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Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize
Thesis (MSc (Plant Biotechnology))--University of Stellenbosch, 2010.
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| Format: | Thesis |
| Language: | English |
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Stellenbosch : University of Stellenbosch
2010
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| _version_ | 1867613961816375296 |
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| access_status_str | Open Access |
| author | Bilibana, Mawethu Pascoe |
| author2 | Ludidi, N. N. |
| author_browse | Bilibana, Mawethu Pascoe Ludidi, N. N. |
| author_facet | Ludidi, N. N. Bilibana, Mawethu Pascoe |
| author_sort | Bilibana, Mawethu Pascoe |
| collection | Thesis |
| dc_rights_str_mv | University of Stellenbosch |
| description | Thesis (MSc (Plant Biotechnology))--University of Stellenbosch, 2010. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/5221 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:44:27.789Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| publisher | Stellenbosch : University of Stellenbosch |
| publisherStr | Stellenbosch : University of Stellenbosch |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/5221 Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize Bilibana, Mawethu Pascoe Ludidi, N. N. University of Stellenbosch. Faculty of Agrisciences. Dept. of Genetics. Institute for Plant Biotechnology. Soybean Maize Cysteine protease Nitric oxide signaling Dissertations -- Plant biotechnology Theses -- Plant biotechnology Corn Abiotic stress Thesis (MSc (Plant Biotechnology))--University of Stellenbosch, 2010. ABSTRACT: Nitric Oxide (NO) is an essential bioregulatory molecule in plant growth, development, and tolerance against biotic and abiotic stresses. In legume root nodules, abiotic stresses impose restraint on metabolic capacity of bacteria and cause oxidative damage to cellular macromolecules, leading to inhibition of nitrogenase activity. In this study, the primary aim was to determine the influence of NO signaling on cysteine protease activity in soybean (Glycine. max [L] Merr) root nodules. Intact plants were treated with a NO donor, diethylenetriamine/nitric oxide adjunct (DETA/NO), 8-(4-chlorophenylthio)-[CPT]- cGMP, sorbitol and sodium chloride (NaCl). The root nodule cysteine protease activity was measured using the chromogenic substrate N-benzoil-L-p-nitroanilide (L-BAPNA). The results demonstrated NO as acting both as a protection against programmed cell death (PCD) at low exogenously applied NO concentrations, or as inducing PCD through regulating the cysteine proteases activity in root nodules when NO is applied at elevated concentrations. In the root nodules, the activity of cysteine protease is regulated either through cyclic guanosine monophosphate (cGMP)-dependent during abiotic stress or cGMP-independent pathways during normal root nodule development. The purpose of this research was to highlight the importance of NO in cell signaling and cysteine protease activity in legume root nodules. We also focused on the effect of abiotic stress on two maize genotypes as well as the influence of abiotic stress on cysteine protease activity in the abiotic stress-sensitive maize genotype than the tolerant genotype. The study suggests that cysteine protease activity can be used as early screen to identify abiotic stress-sensitive/tolerant maize genotype upon exposure to abiotic stress. AFRIKAANSE OPSOMMING: Geen Afrikaanse opsomming beskikbaar. National Research Foundation Masters 2010-11-24T06:56:23Z 2010-11-24T06:56:23Z 2010-11-24 Thesis http://hdl.handle.net/10019.1/5221 en University of Stellenbosch 82 p. : ill. application/pdf Stellenbosch : University of Stellenbosch |
| spellingShingle | Soybean Maize Cysteine protease Nitric oxide signaling Dissertations -- Plant biotechnology Theses -- Plant biotechnology Corn Abiotic stress Bilibana, Mawethu Pascoe Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title | Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title_full | Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title_fullStr | Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title_full_unstemmed | Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title_short | Nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| title_sort | nitric oxide signaling and cysteine protease activity in the modulation of abiotic stress responses in soybean and maize |
| topic | Soybean Maize Cysteine protease Nitric oxide signaling Dissertations -- Plant biotechnology Theses -- Plant biotechnology Corn Abiotic stress |
| url | http://hdl.handle.net/10019.1/5221 |
| work_keys_str_mv | AT bilibanamawethupascoe nitricoxidesignalingandcysteineproteaseactivityinthemodulationofabioticstressresponsesinsoybeanandmaize |