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Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis
Assignment (MMed)--University of Stellenbosch, 2007.
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| Other Authors: | |
| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2011
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| _version_ | 1867614105257377792 |
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| access_status_str | Open Access |
| author | Le Riche, Mia |
| author2 | Marias, David |
| author_browse | Le Riche, Mia Marias, David |
| author_facet | Marias, David Le Riche, Mia |
| author_sort | Le Riche, Mia |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Assignment (MMed)--University of Stellenbosch, 2007. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/18218 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:46:44.579Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/18218 Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis Le Riche, Mia Marias, David Erasmus, Rajiv T. Stellenbosch University. Faculty of Health Sciences. Dept. of Pathology. Chemical Pathology. Lipoproteins -- Metabolism Biochemistry Polyacrylamide gel electrophoresis Lipoprotein X(LpX) Theses -- Medicine Dissertations -- Medicine Assignment (MMed)--University of Stellenbosch, 2007. ENGLISH ABSTRACT: Lipoprotein X (LpX) is an abnormal cholesterol-containing particle that may be present in the serum of subjects with cholestasis, lecithin:cholesterol acyltransferase (LCAT) deficiency and parenteral nutrition. The biochemistry, metabolism, clinical significance and laboratory analysis of LpX is discussed in this study. This laboratory-based project investigated icteric samples received at the Chemical Pathology laboratory, Tygerberg Hospital, for serum predictors of LpX and the use of a modified non-denaturing polyacrylamide gradient gel electrophoresis system in the detection of LpX. The study showed that the non-denaturing polyacrylamide gradient gel electrophoresis system (2-8%) is a useful test in demonstrating LpX in icteric plasma and has potential for a screening test in LCAT deficiency. Serum concentration of conjugated bilirubin, alkaline phosphatase, gamma glutamyltransferase, free cholesterol, phospholipid, free cholesterol: total cholesterol ratio and conjugated bilirubin: total bilirubin ratio are all good predictors of LpX. The ratio of free cholesterol to total cholesterol (FC/TC > 0.6) was the best predictor of LpX. In the setting of obstructive liver disease LpX is seen in 66% of patients if total cholesterol is > 7.5 mmol/L. AFRIKAANSE OPSOMMING: Lipoproteien X (LpX) is ‘n abnormale cholesterol-bevattende partikel wat teenwoordig mag wees in die serum van persone met cholestase, lesitien:cholesterol asieltransferase (LCAT) gebrek en parenterale voeding. Die biochemie, metabolisme, kliniese belang en laboratorium analise van LpX word bespreek in hierdie werkstuk. Hierdie laboratorium-gebaseerde projek het geelsugtige monsters ondersoek wat ontvang is by die Chemiese Patologie laboratorium, Tygerberg Hospitaal, vir serum voorspellers van LpX en die gebruik van ‘n gemodifiseerde nie-denaturerende polie-akrielamied gradiënt gel elektroforese sisteem in die demonstrasie van LpX. Die bevindinge was dat die nie-denaturerende polie-akrielamied gradient gel elektroforese sisteem (2-8%) is ‘n nuttige toets om LpX te demonstreer in geelsugtige plasma en het potensiaal as ‘n siftingstoets in LCAT gebrek. Serum konsentrasie van gekonjugeerde bilirubien, alkaliese fosfatase, gamma glutamieltransferase, vry cholesterol, fosfolipied, vry cholesterol:totale cholesterol verhouding en gekonjugeerde bilirubien:totale bilirubien verhouding is alles goeie voorspellers van LpX. Die verhouding van vry cholesterol tot totale cholesterol (VC/TC > 0.6) was die beste voorspeller van LpX. In gevalle van obstruktiewe lewersiekte word LpX gesien in 66% van pasiente as die totale cholesterol meer as 7.5 mmol/l is. 2011-12-15T10:06:28Z 2011-12-15T10:06:28Z 2007-12 Thesis http://hdl.handle.net/10019.1/18218 en_ZA Stellenbosch University 87 leaves : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Lipoproteins -- Metabolism Biochemistry Polyacrylamide gel electrophoresis Lipoprotein X(LpX) Theses -- Medicine Dissertations -- Medicine Le Riche, Mia Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title | Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title_full | Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title_fullStr | Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title_full_unstemmed | Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title_short | Lipoprotein X : biochemical predictors and detection by non-denaturing polyacrylamide gradient gel electrophoresis |
| title_sort | lipoprotein x biochemical predictors and detection by non denaturing polyacrylamide gradient gel electrophoresis |
| topic | Lipoproteins -- Metabolism Biochemistry Polyacrylamide gel electrophoresis Lipoprotein X(LpX) Theses -- Medicine Dissertations -- Medicine |
| url | http://hdl.handle.net/10019.1/18218 |
| work_keys_str_mv | AT lerichemia lipoproteinxbiochemicalpredictorsanddetectionbynondenaturingpolyacrylamidegradientgelelectrophoresis |