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Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules
Thesis (MSc)--Stellenbosch University, 2012.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613760128024576 |
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| access_status_str | Open Access |
| author | Jacobs, Frans Alexander |
| author2 | Ludidi, N. N. |
| author_browse | Jacobs, Frans Alexander Ludidi, N. N. |
| author_facet | Ludidi, N. N. Jacobs, Frans Alexander |
| author_sort | Jacobs, Frans Alexander |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2012. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/20024 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:41:15.521Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/20024 Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules Jacobs, Frans Alexander Ludidi, N. N. Hills, Paul N. Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Institute for Plant Biotechnology SOD Superoxide dismutase Nitric oxide Cystatin Salt stress in plants Soybean Nodules Dissertations -- Plant biotechnology Theses -- Plant biotechnology Dissertations -- Genetics Theses -- Genetics Thesis (MSc)--Stellenbosch University, 2012. ENGLISH ABSTRACT: Nitric oxide (NO) has previously been shown to play a vital role in plants that are undergoing oxidative stress arising from abiotic stress. To better understand the role of NO on the antioxidative pathway, the effect of NO on Superoxide Dismutase (SOD) activity was studied during salt stress on soybean nodules. The enzymatic activity of specific MnSOD and FeSOD isoforms increased upon 1 week of exposure of nodules to NO or salt stress, the activity of CuZnSOD isoforms however increased in response to salt stress only. Furthermore, 4 putative FeSOD and MnSOD transcripts were identified and shown to increase in response to NO and salt stress. The promoter sequences of these NO-responsive putative SOD genes were analysed alongside a cystatin (AtCYS-1) which is also NO-inducible. Putative NO-responsive cis-acting elements as well as abiotic stress-responsive cis-acting elements were studied amongst these promoter sequences. The MYCL element and the AtMYB4 binding site were found to occur in all four NO-inducible SOD promoter sequences as well as in the AtCYS-1 promoter sequence. This suggests that NO acts via MYCL and/or AtMYB4 to up-regulate specific FeSODs and MnSODs, causing an increase in the activity of these SOD isoforms, thus reducing oxidative stress and cell death in soybean nodules. Furthermore, NO may also be up-regulating cystatins to inhibit cysteine proteases, thus preventing the onset of programmed cell death (PCD) and subsequently reducing salt stress-induced cell death. AFRIKAANSE OPSOMMING: Geen opsomming Masters 2012-01-23T09:24:33Z 2012-03-30T10:34:48Z 2012-01-23T09:24:33Z 2012-03-30T10:34:48Z 2012-03 Thesis http://hdl.handle.net/10019.1/20024 en_ZA Stellenbosch University 64 p. : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | SOD Superoxide dismutase Nitric oxide Cystatin Salt stress in plants Soybean Nodules Dissertations -- Plant biotechnology Theses -- Plant biotechnology Dissertations -- Genetics Theses -- Genetics Jacobs, Frans Alexander Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title | Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title_full | Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title_fullStr | Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title_full_unstemmed | Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title_short | Identification of regulatory elements mediating responses of SOD and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| title_sort | identification of regulatory elements mediating responses of sod and cystatin transcripts to salt stress and nitric oxide in soybean nodules |
| topic | SOD Superoxide dismutase Nitric oxide Cystatin Salt stress in plants Soybean Nodules Dissertations -- Plant biotechnology Theses -- Plant biotechnology Dissertations -- Genetics Theses -- Genetics |
| url | http://hdl.handle.net/10019.1/20024 |
| work_keys_str_mv | AT jacobsfransalexander identificationofregulatoryelementsmediatingresponsesofsodandcystatintranscriptstosaltstressandnitricoxideinsoybeannodules |