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Analysis of dextrin dextranase from Gluconobacter oxydans
Thesis (MSc (Genetics. Institute for Plant Biotechnology (IPB)))--Stellenbosch University, 2008.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : Stellenbosch University
2008
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| _version_ | 1867613887108481024 |
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| access_status_str | Open Access |
| author | Van Wyk, Nathan |
| author2 | Lloyd, James Richard |
| author_browse | Lloyd, James Richard Van Wyk, Nathan |
| author_facet | Lloyd, James Richard Van Wyk, Nathan |
| author_sort | Van Wyk, Nathan |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc (Genetics. Institute for Plant Biotechnology (IPB)))--Stellenbosch University, 2008. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/2619 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:16.997Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/2619 Analysis of dextrin dextranase from Gluconobacter oxydans Van Wyk, Nathan Lloyd, James Richard Kossmann, J. M. Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Institute for Plant Biotechnology. Dextrin dextranase Gluconobacter oxydans Theses -- Plant biotechnology Dissertations -- Plant biotechnology Thesis (MSc (Genetics. Institute for Plant Biotechnology (IPB)))--Stellenbosch University, 2008. Dextran is a high value glucose polymer used in medicine and an array of laboratory techniques. It is synthesised by lactic-acid bacteria from sucrose but has also reportedly been produced by Gluconobacter oxydans (G. oxydans) from a range of maltooligosaccharides (MOS) via the action of dextrin dextranase (DDase). In this study the presence of DDase is investigated in two G. oxydans strains (ATCC 621H and ATCC 19357) and shown to be present in the ATCC 19357 strain, but not in the ATCC 621H strain. The enzyme was partially purified from the ATCC 19357 strain, and its kinetic properties investigated. The partially purified protein was also digested with trypsin, and de novo peptide sequences obtained from it. Several attempts were made to obtain the gene coding for the DDase. These include amplifying an open reading frame from the G. oxydans genome coding for a glycosyltransferase with the approximate molecular weight of the DDase, using the peptide sequences obtained from the partially purified protein to design degenerate PCR primers and the production of a genomic DNA library for functional screening in E. coli. None of these approaches led to the successful isolation of the extracellular DDase sequence. Masters 2008-11-21T06:50:50Z 2010-06-01T08:53:46Z 2008-11-21T06:50:50Z 2010-06-01T08:53:46Z 2008-12 Thesis http://hdl.handle.net/10019.1/2619 en Stellenbosch University application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Dextrin dextranase Gluconobacter oxydans Theses -- Plant biotechnology Dissertations -- Plant biotechnology Van Wyk, Nathan Analysis of dextrin dextranase from Gluconobacter oxydans |
| title | Analysis of dextrin dextranase from Gluconobacter oxydans |
| title_full | Analysis of dextrin dextranase from Gluconobacter oxydans |
| title_fullStr | Analysis of dextrin dextranase from Gluconobacter oxydans |
| title_full_unstemmed | Analysis of dextrin dextranase from Gluconobacter oxydans |
| title_short | Analysis of dextrin dextranase from Gluconobacter oxydans |
| title_sort | analysis of dextrin dextranase from gluconobacter oxydans |
| topic | Dextrin dextranase Gluconobacter oxydans Theses -- Plant biotechnology Dissertations -- Plant biotechnology |
| url | http://hdl.handle.net/10019.1/2619 |
| work_keys_str_mv | AT vanwyknathan analysisofdextrindextranasefromgluconobacteroxydans |