Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
Thesis (MEngSc)--Stellenbosch University, 2025.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
| Published: |
Stellenbosch : Stellenbosch University
2025
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613836651003904 |
|---|---|
| access_status_str | Open Access |
| author | Osman, Ridwaan |
| author2 | Rambharose, Sanjeev |
| author_browse | Osman, Ridwaan Rambharose, Sanjeev |
| author_facet | Rambharose, Sanjeev Osman, Ridwaan |
| author_sort | Osman, Ridwaan |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MEngSc)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/132520 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:42:28.529Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/132520 In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines Osman, Ridwaan Rambharose, Sanjeev Engelbrecht, Anna-Mart Perold, Willem J. Stellenbosch University. Faculty of Engineering. Institute of Biomedical Engineering. Breast -- Cancer -- Alternative treatment Nanomedicine Nanotechnology UCTD Thesis (MEngSc)--Stellenbosch University, 2025. Osman, R. 2025. In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/3e73c379-3b25-48c5-b51e-7e3f8bfe704c ENGLISH ABSTRACT: Breast cancer is the most common cancer in both South Africa and globally. Despite significant advancements in cancer therapy over the past century, challenges in treatment efficacy remain, requiring novel solutions with fewer side effects. Nanotechnology offers promise, particularly gold nanoparticles (GNPs), due to their stability, ease of functionalisation, and low cytotoxicity. However, traditional GNP synthesis is hindered by high production costs and toxic by-products. Green synthesis has emerged as an eco-friendly, cost-effective alternative for producing green gold nanoparticles (GGNPs). This study utilised Tannic Acid, Gallic Acid, and Quercetin—plant-derived compounds with known anticancer activity but poor solubility and rapid clearance—as reducing and capping agents in the synthesis of GGNPs. The formation of nanoparticles was confirmed by a colour change and UV-visible spectroscopy, with absorbance peaks at 515 nm, 555 nm, and 562 nm. Dynamic light scattering (DLS) revealed particle sizes of 86 nm, 32 nm, and 112 nm, with polydispersity indices (PDI) of 0.35, 0.31, and 0.16, respectively. Zeta potentials of -41, -37, and -47 mV confirmed sample stability, which was maintained over three months at room temperature and 4°C. GGNPs were further characterised with Scanning Electron Microscopy and Fourier Transform Infrared Spectroscopy. Each GGNP exhibited antioxidant and anticancer activity, which was tested against MDA-MB-231 and MCF-7 breast cancer cell lines, as well as normal breast epithelial MCF-12 cells. This study highlights the potential of GGNPs as a targeted, eco-friendly therapy for breast cancer. AFRIKAANSE OPSOMMING: Borskanker is die mees algemene kanker in Suid-Afrika sowel as wêreldwyd. Ten spyte van vooruitgang in kankerterapie oor die afgelope eeu, bly daar uitdagings in die doeltreffende behandeling daarvan, wat nuwe oplossings met minder newe-effekte vereis. Nanotegnologie bied hoop, veral goue nanopartikels (GNP's), in terme van hul stabiliteit, gemak van bruikbaarheid en lae sitotoksisiteit. Tradisionele GNP-sintese word egter beperk deur hoë produksiekoste en toksiese byprodukte. Groen sintese het ontstaan as 'n omgewingsvriendelike, koste doeltreffende alternatief vir die vervaardiging van groen-goue nanopartikels (GGNP's). In studie is Tanniensuur, Galliensuur en Quercetin gebruik—plantgebaseerde verbindings met bekende anti-kankeraktiwiteit, maar met swak oplosbaarheid en vinnige uitskeiding—om as reduseer- en stabiliseermiddel in die sintese van GGNP's te dien. Die vorming van nanopartikels is bevestig deur 'n kleurverandering en UV-sigbare spektroskopie, met absorpsie pieke by 515 nm, 555 nm en 562 nm. Dinamiese ligverstrooiing (DLS) het deeltjiegroottes van 86 nm, 32 nm en 112 nm aangetoon, met polidispersiteitsindekse (PDI) van onderskeidelik 0.35, 0.31 en 0.16. Zeta-potensiale van -41, -37 en -47 mV het monster stabiliteit bevestig, wat oor ‘n tydperk van drie maande by kamertemperatuur en 4°C gehandhaaf is. GGNP's is verder gekarakteriseer met skandeerelektronmikroskopie en Fourier-transform Infrarooi Spektroskopie. Die GGNP het antioksidant- en anti-kankeraktiwiteit getoon, wat in MDA-MB-231 en MCF-7 borskankersellyne, sowel as normale bors-epiteelselle, MCF-12, getoets is. Hierdie studie beklemtoon die potensiaal van GGNP's as 'n geteikende, omgewingsvriendelike terapie vir borskanker. Masters 2025-06-10T12:06:30Z 2025-06-10T12:06:30Z 2025-03 Thesis https://scholar.sun.ac.za/handle/10019.1/132520 en_ZA Stellenbosch University xii, 75 pages : illustrations, includes annexures application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Breast -- Cancer -- Alternative treatment Nanomedicine Nanotechnology UCTD Osman, Ridwaan In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines |
| title | In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
|
| title_full | In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
|
| title_fullStr | In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
|
| title_full_unstemmed | In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
|
| title_short | In vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines
|
| title_sort | in vitro evaluation of the anticancer effects of green gold nanoparticles in breast cancer cell lines |
| topic | Breast -- Cancer -- Alternative treatment Nanomedicine Nanotechnology UCTD |
| url | https://scholar.sun.ac.za/handle/10019.1/132520 |
| work_keys_str_mv | AT osmanridwaan invitroevaluationoftheanticancereffectsofgreengoldnanoparticlesinbreastcancercelllines |