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A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1)
Thesis (MSc (Biochemistry))--University of Stellenbosch, 2005.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2006
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| _version_ | 1867613907507478528 |
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| access_status_str | Open Access |
| author | Storbeck, Karl-Heinz |
| author2 | Swart, Amanda C. |
| author_browse | Storbeck, Karl-Heinz Swart, Amanda C. |
| author_facet | Swart, Amanda C. Storbeck, Karl-Heinz |
| author_sort | Storbeck, Karl-Heinz |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Biochemistry))--University of Stellenbosch, 2005. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/3113 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:36.390Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/3113 A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) Storbeck, Karl-Heinz Swart, Amanda C. Swart, P. University of Stellenbosch. Faculty of Science. Dept. of Biochemistry. Dissertations -- Biochemistry Theses -- Biochemistry Cytochrome P-450 Mutagenesis Adrenocortical hormone Homology (Biology) Amino acids Baboons -- Cytogenetics Molecular cloning Thesis (MSc (Biochemistry))--University of Stellenbosch, 2005. This study describes: 1. The cloning of baboon cytochrome P450 side-chain cleavage (CYP11A1) cDNA by in vitro site-directed mutagenesis. 2. The identification and sequencing of three baboon CYP11A1 mutants: CYP11A1a, CYP11A1b and CYP11A1c. 3. The expression and characterisation of baboon and human CYP11A1 cDNA, CYP11A1a, CYP11A1b and CYP11A1c in nonsteroidogenic COS-1 cells. The Km and V-values for the metabolism of 25-hydroxycholesterol were determined. 4. The construction of the first homology model of CYP11A1, using both mammalian (CYP2C5) and bacterial (CYP102) cytochromes P450 crystal structures as templates. 5. A structure/function study into the role of the amino acid residues Ile98, Lys103 and Thr291 in substrate binding and enzymatic activity. 6. The proposal of a topological model of the CYP11A1 active pocket as determined by substrate docking studies. 2006-11-15T09:02:30Z 2010-06-01T09:06:37Z 2006-11-15T09:02:30Z 2010-06-01T09:06:37Z 2005-12 Thesis http://hdl.handle.net/10019.1/3113 en University of Stellenbosch 7447068 bytes application/pdf application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Dissertations -- Biochemistry Theses -- Biochemistry Cytochrome P-450 Mutagenesis Adrenocortical hormone Homology (Biology) Amino acids Baboons -- Cytogenetics Molecular cloning Storbeck, Karl-Heinz A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title | A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title_full | A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title_fullStr | A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title_full_unstemmed | A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title_short | A structure/function investigation into baboon cytochrome P450 side-chain cleavage (CYP11A1) |
| title_sort | structure function investigation into baboon cytochrome p450 side chain cleavage cyp11a1 |
| topic | Dissertations -- Biochemistry Theses -- Biochemistry Cytochrome P-450 Mutagenesis Adrenocortical hormone Homology (Biology) Amino acids Baboons -- Cytogenetics Molecular cloning |
| url | http://hdl.handle.net/10019.1/3113 |
| work_keys_str_mv | AT storbeckkarlheinz astructurefunctioninvestigationintobabooncytochromep450sidechaincleavagecyp11a1 AT storbeckkarlheinz structurefunctioninvestigationintobabooncytochromep450sidechaincleavagecyp11a1 |