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Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives
Thesis (MSc)--Stellenbosch University, 2009.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613979029798913 |
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| access_status_str | Open Access |
| author | Ramogola, Watsie Princess Neo |
| author2 | Makunga, Nokwanda |
| author_browse | Makunga, Nokwanda Ramogola, Watsie Princess Neo |
| author_facet | Makunga, Nokwanda Ramogola, Watsie Princess Neo |
| author_sort | Ramogola, Watsie Princess Neo |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2009. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/40563 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:44:44.746Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/40563 Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives Ramogola, Watsie Princess Neo Makunga, Nokwanda Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics. Salvia africana-lutea aromatic herb -- Antimicrobial bioactivity Salvia africana-lutea -- Molecular analysis Salvia africana-lutea -- Medicinal use Theses -- Genetics Dissertations -- Genetics Thesis (MSc)--Stellenbosch University, 2009. ENGLISH ABSTRACT: Biotechnological applications are useful for adding value to the local medicinal plants and may provide an alternative source of pharmacologically-active compounds thus assisting with the conservation of wild populations. Salvia africana-lutea aromatic herb has long been used in folk medicine by traditional healers in the Western Cape Province (South Africa) for various ailments. As an in vitro conservation strategy, a continuous S. africana-lutea shoot culture was successfully established in solid MS medium containing BA (0.5 mg L-1) and NAA (0.2 mg L-1). The regeneration rate of the S. africana-lutea plants was high which produced approximately 720 plantlets in 20 culture bottles over a four week cycle. The microshoots were rooted in the MS medium without PGRs prior to acclimatisation. A survival rate of 92% was recorded for the greenhouse-acclimatised shoots. AFRIKAANS OPSOMMING: Biotegnologiese toepassings is nuttig vir waarde toevoeging tot inheemse medisinale plante en kan ‘n alternatiewe bron van farmakologies aktiewe verbindings verskaf wat bydrae tot die bewaring van populasies in die natuur. Die aromatiese krui Salvia. Africana-lutea is reeds vir ‘n lang tydperk in volks medisyne deur tradisionele geneesheers in die Wes Kaap provinsie (Suid Afrika) vir ‘n verskeidenheid kwale gebruik. ‘n Kontinu S. africana-lutea lootkultuur in soliede Murashige en Skoog (1962) (MS) media wat BA (0.5 mg L-1) en NAA (0.2 mg L-1) bevat, is suksesvol as ‘n in vitro konservasie strategie ontwikkel. Die regenerasie tempo van die S. africana-lutea plante was hoog en het ongeveer 720 plante in 20 kultuur bottels tydens ‘n vier week siklus gelewer. Die mikrolote is op plant groei reguleerder vrye MS media gewortel voordat plante geaklimatiseer is. ’n Oorlewingstempo van 92% is vir die glashuis geaklimatiseerde lote waargeneem. Masters 2012-08-10T17:08:13Z 2012-08-10T17:08:13Z 2009-12 Thesis http://hdl.handle.net/10019.1/40563 en_ZA Stellenbosch University xxvi, 160 p. : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Salvia africana-lutea aromatic herb -- Antimicrobial bioactivity Salvia africana-lutea -- Molecular analysis Salvia africana-lutea -- Medicinal use Theses -- Genetics Dissertations -- Genetics Ramogola, Watsie Princess Neo Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title | Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title_full | Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title_fullStr | Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title_full_unstemmed | Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title_short | Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives |
| title_sort | molecular analyses of salvia africana lutea l transgenic hairy root clones for secondary bioactives |
| topic | Salvia africana-lutea aromatic herb -- Antimicrobial bioactivity Salvia africana-lutea -- Molecular analysis Salvia africana-lutea -- Medicinal use Theses -- Genetics Dissertations -- Genetics |
| url | http://hdl.handle.net/10019.1/40563 |
| work_keys_str_mv | AT ramogolawatsieprincessneo molecularanalysesofsalviaafricanalutealtransgenichairyrootclonesforsecondarybioactives |