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The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes
Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2009
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| _version_ | 1867613734012190720 |
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| access_status_str | Open Access |
| author | Perold, Helene |
| author2 | Swart, Amanda C. |
| author_browse | Perold, Helene Swart, Amanda C. |
| author_facet | Swart, Amanda C. Perold, Helene |
| author_sort | Perold, Helene |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/2381 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:40:50.669Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/2381 The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes Perold, Helene Swart, Amanda C. Swart, P. University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. Aspalathus linearis Cytochrome P-450 Adrenal steroidogenesis Flavonoids Enzymes Metalloenzymes Dissertations -- Biochemistry Theses -- Biochemistry Thesis (MSc (Biochemistry))--University of Stellenbosch, 2009. This study: 1. Describes the preparation of unfermented and fermented rooibos methanol and aqueous extracts. 2. Investigates the influence of unfermented and fermented rooibos methanol and aqueous extracts on the binding of natural steroid substrates to ovine adrenal microsomal cytochrome P450 enzymes, demonstrating that the binding of natural steroids is inhibited in the presence of rooibos extracts. 3. Describes an assay demonstrating the inhibitory effect of rooibos extracts on the catalytic activity of cytochrome 17α-hydroxylase (CYP17) and cytochrome 21-hydroxylase (CYP21) in ovine adrenal microsomes. 4. Investigates the influence of unfermented and fermented rooibos methanol extracts on the catalytic activity of individual cytochrome P450 enzymes – CYP17 and baboon CYP21, that are expressed in COS1 cells. 5. Demonstrates that fractions of the unfermented rooibos methanol extract inhibits the binding of natural steroid substrate to microsomal cytochrome P450 enzymes as well as the catalytic activity of baboon CYP21 expressed in COS1 cells. 6. Investigates the inhibitory influence of individual rooibos flavonoids on the catalytic activity of baboon CYP21 expressed in COS1 cells. 2009-03-04T05:58:50Z 2010-06-01T08:47:30Z 2009-03-04T05:58:50Z 2010-06-01T08:47:30Z 2009-03 Thesis http://hdl.handle.net/10019.1/2381 en University of Stellenbosch application/pdf application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Aspalathus linearis Cytochrome P-450 Adrenal steroidogenesis Flavonoids Enzymes Metalloenzymes Dissertations -- Biochemistry Theses -- Biochemistry Perold, Helene The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title | The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title_full | The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title_fullStr | The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title_full_unstemmed | The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title_short | The influence of Rooibos (Aspalathus linearis) on adrenal steroidogenic P450 enzymes |
| title_sort | influence of rooibos aspalathus linearis on adrenal steroidogenic p450 enzymes |
| topic | Aspalathus linearis Cytochrome P-450 Adrenal steroidogenesis Flavonoids Enzymes Metalloenzymes Dissertations -- Biochemistry Theses -- Biochemistry |
| url | http://hdl.handle.net/10019.1/2381 |
| work_keys_str_mv | AT peroldhelene theinfluenceofrooibosaspalathuslinearisonadrenalsteroidogenicp450enzymes AT peroldhelene influenceofrooibosaspalathuslinearisonadrenalsteroidogenicp450enzymes |