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Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke
Thesis (MSc)--Stellenbosch University, 2025.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2025
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| _version_ | 1867614093476626432 |
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| access_status_str | Open Access |
| author | Mfisa, Kabelo |
| author2 | Petersen-Ross, Kelly |
| author_browse | Mfisa, Kabelo Petersen-Ross, Kelly |
| author_facet | Petersen-Ross, Kelly Mfisa, Kabelo |
| author_sort | Mfisa, Kabelo |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/134704 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:46:33.531Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/134704 Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke Mfisa, Kabelo Petersen-Ross, Kelly Smith, Carine Stellenbosch University. Faculty of Medicine and Health Sciences. Dept. of Biomedical Sciences. Division of Medical Physiology. Stroke -- Treatment Cerebral ischemia Plant extracts -- Therapeutic use Grapes -- Therapeutic use Neuroprotective agents Thesis (MSc)--Stellenbosch University, 2025. Mfisa, K. 2025. Potential Neuroprotective Effects of a Grape Seed-Derived Polyphenol Extract via Modulation of Redox Signaling in the Context of Ischaemic Stroke. Unpublished masters thesis. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/dad5b1ee-9d72-4183-922c-83c3f13fa960 ENGLISH ABSTRACT: Background: Stroke is a cerebrovascular disorder characterised by the disruption of the cerebral blood flow in the brain, leading to neuronal damage and functional impairments. Ischaemic stroke accounts for approximately 65% of all strokes globally, with oxidative stress playing a central role in the neurological damage that occurs during its pathophysiology. Current emergency stroke treatments are aimed at addressing blood reperfusion via anticoagulant treatments, which may not address the neurological deficits resulting from the ischaemia-associated oxidative damage to neurons themselves. This may contribute to poor functional outcomes and long-term physical impairments. It is thus imperative to address oxidative stress at the neuronal level in this context. Previous studies in our group have demonstrated the potential of a grape seed extract (GSE, Oxiprovin™) to attenuate oxidative stress and inflammation, positioning it as a potential candidate drug to further improve outcomes in the context of ischaemic stroke. Aim: The current study explores the potential effects of GSE in modulating redox status, oxidative stress-associated neuronal damage and functional outcome in larval zebrafish subjected to ponatinib-induced ischaemic stroke. Methods: Zebrafish larvae (2dpf) were exposed to ponatinib to induce ischaemic stroke, in the absence or presence of a dose range of the GSE. The functional effect of the GSE on the stroke-induced zebrafish model was assessed, employing both acute and preventative treatment regimens. Both interventions were administered by immersion. Immediate neuronal damage was assessed at 2dpf and during recovery at 4dpf, while redox status and functional recovery (general activity levels) were assessed at 4dpf. Results: Ponatinib-exposed zebrafish larvae exhibited a significant decrease in basal locomotive activity when compared to the groups co-treated with the GSE. Both the acute and preventive treatment regimens improved behavioral functions with a greater recovery observed in the acutely treated larvae. Neuronal molecular markers NeuroD1 and TH1 indicated that post-stroke treatment with GSE limited neuronal damage with both preventative and acute treatments preserving both markers, however, the acute treatment showed a better recovery. Redox testing illustrated elevated levels of free radicals in the Ponatinib-exposed group, which GSE effectively mitigated when administered both acutely and preventatively. Conclusion: The ability of GSE to reduce oxidative stress-related neuronal damage in this preclinical stroke model warrants its further development as a complementary treatment in ischaemic stroke. AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar. Masters 2025-12-24T08:37:48Z 2025-12-24T08:37:48Z 2025-12 Thesis https://scholar.sun.ac.za/handle/10019.1/134704 en Stellenbosch University x, 65 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Stroke -- Treatment Cerebral ischemia Plant extracts -- Therapeutic use Grapes -- Therapeutic use Neuroprotective agents Mfisa, Kabelo Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title | Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title_full | Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title_fullStr | Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title_full_unstemmed | Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title_short | Potential neuroprotective effects of a grape seed-derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| title_sort | potential neuroprotective effects of a grape seed derived polyphenol extract via modulation of redox signaling in the context of ischaemic stroke |
| topic | Stroke -- Treatment Cerebral ischemia Plant extracts -- Therapeutic use Grapes -- Therapeutic use Neuroprotective agents |
| url | https://scholar.sun.ac.za/handle/10019.1/134704 |
| work_keys_str_mv | AT mfisakabelo potentialneuroprotectiveeffectsofagrapeseedderivedpolyphenolextractviamodulationofredoxsignalinginthecontextofischaemicstroke |