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A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line
Thesis (MSc (Physiological Sciences))--University of Stellenbosch, 2009.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2009
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| _version_ | 1867614098032689152 |
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| access_status_str | Open Access |
| author | Van den Heever, Martine |
| author2 | Engelbrecht, Anna-Mart |
| author_browse | Engelbrecht, Anna-Mart Van den Heever, Martine |
| author_facet | Engelbrecht, Anna-Mart Van den Heever, Martine |
| author_sort | Van den Heever, Martine |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Physiological Sciences))--University of Stellenbosch, 2009. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/2970 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:46:38.139Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/2970 A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line Van den Heever, Martine Engelbrecht, Anna-Mart Loos, Benjamin University of Stellenbosch. Faculty of Science. Dept. of Physiological Sciences. Dissertations -- Physiology (Human and animal) Theses -- Physiology (Human and animal) Curcuma -- Therapeutic use Apoptosis -- Treatment Necrosis -- Treatment Adenocarcinoma -- Treatment Cancer -- Treatment Thesis (MSc (Physiological Sciences))--University of Stellenbosch, 2009. Breast cancer is currently the primary cause of cancer-related death in women worldwide. Conventional treatments such as radiation and chemotherapy have many deleterious and long lasting side-effects, some of which are permanent, such as infertility. As certain tumour cells can also acquire resistance to chemotherapy, the need for the development of a less severe, yet more effective, targeted anti-cancer treatment exists. Curcumin, a plant polyphenol from Curcuma longa, has long been thought to possess antitumour, antioxidant, anti-arthritic, anti-amyloid, anti-ischemic and anti-inflammatory properties. Numerous studies conducted over the past sixty years confirm this. We aimed at examining the effect of curcumin on cell viability and the different modes of cell death, namely apoptosis, necrosis and autophagy, in the MCF-12A (non-tumorigenic mammary epithelial) and MCF-7 (mammary adenocarcinoma) cell lines. Cells were incubated with different doses of curcumin to evaluate the dose response through a MTT assay. Thereafter, cells were incubated with 200 μM curcumin for 48 hrs and stained with markers and DNA stains for apoptosis (Hoechst, Caspase-3, PARP), necrosis (Propidium Iodide) and autophagy (LC3B and Beclin-1). Cells were examined via fluorescence microscopy, Western Blot- and FACS analyses. MTT results showed no significant decrease in viability in the MCF-12A cell line after curcumin treatment. However, a significant decrease in viability was observed in MCF-7 cells after treatment with 200 μM curcumin (p < 0.05). Treated MCF-7 cells also show clear LC3B expression. FACS results show a significant difference in Hoechst mean fluorescence intensity in MCF-7 cells after curcumin treatment (p < 0.05). This study provides evidence that MCF-7 cells respond to a 200 μM dose of curcumin treatment through metabolic change and induction of the autophagic pathway. The model system used in this study provides groundwork for further cell culture based studies regarding breast cancer and curcumin. 2009-02-23T13:09:28Z 2010-06-01T09:02:49Z 2009-02-23T13:09:28Z 2010-06-01T09:02:49Z 2009-03 Thesis http://hdl.handle.net/10019.1/2970 en University of Stellenbosch application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Dissertations -- Physiology (Human and animal) Theses -- Physiology (Human and animal) Curcuma -- Therapeutic use Apoptosis -- Treatment Necrosis -- Treatment Adenocarcinoma -- Treatment Cancer -- Treatment Van den Heever, Martine A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title | A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title_full | A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title_fullStr | A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title_full_unstemmed | A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title_short | A comparison of the effect of curcumin treatment on apoptosis, necrosis and autophagy in a MCF-7 mammary adenocarcinoma and a MCF-12A healthy mammary epithelial cell line |
| title_sort | comparison of the effect of curcumin treatment on apoptosis necrosis and autophagy in a mcf 7 mammary adenocarcinoma and a mcf 12a healthy mammary epithelial cell line |
| topic | Dissertations -- Physiology (Human and animal) Theses -- Physiology (Human and animal) Curcuma -- Therapeutic use Apoptosis -- Treatment Necrosis -- Treatment Adenocarcinoma -- Treatment Cancer -- Treatment |
| url | http://hdl.handle.net/10019.1/2970 |
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