Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
The role of the cumulus oophorus complex during spermatozoa capacitational events
Thesis (MScMedSc (Obstetrics and Gynaecology))--University of Stellenbosch, 2005.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Stellenbosch : University of Stellenbosch
2008
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613973893873664 |
|---|---|
| access_status_str | Open Access |
| author | Rijsdijk, Michelle |
| author2 | Franken, Daniel R. |
| author_browse | Franken, Daniel R. Rijsdijk, Michelle |
| author_facet | Franken, Daniel R. Rijsdijk, Michelle |
| author_sort | Rijsdijk, Michelle |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MScMedSc (Obstetrics and Gynaecology))--University of Stellenbosch, 2005. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/1607 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:39.798Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/1607 The role of the cumulus oophorus complex during spermatozoa capacitational events Rijsdijk, Michelle Franken, Daniel R. University of Stellenbosch. Faculty of Health Sciences. Dept. of Obstetrics and Gynaecology. Spermatogenesis Spermatozoa -- Motility Semen -- Analysis Infertility Fertilization in vitro, Human Spermatozoa transport Dissertations -- Medicine Theses -- Medicine Dissertations -- Obstetrics and gynaecology Theses -- Obstetrics and gynaecology Thesis (MScMedSc (Obstetrics and Gynaecology))--University of Stellenbosch, 2005. Chapter 1 contains a review dealing with nuclear and morphological changes during spermatogenesis and spermatozoa transport with emphasis on the maturation of spermatozoa, capacitation, acrosome reaction and the interaction with the cumulus oophorus complex (COC). The oocyte and cumulus oophorus complex is also discussed particularly on the topic of maturity (oocyte and cumulus maturity). Also presented is a review of the fluorescent binding agents, namely Fluorescein Isothiocyanate labeled with Pisum sativum (FITC-PSA), Chlorotetracycline test (CTC) and Chromomycin A3 (CMA3). Chapter II describes all the materials and methods used during this study. Routine semen analysis is described with emphasis on normal spermatozoon morphology according to strict criteria. The evaluation of capacitation and acrosome reaction (AR) using the CTC and PSA-FITC staining methods as well as the evaluation of spermatozoon nuclear chromatin packaging using the CMA3 staining method is described. Chapter III represents the results recorded in this study. Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to be capacitated and acrosome reacted, with a distinct increase in chromatin packaging quality. A general discussion of the results and future applications are discussed in Chapter IV. In short An in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions and to assist in the selection of functional sperm for intracytoplasmic sperm injection therapy. All relevant references are presented in Chapter V . 2008-08-12T09:37:32Z 2010-06-01T08:28:30Z 2008-08-12T09:37:32Z 2010-06-01T08:28:30Z 2005-12 Thesis http://hdl.handle.net/10019.1/1607 en University of Stellenbosch application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Spermatogenesis Spermatozoa -- Motility Semen -- Analysis Infertility Fertilization in vitro, Human Spermatozoa transport Dissertations -- Medicine Theses -- Medicine Dissertations -- Obstetrics and gynaecology Theses -- Obstetrics and gynaecology Rijsdijk, Michelle The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title | The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title_full | The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title_fullStr | The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title_full_unstemmed | The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title_short | The role of the cumulus oophorus complex during spermatozoa capacitational events |
| title_sort | role of the cumulus oophorus complex during spermatozoa capacitational events |
| topic | Spermatogenesis Spermatozoa -- Motility Semen -- Analysis Infertility Fertilization in vitro, Human Spermatozoa transport Dissertations -- Medicine Theses -- Medicine Dissertations -- Obstetrics and gynaecology Theses -- Obstetrics and gynaecology |
| url | http://hdl.handle.net/10019.1/1607 |
| work_keys_str_mv | AT rijsdijkmichelle theroleofthecumulusoophoruscomplexduringspermatozoacapacitationalevents AT rijsdijkmichelle roleofthecumulusoophoruscomplexduringspermatozoacapacitationalevents |