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Development and evaluation of a surface plasmon resonance biosensor
Thesis (MEng)--Stellenbosch University, 2025.
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867613941456175104 |
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| access_status_str | Open Access |
| author | Jacobs, Hanri Valkyrie |
| author2 | Perold, Willem J. |
| author_browse | Jacobs, Hanri Valkyrie Perold, Willem J. |
| author_facet | Perold, Willem J. Jacobs, Hanri Valkyrie |
| author_sort | Jacobs, Hanri Valkyrie |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MEng)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/132512 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:44:08.546Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/132512 Development and evaluation of a surface plasmon resonance biosensor Jacobs, Hanri Valkyrie Perold, Willem J. Bosman, Gurthwin W. Engelbrecht, Anna-Mart Stellenbosch University. Faculty of Engineering. Dept. of Mechanical and Mechatronic Engineering. Point-of-care testing Surface plasmon resonance Biosensors Optical detectors UCTD Thesis (MEng)--Stellenbosch University, 2025. Jacobs, H. V. 2025. Development and evaluation of a surface plasmon resonance biosensor. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/841d917f-9284-4d5a-8c67-2e3b83f2583a ENGLISH ABSTRACT: Point-of-care (POC) testing has become increasingly important, highlighting the need for reliable, rapid, low-cost diagnostic tools compared to traditional laboratory testing methods. The early detection of diseases and personalised healthcare have garnered significant attention. Optical biosensors, particularly Surface Plasmon Resonance (SPR) sensors, hold great promise in addressing these challenges due to their label-free nature, cost-effectiveness, consistent results, ability to perform multiplex measurements, and superior robustness, lifespan, and ease of integration for POC applications. A novel SPR biosensor was developed as a proof of concept, building on a previous Master’s project at Stellenbosch University. This project addresses critical challenges in designing and developing SPR biosensors identified in the earlier work, filling gaps in fundamental theories necessary for building a viable POC sensor. Simulation methods were assessed to identify the most appropriate approach for the project, and key sensor parameters were analysed to determine optimal responses. Additionally, the performance of a dual-sensing system, combining SPR and impedance spectroscopy, was simulated and investigated using an interdigitated electrode (IDE) with COMSOL Multiphysics. The experimental setup for SPR angular interrogation was developed by consideringa range of design factors and employing a cost-effective method for beam-angle scanning, utilising a Piezo motor-enabled slit scanning system. A data processing and acquisition system was developed to control the Piezo-motor movements and data sampling. The biosensor was then characterised, laying the groundwork for its application in biological sensing. The project concluded with a thorough evaluation of the system’s ability to detect changes in the concentration of binary mixtures and a model biomarker. Both wide range and high-resolution refractive index measurements were conducted. The results demonstrated that the sensor could detect ethanol in concentrations of 394.5 ng/μL with a resolution of 7.286 × 10−5RIU. Bovine Serum Albumin (BSA) results show that the sensor can detect low concentrations of 2 μg/μL and promise high sensitivity for detecting biomarkers. This proof-of-concept SPR biosensor can be developed into a sensitive and functional enhanced POC testing tool. Potential improvements include merging the biosensor with interdigitated electrodes for dual-parameter sensing, integrating microfluidics to better account for environmental factors and examining specific biomarkers, combining the biosensor with advancements such as localised surface plasmon resonance for improved sensitivity, incorporating high-accuracy Piezo motors or alternatives such as digital micromirror devices to increase scanning speed, and miniaturising the experimental setup. AFRIKAANSE OPSOMMING: Punt-van-sorg (POC) toetsing