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Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms
Thesis (PhD)--Stellenbosch University, 2025.
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Stellenbosch : Stellenbosch University
2026
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| _version_ | 1867613736432304128 |
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| access_status_str | Open Access |
| author | Noach, Matthew Nigel |
| author2 | Goosen, Neill |
| author_browse | Goosen, Neill Noach, Matthew Nigel |
| author_facet | Goosen, Neill Noach, Matthew Nigel |
| author_sort | Noach, Matthew Nigel |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (PhD)--Stellenbosch University, 2025. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/134746 |
| institution | Stellenbosch University (South Africa) |
| last_indexed | 2026-06-10T12:40:53.123Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/134746 Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms Noach, Matthew Nigel Goosen, Neill Pott, Robbie Van Rensburg, Eugéne Stellenbosch University. Faculty of Engineering. Dept. of Chemical Engineering. Marine algae -- Biotechnology Microbial enzymes Biochemical engineering Haliotis midae Thesis (PhD)--Stellenbosch University, 2025. Noach, M. N. 2025. Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms. Unpublished doctoral dissertation. Stellenbosch: Stellenbosch University [online]. Available: https://scholar.sun.ac.za/items/03a1876f-48c3-445c-938b-31bfdd885cf8 ENGLISH ABTRACT: The world is gradually moving toward utilising the abundant resources found within the marine environment to develop new and beneficial products. Generally, brown seaweeds are mainly composed of alginate. Enzymatic treatment of alginate by alginate lyases (ALs) yields low molecular weight (MW) products known as alginate oligosaccharides (AOs). AOs have received growing interest due to the inherent bioactivity, which may be exploited in various sectors. Enzymatic treatment of brown seaweeds with AL is a valuable process for the production of AOs, and the release of other contained cellular components. Microbial fermentation of AL producing microbes is particularly appealing since it requires a single process step, and negates the need to harvest enzyme and perform a separate reaction step. However, at present there has been little development of microbial fermentation bioprocess utilising wild-type AL producing microorganisms. Particularly those which utilise Ecklonia maxima as a substrate. The aim of this study was to develop a microbial fermentation process for the direct production of alginate oligosaccharides from Ecklonia maxima using isolated wild-type AL producing microorganisms. This was achieved by (i) isolation and identification of extracellular AL producing microorganisms from environmental sources, (ii) investigating the effect of medium composition and fermentation conditions on growth and enzyme production of select microorganisms, (iii) the direct production of AOs from brown seaweeds by Rhodotorula sp., (iv) and the impact of alginate concentration under elevated salt conditions on fermentation conditions in stirred bioreactors. AFRIKAANSE OPSOMMING: Geen opsomming beskikbaar. Doctoral 2026-01-06T12:07:24Z 2026-01-06T12:07:24Z 2025-12 Thesis https://scholar.sun.ac.za/handle/10019.1/134746 Stellenbosch University xx, 279 pages : illustrations application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Marine algae -- Biotechnology Microbial enzymes Biochemical engineering Haliotis midae Noach, Matthew Nigel Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title | Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title_full | Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title_fullStr | Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title_full_unstemmed | Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title_short | Development of a microbial bioprocess for enzymatic breakdown of Ecklonia maxima using isolated microorganisms |
| title_sort | development of a microbial bioprocess for enzymatic breakdown of ecklonia maxima using isolated microorganisms |
| topic | Marine algae -- Biotechnology Microbial enzymes Biochemical engineering Haliotis midae |
| url | https://scholar.sun.ac.za/handle/10019.1/134746 |
| work_keys_str_mv | AT noachmatthewnigel developmentofamicrobialbioprocessforenzymaticbreakdownofeckloniamaximausingisolatedmicroorganisms |