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Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment
Thesis (MSc)--Stellenbosch University, 2003.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867613957727977472 |
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| access_status_str | Open Access |
| author | Douglas, C. A. (Claire Anne) |
| author2 | Sandra, P. J. F. |
| author_browse | Douglas, C. A. (Claire Anne) Sandra, P. J. F. |
| author_facet | Sandra, P. J. F. Douglas, C. A. (Claire Anne) |
| author_sort | Douglas, C. A. (Claire Anne) |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (MSc)--Stellenbosch University, 2003. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/53249 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:44:24.378Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/53249 Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment Douglas, C. A. (Claire Anne) Sandra, P. J. F. Lynen, F. Stellenbosch University. Faculty of Science. Dept. of Chemistry & Polymer Science . Liquid chromatography High performance liquid chromatography Amino acids -- Analysis Dissertations -- Chemistry Theses -- Chemistry Thesis (MSc)--Stellenbosch University, 2003. ENGLISH ABSTRACT: In this study, the analysis of ammo acids usmg High Performance Liquid Chromatography (HPLC) with pre-column derivatisation was optimised. The derivatisation reagents include o-phthaldialdehyde (OPA), 9- fluorenylmethylchloroformate (FMOC) and iodoacetic acid (IDA). Detection was performed using UV and fluorescence in series. The developed method was utilised for the analysis and quantitation of amino acids in eighteen wines. The application of chemometric data evaluation was initiated. AFRIKAANSE OPSOMMING: Hierdie ondersoek behels die optimisering van die aminosuuranalise deur gebruik te maak van Hoë Druk Vloeistof Chromatografie (HDVC) in kombinasie met pre-kolom derivatisering. Die derivativatiserings reagense sluit in o-phthaldialdehied (OPA), 9- fluorenielmetielchloroformaat (FMOC) en jodoasynsuur (IDA). Deteksie is gedoen deur gebruik gemaak van 'n ultraviolet (UV) en 'n fluorosensie detektor in serie. Die metode sodoende ontwikkel is gebruik vir die analise en kwantifisering van aminosure in agtien wyne. Die toepassing van chemometriese data evaluasie is ook geïnisieer. 2012-08-27T11:35:22Z 2012-08-27T11:35:22Z 2003-12 Thesis http://hdl.handle.net/10019.1/53249 en_ZA Stellenbosch University 95 p. : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Liquid chromatography High performance liquid chromatography Amino acids -- Analysis Dissertations -- Chemistry Theses -- Chemistry Douglas, C. A. (Claire Anne) Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title | Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title_full | Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title_fullStr | Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title_full_unstemmed | Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title_short | Amino acid analysis in wines by liquid chromatography : UV and fluorescence detection without sample enrichment |
| title_sort | amino acid analysis in wines by liquid chromatography uv and fluorescence detection without sample enrichment |
| topic | Liquid chromatography High performance liquid chromatography Amino acids -- Analysis Dissertations -- Chemistry Theses -- Chemistry |
| url | http://hdl.handle.net/10019.1/53249 |
| work_keys_str_mv | AT douglascaclaireanne aminoacidanalysisinwinesbyliquidchromatographyuvandfluorescencedetectionwithoutsampleenrichment |