het al hoe belangriker geword, wat die behoefte aan betroubare, vinnige, laekoste diagnostiese instrumente beklemtoon in vergelyking met tradisionele laboratorium toetsmetodes. Die vroeë opsporing van siektes en persoonlike gesondheidsorg het aansienlike aandag begin kry. Optiese biosensors, spesifiek Oppervlakte Plasmon Resonansie (SPR) sensors, hou groot belofte in om hierdie uitdagings aan te spreek vanweë hulle etiketvrye aard, koste-effektiwiteit, konsekwente resultate, vermoë om multipleksmetings uit te voer, en voortreflike robuustheid, lewensduur en gemak van integrasie vir POC toepassings. ’n Innoverende SPR-biosensor is ontwikkel as ’n bewys-van-konsep wat voortbou op ’n vorige Meestersgraad projek aan die Universiteit van Stellenbosch. Hierdie projek spreek kritieke uitdagings aan in die ontwerp en ontwikkeling van SPR-biosensors wat in die vorige werk geïdentifiseer is, en vul leemtes in fundamentele teorieë wat nodig is vir die bou van ’n lewensvatbare POCsensor. Simulasiemetodes is geassesseer om die mees geskikte benadering vir die projek te identifiseer, en sleutel sensor parameters is ontleed om optimale response te bepaal. Daarbenewens is die werkverrigting van ’n dubbelwaarnemingstelsel, wat SPR en impedansiespektroskopie kombineer, gesimuleer en ondersoek met behulp van ’n interdigitale elektrode (IDE) met COMSOL Multiphysics. Die eksperimentele opstelling vir SPR hoekverandering is ontwikkel deur ’n reeks ontwerpfaktore in ag te neem en ’n koste-effektiewe metode vir straalhoekskandering te gebruik met behulp van ’n Piezo motor-geaktiveerdespleetskanderingstelsel. ’n Dataverwerking en -verkrygingstelsel is ontwikkel om die Piezo-motoriese bewegings en datamonsterneming te beheer. Die biosensor is toe gekarakteriseer, wat die grondslag gelê het vir die toepassing daarvan in biologiese waarneming. Die projek is afgesluit met ’n deeglike evaluering van die stelsel se vermoë om veranderinge in die konsentrasie van binêre mengsels en ’n model biomerker op te spoor. Beide wye reeks en hoë resolusie brekingsindeksmetings is uitgevoer. Die resultate het getoon dat die sensor etanol kon opspoor in konsentrasies van 394.5 ng/μL met ’n resolusie van 7.286×10−5RIU. Bees Serum Albumien (BSA) resultate toon dat die sensor lae konsentrasies van 2 μg/μL kan opspoor en beloof hoë sensitiwiteit vir die opsporing van biomerkers. Hierdie bewys-van-konsep SPR-biosensor kan ontwikkel word tot ’n sensitiewe en funksionele verbeterde POC-toetsinstrument. Potensiële verbeterings sluit in die samevoeging van die biosensor met interdigitale elektrodes vir dubbelparameter-waarneming, die integrasie van mikrovloeistowwe om beter rekening te hou met omgewingsfaktore en die ondersoek van spesifieke biomerkers, die kombinasie van die biosensor met vooruitgang soos gelokaliseerde oppervlak plasmon resonansie vir verbeterde sensitiwiteit, die inkorporering van hoë-akkurate Piezo motors of alternatiewe soos digitale mikrospieëltoestelle om skanderingspoed te verhoog, en die minituarisering van die eksperimentele opstelling. Masters 2025-06-10T10:14:16Z 2025-06-10T10:14:16Z 2025-03 Thesis https://scholar.sun.ac.za/handle/10019.1/132512 en_ZA Stellenbosch University xvi, 98 pages : illustrations, includes annexures application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Point-of-care testing Surface plasmon resonance Biosensors Optical detectors UCTD Jacobs, Hanri Valkyrie Development and evaluation of a surface plasmon resonance biosensor |
| title | Development and evaluation of a surface plasmon resonance biosensor |
| title_full | Development and evaluation of a surface plasmon resonance biosensor |
| title_fullStr | Development and evaluation of a surface plasmon resonance biosensor |
| title_full_unstemmed | Development and evaluation of a surface plasmon resonance biosensor |
| title_short | Development and evaluation of a surface plasmon resonance biosensor |
| title_sort | development and evaluation of a surface plasmon resonance biosensor |
| topic | Point-of-care testing Surface plasmon resonance Biosensors Optical detectors UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/132512 |
| work_keys_str_mv | AT jacobshanrivalkyrie developmentandevaluationofasurfaceplasmonresonancebiosensor |