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Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors
Thesis (MEngSci)--Stellenbosch University, 2025.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867614098001231872 |
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| access_status_str | Open Access |
| author | Maharaj, Tarushai |
| author2 | Perold, Willem J. |
| author_browse | Maharaj, Tarushai Perold, Willem J. |
| author_facet | Perold, Willem J. Maharaj, Tarushai |
| author_sort | Maharaj, Tarushai |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MEngSci)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/132234 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:46:38.139Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/132234 Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors Maharaj, Tarushai Perold, Willem J. Engelbrecht, Anna-Mart Stellenbosch University. Faculty of Engineering. Dept. of Mechanical and Mechatronic Engineering. Ovaries -- Cancer -- Diagnosis Biomedical engineering Electrochemical sensors UCTD Thesis (MEngSci)--Stellenbosch University, 2025. Maharaj, T. 2025. Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/dec8f52a-8923-4fa3-aaef-eeaa033dc973 ENGLISH ABSTRACT: Ovarian cancer (OC), famously termed the ‘silent killer’ is the deadliest of the gynaecological cancers that effect women today with a 5% survival prognosis after diagnosis. In addition to this, the incidence of ovarian cancer is projected to rise in Africa by 80% by 2050. The survival prognosis after detection increases to 90% if the disease progression is detected in its early stages. Unfortunately, existing methods of OC detection are not effective in detecting the disease in its early stages where the disease is curable. The available methods that exist for detection of ovarian cancer are also either invasive, time-costly or expensive. Biosensors are point-of-care devices that integrate biologically derived molecules as biorecognition elements with a transducer for the detection of a molecule indicative of disease known as a ‘biomarker’. This project proposes a proof-of-concept study towards the development of a DNA-based electrochemical device for the detection of Heat Shock Protein 10, a novel biomarker of early-stage OC in an attempt to overcome the shortcomings of current OC detectionmethods. A gold screen-printed electrode was chosen as the sensor transducer. Two strategies of immobilising the DNA biorecognition element to the transducer were investigated. The first method exploits the strong affinity between biotin and streptavidin whilst the other exploits the thiol-gold bond. Square Wave Voltammetry was the electrochemical technique chosen to detect the presence of HSP10 on the working electrode of the biosensor. An electrochemical potentiostat was used to establish the response current peaks during SWV analysis. The device was further used to analyse the change in response current peaks as a result of the changes in electrochemical redox reactions at the working electrode as they interact with the biorecognition element after immobilisation. AFRIKAANSE OPSOMMING: Eierstokkanker (EK), berug as die ‘stille moordenaar,’ is die dodelikste van die ginekologiese kankers wat vroue tans tref, met ’n oorlewingsvooruitsig van slegs 5% ná diagnose. Daarbenewens word voorspel dat die voorkoms van eierstokkanker in Afrika teen 2050 met 80% sal toeneem. Die oorlewingsvooruitsig ná diagnose styg tot 90% indien die siekte se vordering in die vroeë stadiums opgespoor word. Ongelukkig is bestaande metodes vir die opsporing van EK nie doeltreffend om die siekte in hierdie vroeë, geneesbare stadiums te identifiseer nie. Die beskikbare opsporingsmetodes vir eierstokkanker is ook óf indringend, tydrowend, óf duur. Biosensors is punt-van-sorg-toestelle wat biologies-afgeleide molekules integreer as bioherkenningselemente met 'n transducer vir die opsporing van 'n molekule wat aandui op siekte bekend as 'n 'biomerker'. Hierdie projek stel ’n bewys-van-konsep studie voor vir die ontwikkeling van ’n DNA-gebaseerde elektrochemiese toestel vir die opsporing van Heat Shock Protein 10 (HSP10), ’n nuwe biomerker van vroeë-stadium eierstokkanker(EK), in ’n poging om die tekortkominge van huidige EK-opsporingsmetodes te oorkom. 'n Goue skermgedrukte elektrode is as die sensor-omskakelaar gekies. Twee strategieë om die DNA-bioherkenningselement na die transducer te immobiliseer is ondersoek. Die eerste metode ontgin die sterk affiniteit tussen biotien en streptavidien, terwyl die ander die tiol-goudbinding ontgin. Vierkant-golf voltammetrie is die elektrochemiese tegniek wat gekies is om die teenwoordigheid van HSP10 op die werkende elektrode van die biosensor op te spoor. ’n Elektrochemiese potensiostaat is gebruik om die reaksie-stroompieks tydens die SWV-analise vas te stel. Die toestel is verder gebruik om die verandering in reaksie-stroompieks te ontleed as gevolg van veranderinge in elektrochemiese redoksreaksies by die werkende elektrode soos wat hulle in wisselwerking met die bioherkenningselement na immobilisering inwerk. Masters 2025-05-30T10:35:35Z 2025-05-30T10:35:35Z 2025-03 Thesis https://scholar.sun.ac.za/handle/10019.1/132234 en_ZA Stellenbosch University [xiv], 89 pages : illustrations, includes annexures application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Ovaries -- Cancer -- Diagnosis Biomedical engineering Electrochemical sensors UCTD Maharaj, Tarushai Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title | Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title_full | Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title_fullStr | Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title_full_unstemmed | Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title_short | Towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker HSP10 and aptamer bioreceptors |
| title_sort | towards the development of an electrochemical aptasensor for the early detection of ovarian cancer using the novel biomarker hsp10 and aptamer bioreceptors |
| topic | Ovaries -- Cancer -- Diagnosis Biomedical engineering Electrochemical sensors UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/132234 |
| work_keys_str_mv | AT maharajtarushai towardsthedevelopmentofanelectrochemicalaptasensorfortheearlydetectionofovariancancerusingthenovelbiomarkerhsp10andaptamerbioreceptors |