Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393)
Thesis (M. Sc.) -- University of Stellenbosch, 1995.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Stellenbosch : Stellenbosch University
2012
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613904878698496 |
|---|---|
| access_status_str | Open Access |
| author | Du Plessis, Erika Magarete |
| author2 | Dicks, L. M. T. |
| author_browse | Dicks, L. M. T. Du Plessis, Erika Magarete |
| author_facet | Dicks, L. M. T. Du Plessis, Erika Magarete |
| author_sort | Du Plessis, Erika Magarete |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc.) -- University of Stellenbosch, 1995. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/54742 |
| institution | Stellenbosch University (South Africa) |
| language | English |
| last_indexed | 2026-06-10T12:43:33.723Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/54742 Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) Du Plessis, Erika Magarete Dicks, L. M. T. Stellenbosch University. Faculty of Science. Dept. of Microbiology. Lactobacillus -- Genetics Lactobacillus -- Classification Dissertations -- Microbiology Thesis (M. Sc.) -- University of Stellenbosch, 1995. The genotypic relatedness among strains of Lactobacillus acidophilus and phenotypically closely related species (often referred to as members of the “L. acidophilus-group”), and strains of the “Lactobacillus casei-group” (including strains from phenotypically closely related Lactobacillus rhamnosus and Lactobacillus paracasei) were studied using numerical analysis of total soluble cell protein patterns, NAD-dependent L(+)-lactic dehydrogenase (nLDH) electrophoresis, and random amplified polymorphic DNA (RAPD)-PCR analysis. Two independent studies were undertaken on the “L. acidophilus”- and “L. casei”- groups. Eighteen strains of L. acidophilus grouped into three protein profile clusters at r ≥ 0.50, separate from the type strain of L. acidophilus (NCFB 1748ᵀ, ATCC 4356ᵀ), suggesting that the species consists of a genetically heterogeneous collection of strains. Three strains of L. acidophilus (J-2012C, A 21 and ATCC 11506) clustered with the type strain of Lactobacillus crispatus (NCFB 2752ᵀ) in the same protein profile cluster, whereas the majority of strains of L. acidophilus formed a tight cluster with the type strains of Lactobacillus amylovorus (NRRL B4540ᵀ) and Lactobacillus gallinarum (NCFB 2235ᵀ). However, the protein- and L(+)-nLDH profiles of L. gallinarum NCFB 2235ᵀ were different from that recorded for the other strains in the same protein profile cluster. Strain NCFB 2235ᵀ is therefore regarded as an intruder in this cluster. Four strains of L. acidophilus (VPI 0824, Mav1 I 2311C, 104 and VPI 9A270C) grouped in a third protein profile cluster. The type strains of Lactobacillus gasseri (NCFB 2233ᵀ) and Lactobacillus johnsonii (NCFB 2242ᵀ) did not group into any cluster, confirming their status as separate and well defined species. Twelve strains, resembling L. casei and subspecies of L. paracasei, grouped into one protein profile cluster. The overall protein profile of the type strain of L. rhamnosus (NCFB 2676ᵀ) was different from all other strains included in this study, confirming its status as a separate and well defined species. The type strain of L. casei subsp. casei (ATCC 393ᵀ) shares a low DNA homology with other strains of L. casei subsp. casei (18–46%) and L. paracasei (30–50%), but has a high DNA similarity (80%) with L. zeae ATCC 15820, the original type strain of “Lactobacterium zeae” Kuznetsov 1959. The RAPD-PCR profile of strain ATCC 393ᵀ is also different from the profiles recorded for the other species. On the other hand, L. casei ATCC 334 is genetically closely related to strains of L. casei subsp. casei (97%) and L. paracasei (71–91%), group in the same protein profile cluster, and share several DNA amplicons with strains of L. paracasei. Based on these results, we propose reclassification of L. casei subsp. casei ATCC 393ᵀ and L. rhamnosus ATCC 15820 as Lactobacillus zeae nom. rev. (type = ATCC 15820), the designation of ATCC 334 as neotype of L. casei subsp. casei, and rejection of the name L. paracasei. Results obtained by RAPD-PCR analyses corresponded well with results obtained by numerical analyses of total soluble cell protein patterns. RAPD-PCR analysis proved valuable in differentiating strains of L. casei and L. acidophilus from phenotypically closely related species. RAPD-PCR analyses will have to be done on other species and genera of lactic acid bacteria to confirm this statement. Masters 2012-08-27T11:36:42Z 2012-08-27T11:36:42Z 1995 Thesis http://hdl.handle.net/10019.1/54742 en Stellenbosch University 64 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Lactobacillus -- Genetics Lactobacillus -- Classification Dissertations -- Microbiology Du Plessis, Erika Magarete Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title | Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title_full | Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title_fullStr | Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title_full_unstemmed | Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title_short | Evaluation of random amplified polymorphic DNA (RAPD)-PCR as a method to differentiate species of the Lactobacillus acidophilus-group and to determine the taxonomic status of the type strain of Lactobacillus casei subsp. casei (ATCC 393) |
| title_sort | evaluation of random amplified polymorphic dna rapd pcr as a method to differentiate species of the lactobacillus acidophilus group and to determine the taxonomic status of the type strain of lactobacillus casei subsp casei atcc 393 |
| topic | Lactobacillus -- Genetics Lactobacillus -- Classification Dissertations -- Microbiology |
| url | http://hdl.handle.net/10019.1/54742 |
| work_keys_str_mv | AT duplessiserikamagarete evaluationofrandomamplifiedpolymorphicdnarapdpcrasamethodtodifferentiatespeciesofthelactobacillusacidophilusgroupandtodeterminethetaxonomicstatusofthetypestrainoflactobacilluscaseisubspcaseiatcc393 |