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Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes
Thesis (MSc)--Stellenbosch University, 2005.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613734843711488 |
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| access_status_str | Open Access |
| author | Wepener, Ilse |
| author2 | Swart, P. |
| author_browse | Swart, P. Wepener, Ilse |
| author_facet | Swart, P. Wepener, Ilse |
| author_sort | Wepener, Ilse |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2005. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/50418 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:40:51.455Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/50418 Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes Wepener, Ilse Swart, P. Swart, Amanda C. Stellenbosch University. Faculty of Science. Dept. of Biochemistry. Pichia pastoris Genetic vectors Yeast fungi Cytochrome P-450 Dissertations -- Biochemistry Theses -- Biochemistry Thesis (MSc)--Stellenbosch University, 2005. ENGLISH ABSTRACT: This study describes: I. The cloning of the CVP 19 gene and construction of the intracellular expression vector pPIC3.5K-CYP19. II. The transformation of the yeast, Pichia pastoris with the constructed vector. III. The expression ofP450arom in Pichia pastoris. IV. The determination of enzyme activity and isolation of the protein from the Pichia pastoris cells. V. The expression of P450c 17 in Pichia pastoris. VI. The determination of kinetic constants for the conversion of progesterone to 170H-progesterone and 160H-progesterone by P450c17. AFRIKAANSE OPSOMMING: Hierdie studie beskryf: I. Die klonering van die CVP 19 geen en die konstruksie van die intrasellulêre uitdrukkingsplasmied, pPIC3.5K-CYPI9. II. Die transformasie van die gis, Pichia pastoris, met die gekonstrueerde plasmied. III. Die uitdrukking van aromatase in Pichia pastoris. IV. Die bepaling van ensiemaktiwiteit en die isolering van die proteïen vanuit Pichia pastoris. V. Die uitdrukking van P450c17 in Pichia pastoris. VI. Die bepaling van kinetiese konstantes vir die omsetting van progesteroon na 170H-progesteroon en 160H-progesteroon deur P450c17. 2012-08-27T11:33:24Z 2012-08-27T11:33:24Z 2005-12 Thesis http://hdl.handle.net/10019.1/50418 en_ZA Stellenbosch University 93 p. : ill. (some col.) application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Pichia pastoris Genetic vectors Yeast fungi Cytochrome P-450 Dissertations -- Biochemistry Theses -- Biochemistry Wepener, Ilse Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title | Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title_full | Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title_fullStr | Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title_full_unstemmed | Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title_short | Pichia pastoris : a viable expression system for steroidogenic cytochrome P450 enzymes |
| title_sort | pichia pastoris a viable expression system for steroidogenic cytochrome p450 enzymes |
| topic | Pichia pastoris Genetic vectors Yeast fungi Cytochrome P-450 Dissertations -- Biochemistry Theses -- Biochemistry |
| url | http://hdl.handle.net/10019.1/50418 |
| work_keys_str_mv | AT wepenerilse pichiapastorisaviableexpressionsystemforsteroidogeniccytochromep450enzymes |