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Virus induced gene silencing for the study of starch metabolism
Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2010
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| _version_ | 1867613777963253760 |
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| access_status_str | Open Access |
| author | George, Gavin M. (Gavin Mager) |
| author2 | Lloyd, James Richard |
| author_browse | George, Gavin M. (Gavin Mager) Lloyd, James Richard |
| author_facet | Lloyd, James Richard George, Gavin M. (Gavin Mager) |
| author_sort | George, Gavin M. (Gavin Mager) |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/4024 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:41:32.562Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/4024 Virus induced gene silencing for the study of starch metabolism George, Gavin M. (Gavin Mager) Lloyd, James Richard Kossmann, J. M. University of Stellenbosch. Faculty of Agrisciences. Dept. of Genetics. Institute for Plant Biotechnology (IPB) Starch metabolism Plastidial pyrophosphate VIGS Dissertations -- Plant biotechnology Theses -- Plant biotechnology Plant biotechnology Gene silencing Thesis (PhD (Plant Biotechnology))--University of Stellenbosch, 2010. ENGLISH ABSTRACT: Virus Induced Gene Silencing (VIGS) was optimized to allow for the study of starch metabolism. The plastidial inorganic pyrophosphatase gene, for which a mutant has never been identified, was studied using VIGS and it was found to have a broad role in this subcellular compartment. The accumulation of inorganic pyrophosphate limited the production of starch, carotenoids, chlorophyll, and increased the plants susceptibility to drought stress. These effects highlight the importance of this enzyme in maintaining a low intraplastidial concentration of PPi providing an environment which facilitates these anabolic processes. Several genes involved in starch synthesis and degradation were also targeted with the aim of establishing a system of multiple gene silencing for the study of metabolic pathways. One, two and three genes were successfully silenced using this system which was validated based on previously published data. Interestingly, simultaneous silencing of the two isoforms of disproportionating enzyme led to a novel phenotype as a large reduction in starch instead of the expected increase was observed. No Afrikaans abstract available Doctoral 2010-02-03T10:08:22Z 2010-08-13T13:11:26Z 2010-02-03T10:08:22Z 2010-08-13T13:11:26Z 2010-03 Thesis http://hdl.handle.net/10019.1/4024 en University of Stellenbosch 124 p. : ill. application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Starch metabolism Plastidial pyrophosphate VIGS Dissertations -- Plant biotechnology Theses -- Plant biotechnology Plant biotechnology Gene silencing George, Gavin M. (Gavin Mager) Virus induced gene silencing for the study of starch metabolism |
| title | Virus induced gene silencing for the study of starch metabolism |
| title_full | Virus induced gene silencing for the study of starch metabolism |
| title_fullStr | Virus induced gene silencing for the study of starch metabolism |
| title_full_unstemmed | Virus induced gene silencing for the study of starch metabolism |
| title_short | Virus induced gene silencing for the study of starch metabolism |
| title_sort | virus induced gene silencing for the study of starch metabolism |
| topic | Starch metabolism Plastidial pyrophosphate VIGS Dissertations -- Plant biotechnology Theses -- Plant biotechnology Plant biotechnology Gene silencing |
| url | http://hdl.handle.net/10019.1/4024 |
| work_keys_str_mv | AT georgegavinmgavinmager virusinducedgenesilencingforthestudyofstarchmetabolism |