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A biochemical and immunological study of horseradish peroxidase
Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2009
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| _version_ | 1867613957709103104 |
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| access_status_str | Open Access |
| author | Odendaal, Ruenda |
| author2 | Swart, P. |
| author_browse | Odendaal, Ruenda Swart, P. |
| author_facet | Swart, P. Odendaal, Ruenda |
| author_sort | Odendaal, Ruenda |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/1587 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:24.378Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/1587 A biochemical and immunological study of horseradish peroxidase Odendaal, Ruenda Swart, P. Lombard, N. University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. Horseradish peroxidase Isoelectric focusing HPLC Dissertations -- Biochemistry Theses -- Biochemistry Peroxidase -- Analysis Isoensymes Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. ENGLISH ABSTRACT: This study describes: a) the isolation and purification of horseradish peroxidase isoenzymes from horseradish roots, b) the characterization of various forms and components of the enzyme by cation-exchange and reversed-phase high performance liquid chromatography, c) the preparation of antibodies against horseradish peroxidase isoenzymes, d) immunological studies for the development of an isoenzyme quantification method and e) the formation of an enzyme-melamine conjugate for use in a melamine quantification immunoassay. AFRIKAANSE OPSOMMING: Hierdie studie beskryf: a) die isolering en suiwering van peperwortel-peroksidase-isoënsieme vanuit die peperwortel, b) die karakterisering van verskillende vorme en komponente van dié ensiem deur katioonuitruilings en omgekeerde-fase HPLC c) die voorbereiding van teenliggaampies vir peperwortel-peroksidase-isoënsieme, d) immunologiese studies vir die ontwikkeling van 'n isoënsiem-kwantifiseringsmetode; en e) die vorming van 'n ensiem-melamien-konjugaat vir gebruik in 'n melamienkwantifiseringsmetode. 2009-11-24T06:29:22Z 2010-06-01T08:27:59Z 2009-11-24T06:29:22Z 2010-06-01T08:27:59Z 2009-12 Thesis http://hdl.handle.net/10019.1/1587 en University of Stellenbosch application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Horseradish peroxidase Isoelectric focusing HPLC Dissertations -- Biochemistry Theses -- Biochemistry Peroxidase -- Analysis Isoensymes Odendaal, Ruenda A biochemical and immunological study of horseradish peroxidase |
| title | A biochemical and immunological study of horseradish peroxidase |
| title_full | A biochemical and immunological study of horseradish peroxidase |
| title_fullStr | A biochemical and immunological study of horseradish peroxidase |
| title_full_unstemmed | A biochemical and immunological study of horseradish peroxidase |
| title_short | A biochemical and immunological study of horseradish peroxidase |
| title_sort | biochemical and immunological study of horseradish peroxidase |
| topic | Horseradish peroxidase Isoelectric focusing HPLC Dissertations -- Biochemistry Theses -- Biochemistry Peroxidase -- Analysis Isoensymes |
| url | http://hdl.handle.net/10019.1/1587 |
| work_keys_str_mv | AT odendaalruenda abiochemicalandimmunologicalstudyofhorseradishperoxidase AT odendaalruenda biochemicalandimmunologicalstudyofhorseradishperoxidase |