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The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs).
Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1996.
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| Format: | Thesis |
| Language: | en_ZA |
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Stellenbosch : Stellenbosch University
2012
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| _version_ | 1867614018484568064 |
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| access_status_str | Open Access |
| author | Bester, Aletta Elizabeth |
| author2 | Retief, A. E. |
| author_browse | Bester, Aletta Elizabeth Retief, A. E. |
| author_facet | Retief, A. E. Bester, Aletta Elizabeth |
| author_sort | Bester, Aletta Elizabeth |
| collection | Thesis |
| dc_rights_str_mv | Stellenbosch University |
| description | Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1996. |
| format | Thesis |
| id | oai:scholar.sun.ac.za:10019.1/55290 |
| institution | Stellenbosch University (South Africa) |
| language | en_ZA |
| last_indexed | 2026-06-10T12:45:21.489Z |
| license_str | Other — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from SUNScholar — Stellenbosch University Repository |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Stellenbosch : Stellenbosch University |
| publisherStr | Stellenbosch : Stellenbosch University |
| record_format | dspace |
| source_str | SUNScholar — Stellenbosch University Repository |
| spelling | oai:scholar.sun.ac.za:10019.1/55290 The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). Bester, Aletta Elizabeth Retief, A. E. Warnich, L. Stellenbosch University. Faculty of AgriSciences. Dept. of Genetics & Institute of Plant Biotechnology. Human Genome Project Human gene mapping Molecular cloning Medical genetics Human chromosomes Dissertations -- Genetics Thesis (M. Sc. Agric.) -- University of Stellenbosch, 1996. One of the major objectives of the Human Genome Project is the cloning and mapping of all disease-related genes in the human genome. In order to cloneany gene, a high density of genetic markers is necessary in the region of interest. The markers are used in linkage studies to define the precise location of the minimal interval that contains the targeted gene. In this study, 3 YAC clones were used as a starting point for cloning microsatellite markers from the chromosome area 14q32. The YACs were obtained from Genome Systems library screening service. They used the primer sets of two Genethon microsatellite (AFM) markers to screen the CEPH mega-Y AC library. After screening, PCR analysis showed that the YACs correlated with the AFM primers. The YAC clones were initially characterized through pulsed-field gel electrophoresis (PFGE), Southern analysis and fluorescent in situ hybridization (FISH). The YAC insert sizes were estimatetl on a PFGE gel and Southern analysis showed that the inserts are of human origin. FISH allowed the detection of chimerism, but only up to the resolution of the fluorescence microscope, since very small chimeric fragments can not be detected by FISH. FISH also indicated that the YACs are positive for 14q32. Masters 2012-08-27T11:36:59Z 2012-08-27T11:36:59Z 1996 Thesis http://hdl.handle.net/10019.1/55290 en_ZA Stellenbosch University 91 pages : ill. application/pdf Stellenbosch : Stellenbosch University |
| spellingShingle | Human Genome Project Human gene mapping Molecular cloning Medical genetics Human chromosomes Dissertations -- Genetics Bester, Aletta Elizabeth The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title | The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title_full | The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title_fullStr | The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title_full_unstemmed | The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title_short | The isolation of microsatellite repeats from the human chromosome band 14q32 through inter-Alu amplification of yeast artificial chromosomes (YACs). |
| title_sort | isolation of microsatellite repeats from the human chromosome band 14q32 through inter alu amplification of yeast artificial chromosomes yacs |
| topic | Human Genome Project Human gene mapping Molecular cloning Medical genetics Human chromosomes Dissertations -- Genetics |
| url | http://hdl.handle.net/10019.1/55290 |
| work_keys_str_mv | AT besteralettaelizabeth theisolationofmicrosatelliterepeatsfromthehumanchromosomeband14q32throughinteraluamplificationofyeastartificialchromosomesyacs AT besteralettaelizabeth isolationofmicrosatelliterepeatsfromthehumanchromosomeband14q32throughinteraluamplificationofyeastartificialchromosomesyacs |