by verskillende beligtings vasgevang, wat dan saamgevoeg is om ’n HDR‑beeld vir beeldverwerking te skep. Die resultate van die optiese biosensor is met ’n Zeiss LSM780 konfokale mikroskoop geveriieer. Die stelsel het die vermoë getoon om vinnige identiisering vand die biomerker te doen, die bindingsplekke waar te neem en om IFN‑𝛾 in bloedserum te kwantiiseer. Hierdie luoreserende optiese sensor stel ’n benadering voor vir die ontwikkeling van ’n punt‑van‑sorg‑stelsel vir TBM, wat potensiëel’n vinniger en eenvoudiger metode bied vir die vroeë opsporing van TBM. Masters 2025-05-30T09:05:25Z 2025-05-30T09:05:25Z 2025-03 Thesis https://scholar.sun.ac.za/handle/10019.1/132225 en Stellenbosch University xiv, 114 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Meninges -- Tuberculosis-- Early detection Biosensors Medical care -- Technological innovations Medical instruments and apparatus UCTD Kim, Dabin Development of biosensor for the early detection of tuberculous meningitis in infants |
| title | Development of biosensor for the early detection of tuberculous meningitis in infants |
| title_full | Development of biosensor for the early detection of tuberculous meningitis in infants |
| title_fullStr | Development of biosensor for the early detection of tuberculous meningitis in infants |
| title_full_unstemmed | Development of biosensor for the early detection of tuberculous meningitis in infants |
| title_short | Development of biosensor for the early detection of tuberculous meningitis in infants |
| title_sort | development of biosensor for the early detection of tuberculous meningitis in infants |
| topic | Meninges -- Tuberculosis-- Early detection Biosensors Medical care -- Technological innovations Medical instruments and apparatus UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/132225 |
| work_keys_str_mv | AT kimdabin developmentofbiosensorfortheearlydetectionoftuberculousmeningitisininfants |