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Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f.
Thesis (MSc (Microbiology))--University of Stellenbosch, 2005.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : University of Stellenbosch
2006
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| _version_ | 1867613768328937472 |
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| access_status_str | Open Access |
| author | Goldschmidt, Gunther Karl |
| author2 | Rawlings, D. E. |
| author_browse | Goldschmidt, Gunther Karl Rawlings, D. E. |
| author_facet | Rawlings, D. E. Goldschmidt, Gunther Karl |
| author_sort | Goldschmidt, Gunther Karl |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Microbiology))--University of Stellenbosch, 2005. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/1588 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:23.238Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/1588 Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. Goldschmidt, Gunther Karl Rawlings, D. E. University of Stellenbosch. Faculty of Science. Dept. of Microbiology. Theses -- Microbiology Dissertations -- Microbiology Minerals -- Biotechnology Plasmids -- Genetics Mines and mineral resources Bacterial leaching Microbiology Thesis (MSc (Microbiology))--University of Stellenbosch, 2005. Plasmid pTC-F14 is a 14.2kb promiscuous, broad-host range IncQ-like mobilizable plasmid isolated from Acidithiobacillus caldus f. At. caldus is a member of a consortium of bacteria (along with Acidithiobacillus ferrooxidans and Leptospirilum ferrooxidans) that is used industrially for decomposing metal sulphide ores and concentrates at temperatures of 40ºC or below which is now a well-established industrial process to recover metals from certain copper, uranium and gold-bearing minerals or mineral concentrates. These biomining microbes are usually obligately acidophilic, autotrophic, usually aerobic iron- or sulphur-oxidizing chemolithotrophic bacteria. Their remarkable physiology allows them to inhabit an ecological niche that is largely inorganic and differs from those environments populated by the more commonly studied non-acidophilic heterotrophic bacteria. At. caldus, is a moderately thermophilic (45 to 50ºC), highly acidophilic (pH1.5 to 2.5) sulphur-oxidizing bacterium, and its role as one of the major players in the industrial decomposition of metal sulphide ores has become evident in recent years. At. caldus f from which pTC-F14 was isolated was found to be one of two dominant organisms in a bacterial consortium undergoing pilot-scale testing for the commercial extraction of nickel from ores. 2006-11-16T08:31:08Z 2010-06-01T08:28:00Z 2006-11-16T08:31:08Z 2010-06-01T08:28:00Z 2005-03 Thesis http://hdl.handle.net/10019.1/1588 en_ZA University of Stellenbosch 1918449 bytes 119454 bytes application/pdf application/pdf application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Theses -- Microbiology Dissertations -- Microbiology Minerals -- Biotechnology Plasmids -- Genetics Mines and mineral resources Bacterial leaching Microbiology Goldschmidt, Gunther Karl Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title | Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title_full | Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title_fullStr | Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title_full_unstemmed | Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title_short | Cloning, Sequencing and Partial Characterization of the Accessory Gene Region of Plasmid pTC-F14 isolated from the Biomining Bacterium Acidithiobacillus caldus f. |
| title_sort | cloning sequencing and partial characterization of the accessory gene region of plasmid ptc f14 isolated from the biomining bacterium acidithiobacillus caldus f |
| topic | Theses -- Microbiology Dissertations -- Microbiology Minerals -- Biotechnology Plasmids -- Genetics Mines and mineral resources Bacterial leaching Microbiology |
| url | http://hdl.handle.net/10019.1/1588 |
| work_keys_str_mv | AT goldschmidtguntherkarl cloningsequencingandpartialcharacterizationoftheaccessorygeneregionofplasmidptcf14isolatedfromthebiominingbacteriumacidithiobacilluscaldusf |